Plant-derived EpCAM antigen induces protective anti-cancer response

Immunotherapy holds great promise for treatment of infectious and malignant diseases and might help to prevent the occurrence and recurrence of cancer. We produced a plant-derived tumor-associated colorectal cancer antigen EpCAM (pGA733) at high yields using two modern plant expression systems. The...

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Bibliographic Details
Published in:	Cancer Immunology, Immunotherapy Vol. 57; no. 3; pp. 317 - 323
Main Authors:	Brodzik, Robert, Spitsin, Sergei, Golovkin, Max, Bandurska, Katarzyna, Portocarrero, Carla, Okulicz, Monika, Steplewski, Zenon, Koprowski, Hilary
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer-Verlag 01-03-2008 Springer Springer Nature B.V
Subjects:	Animals Antibodies - blood Antibodies, Monoclonal - pharmacology Antibody Affinity - immunology Antibody Specificity - immunology Antigens Antigens, Neoplasm - genetics Antigens, Neoplasm - immunology Antigens, Neoplasm - isolation & purification Antineoplastic agents Baculovirus Beta vulgaris - genetics Beta vulgaris - immunology Biological and medical sciences Biotechnology Cancer Research Cancer Vaccines - administration & dosage Cancer Vaccines - immunology Cell Adhesion Molecules - genetics Cell Adhesion Molecules - immunology Cell Adhesion Molecules - isolation & purification Cell Line, Tumor Cell Proliferation - drug effects Colorectal cancer Colorectal Neoplasms - immunology Colorectal Neoplasms - therapy Epithelial Cell Adhesion Molecule Gastroenterology. Liver. Pancreas. Abdomen Gene Expression Regulation, Plant - genetics Humans Immune Sera - pharmacology Immunology Immunotherapy Immunotherapy - methods Medical sciences Medicine Medicine & Public Health Mice Mice, Inbred BALB C Mice, Nude Nicotiana - genetics Nicotiana - immunology Oncology Original Original Article Pharmacology. Drug treatments Plants, Genetically Modified - chemistry Plants, Genetically Modified - genetics Plasmids Proteins Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - isolation & purification Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tobacco Treatment Outcome Tumors Vaccines Xenograft Model Antitumor Assays United States > US Recombinant subunit vaccine Colorectal cancer Plant biotechnology Antineoplastic agent Biotechnology Rectal disease Malignant tumor Subunit Colonic disease Antigen Prevention Plant Treatment Plant biotechnology, Colorectal cancer Immunotherapy Digestive diseases Intestinal disease Recombinant vaccine Cancer
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Summary:	Immunotherapy holds great promise for treatment of infectious and malignant diseases and might help to prevent the occurrence and recurrence of cancer. We produced a plant-derived tumor-associated colorectal cancer antigen EpCAM (pGA733) at high yields using two modern plant expression systems. The full antigenic domain of EpCAM was efficiently purified to confirm its antigenic and immunogenic properties as compared to those of the antigen expressed in the baculovirus system (bGA733). Recombinant plant-derived antigen induced a humoral immune response in BALB/c mice. Sera from those mice efficiently inhibited the growth of SW948 colorectal carcinoma cells xenografted in nude mice, as compared to the EpCAM-specific mAb CO17-1A. Our results support the feasibility of producing anti-cancer recombinant vaccines using plant expression systems.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0340-7004 1432-0851
DOI:	10.1007/s00262-007-0366-4