Search Results - "Goldman, Yale E"

Local Cytoskeletal and Organelle Interactions Impact Molecular-Motor-Driven Early Endosomal Trafficking by Zajac, Allison L., Goldman, Yale E., Holzbaur, Erika L.F., Ostap, E. Michael

“…In the intracellular environment, motor-driven cargo must navigate a dense cytoskeletal network among abundant organelles. We investigated the effects of the…”
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Positive cardiac inotrope omecamtiv mecarbil activates muscle despite suppressing the myosin working stroke by Woody, Michael S., Greenberg, Michael J., Barua, Bipasha, Winkelmann, Donald A., Goldman, Yale E., Ostap, E. Michael

“…Omecamtiv mecarbil (OM) is a positive cardiac inotrope in phase-3 clinical trials for treatment of heart failure. Although initially described as a direct…”
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Force measurements on cargoes in living cells reveal collective dynamics of microtubule motors by Hendricks, Adam G., Holzbaur, Erika L. F., Goldman, Yale E.

“…Many cellular cargoes move bidirectionally along microtubules, driven by teams of plus- and minus-end–directed motor proteins. To probe the forces exerted on…”
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Differential Regulation of Dynein and Kinesin Motor Proteins by Tau by Dixit, Ram, Ross, Jennifer L, Goldman, Yale E, Holzbaur, Erika L.F

“…Dynein and kinesin motor proteins transport cellular cargoes toward opposite ends of microtubule tracks. In neurons, microtubules are abundantly decorated with…”
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Single-Molecule Fluorescence Measurements of Ribosomal Translocation Dynamics by Chen, Chunlai, Stevens, Benjamin, Kaur, Jaskarin, Cabral, Diana, Liu, Hanqing, Wang, Yuhong, Zhang, Haibo, Rosenblum, Gabriel, Smilansky, Zeev, Goldman, Yale E., Cooperman, Barry S.

“…We employ single-molecule fluorescence resonance energy transfer (smFRET) to study structural dynamics over the first two elongation cycles of protein…”
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Dynein Tethers and Stabilizes Dynamic Microtubule Plus Ends by Hendricks, Adam G., Lazarus, Jacob E., Perlson, Eran, Gardner, Melissa K., Odde, David J., Goldman, Yale E., Holzbaur, Erika L.F.

“…Microtubules undergo alternating periods of growth and shortening, known as dynamic instability. These dynamics allow microtubule plus ends to explore cellular…”
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Ataluren binds to multiple protein synthesis apparatus sites and competitively inhibits release factor-dependent termination by Huang, Shijie, Bhattacharya, Arpan, Ghelfi, Mikel D., Li, Hong, Fritsch, Clark, Chenoweth, David M., Goldman, Yale E., Cooperman, Barry S.

“…Genetic diseases are often caused by nonsense mutations, but only one TRID (translation readthrough inducing drug), ataluren, has been approved for clinical…”
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Specific catalytically impaired DDX3X mutants form sexually dimorphic hollow condensates by Owens, Michael C., Shen, Hui, Yanas, Amber, Mendoza-Figueroa, Maria Saraí, Lavorando, Ellen, Wei, Xiaoyu, Shweta, Him, Tang, Hsin-Yao, Goldman, Yale E., Liu, Kathy Fange

“…Mutations in the RNA helicase DDX3X, implicated in various cancers and neurodevelopmental disorders, often impair RNA unwinding and translation. However, the…”
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Tilting and Wobble of Myosin V by High-Speed Single-Molecule Polarized Fluorescence Microscopy by Beausang, John F., Shroder, Deborah Y., Nelson, Philip C., Goldman, Yale E.

“…Myosin V is biomolecular motor with two actin-binding domains (heads) that take multiple steps along actin by a hand-over-hand mechanism. We used high-speed…”
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Single molecule mechanics resolves the earliest events in force generation by cardiac myosin by Woody, Michael S, Winkelmann, Donald A, Capitanio, Marco, Ostap, E Michael, Goldman, Yale E

“…Key steps of cardiac mechanochemistry, including the force-generating working stroke and the release of phosphate (P ), occur rapidly after myosin-actin…”
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Dynamics of translation by single ribosomes through mRNA secondary structures by Chen, Chunlai, Zhang, Haibo, Broitman, Steven L, Reiche, Michael, Farrell, Ian, Cooperman, Barry S, Goldman, Yale E

“…The secondary structure of mRNAs can slow or even halt protein synthesis. Single-molecule FRET studies of distinct steps in the translation elongation cycle…”
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Exo70 Stimulates the Arp2/3 Complex for Lamellipodia Formation and Directional Cell Migration by Liu, Jianglan, Zhao, Yuting, Sun, Yujie, He, Bing, Yang, Changsong, Svitkina, Tatyana, Goldman, Yale E., Guo, Wei

“…Directional cell migration requires the coordination of actin assembly and membrane remodeling. The exocyst is an octameric protein complex essential for…”
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Nanoaperture fabrication via colloidal lithography for single molecule fluorescence analysis by Jamiolkowski, Ryan M, Chen, Kevin Y, Fiorenza, Shane A, Tate, Alyssa M, Pfeil, Shawn H, Goldman, Yale E

“…In single molecule fluorescence studies, background emission from labeled substrates often restricts their concentrations to non-physiological nanomolar…”
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Myosin with hypertrophic cardiac mutation R712L has a decreased working stroke which is rescued by omecamtiv mecarbil by Snoberger, Aaron, Barua, Bipasha, Atherton, Jennifer L, Shuman, Henry, Forgacs, Eva, Goldman, Yale E, Winkelmann, Donald A, Ostap, E Michael

“…Hypertrophic cardiomyopathies (HCMs) are the leading cause of acute cardiac failure in young individuals. Over 300 mutations throughout β-cardiac myosin,…”
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Force-Dependent Detachment of Kinesin-2 Biases Track Switching at Cytoskeletal Filament Intersections by Schroeder, Harry W., Hendricks, Adam G., Ikeda, Kazuho, Shuman, Henry, Rodionov, Vladimir, Ikebe, Mitsuo, Goldman, Yale E., Holzbaur, Erika L.F.

“…Intracellular trafficking of organelles often involves cytoskeletal track switching. Organelles such as melanosomes are transported by multiple motors…”
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Straightening Out the Elasticity of Myosin Cross-Bridges by Linari, Marco, Piazzesi, Gabriella, Pertici, Irene, Dantzig, Jody A., Goldman, Yale E., Lombardi, Vincenzo

“…In a contracting muscle, myosin cross-bridges extending from thick filaments pull the interdigitating thin (actin-containing) filaments during cyclical…”
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MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation by Woody, Michael S., Lewis, John H., Greenberg, Michael J., Goldman, Yale E., Ostap, E. Michael

“…We present MEMLET (MATLAB-enabled maximum-likelihood estimation tool), a simple-to-use and powerful program for utilizing maximum-likelihood estimation (MLE)…”
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Myosin IC generates power over a range of loads via a new tension-sensing mechanism by Greenberg, Michael J, Lin, Tianming, Goldman, Yale E, Shuman, Henry, Ostap, E. Michael

“…Myosin IC (myo1c), a widely expressed motor protein that links the actin cytoskeleton to cell membranes, has been associated with numerous cellular processes,…”
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Coordination of molecular motors: from in vitro assays to intracellular dynamics by Holzbaur, Erika LF, Goldman, Yale E

“…New technologies have emerged that enable the tracking of molecular motors and their cargos with very high resolution both in vitro and in live cells. Classic…”
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Angular measurements of the dynein ring reveal a stepping mechanism dependent on a flexible stalk by Lippert, Lisa G., Dadosh, Tali, Hadden, Jodi A., Karnawat, Vishakha, Diroll, Benjamin T., Murray, Christopher B., Holzbaur, Erika L. F., Schulten, Klaus, Reck-Peterson, Samara L., Goldman, Yale E.

“…The force-generating mechanism of dynein differs from the force-generating mechanisms of other cytoskeletal motors. To examine the structural dynamics of…”
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