Control of relative timing and stoichiometry by a master regulator

Developmental processes in cells require a series of complex steps. Often only a single master regulator activates genes in these different steps. This poses several challenges: some targets need to be ordered temporally, while co-functional targets may need to be synchronized in both time and expre...

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Bibliographic Details
Published in:	PloS one Vol. 10; no. 5; p. e0127339
Main Authors:	Goldschmidt, Yifat, Yurkovsky, Evgeny, Reif, Amit, Rosner, Roni, Akiva, Amit, Nachman, Iftach
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 22-05-2015 Public Library of Science (PLoS)
Subjects:	Activation Baking yeast Binding sites Biochemistry Cell cycle Cell differentiation Chromosomes Dynamic control Gene expression Gene Expression Regulation, Fungal Genes Genes, Fungal Genetic aspects Localization Meiosis Molecular biology Nuclear Proteins - genetics Nuclear Proteins - metabolism Physiological aspects Promoter Regions, Genetic Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Stoichiometry Time synchronization Transcription factors Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Yeast Israel
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Summary:	Developmental processes in cells require a series of complex steps. Often only a single master regulator activates genes in these different steps. This poses several challenges: some targets need to be ordered temporally, while co-functional targets may need to be synchronized in both time and expression level. Here we study in single cells the dynamic activation patterns of early meiosis genes in budding yeast, targets of the meiosis master regulator Ime1. We quantify the individual roles of the promoter and protein levels in expression pattern control, as well as the roles of individual promoter elements. We find a consistent expression pattern difference between a non-cofunctional pair of genes, and a highly synchronized activation of a co-functional pair. We show that dynamic control leading to these patterns is distributed between promoter, gene and external regions. Through specific reciprocal changes to the promoters of pairs of genes, we show that different genes can use different promoter elements to reach near identical activation patterns.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conceived and designed the experiments: YG EY AR IN. Performed the experiments: YG AR AA. Analyzed the data: YG EY AA RR IN. Wrote the paper: YG EY AR IN. Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0127339