Search Results - "Goldberg, Gregory W."

Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity by Samai, Poulami, Pyenson, Nora, Jiang, Wenyan, Goldberg, Gregory W., Hatoum-Aslan, Asma, Marraffini, Luciano A.

“…Immune systems must recognize and destroy different pathogens that threaten the host. CRISPR-Cas immune systems protect prokaryotes from viral and plasmid…”
Get full text

Conditional tolerance of temperate phages via transcription-dependent CRISPR-Cas targeting by Goldberg, Gregory W., Jiang, Wenyan, Bikard, David, Marraffini, Luciano A.

“…The Staphylococcus epidermidis CRISPR-Cas system can prevent lytic infection but tolerate lysogenization by temperate phage through a transcription-dependent…”
Get full text

Incomplete prophage tolerance by type III-A CRISPR-Cas systems reduces the fitness of lysogenic hosts by Goldberg, Gregory W., McMillan, Elizabeth A., Varble, Andrew, Modell, Joshua W., Samai, Poulami, Jiang, Wenyan, Marraffini, Luciano A.

“…CRISPR–Cas systems offer an immune mechanism through which prokaryotic hosts can acquire heritable resistance to genetic parasites, including temperate phages…”
Get full text

Engineered dual selection for directed evolution of SpCas9 PAM specificity by Goldberg, Gregory W., Spencer, Jeffrey M., Giganti, David O., Camellato, Brendan R., Agmon, Neta, Ichikawa, David M., Boeke, Jef D., Noyes, Marcus B.

“…The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA targeting specificity from protein-DNA contacts with protospacer adjacent motif…”
Get full text

Regional Modulation of a Stochastically Expressed Factor Determines Photoreceptor Subtypes in the Drosophila Retina by Thanawala, Shivani U., Rister, Jens, Goldberg, Gregory W., Zuskov, Andrey, Olesnicky, Eugenia C., Flowers, Jonathan M., Jukam, David, Purugganan, Michael D., Gavis, Elizabeth R., Desplan, Claude, Johnston, Robert J.

“…Stochastic mechanisms are sometimes utilized to diversify cell fates, especially in nervous systems. In the Drosophila retina, stochastic expression of the…”
Get full text

Cas9 specifies functional viral targets during CRISPR–Cas adaptation by Heler, Robert, Samai, Poulami, Modell, Joshua W., Weiner, Catherine, Goldberg, Gregory W., Bikard, David, Marraffini, Luciano A.

“…Clustered regularly interspaced short palindromic repeat (CRISPR) loci and their associated (Cas) proteins provide adaptive immunity against viral infection in…”
Get full text

Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials by Bikard, David, Euler, Chad W, Jiang, Wenyan, Nussenzweig, Philip M, Goldberg, Gregory W, Duportet, Xavier, Fischetti, Vincent A, Marraffini, Luciano A

“…Coupling the specificity of CRISPR-Cas nucleases and bacteriophage delivery enables exquisitely precise bacterial killing. Antibiotics target conserved…”
Get full text

Resistance and tolerance to foreign elements by prokaryotic immune systems — curating the genome by Goldberg, Gregory W., Marraffini, Luciano A.

“…This Essay considers how prokaryotic and mammalian immune systems ensure tolerance or resistance to genetic elements. In particular, the authors discuss the…”
Get full text

A universal deep-learning model for zinc finger design enables transcription factor reprogramming by Ichikawa, David M., Abdin, Osama, Alerasool, Nader, Kogenaru, Manjunatha, Mueller, April L., Wen, Han, Giganti, David O., Goldberg, Gregory W., Adams, Samantha, Spencer, Jeffrey M., Razavi, Rozita, Nim, Satra, Zheng, Hong, Gionco, Courtney, Clark, Finnegan T., Strokach, Alexey, Hughes, Timothy R., Lionnet, Timothee, Taipale, Mikko, Kim, Philip M., Noyes, Marcus B.

“…Cys 2 His 2 zinc finger (ZF) domains engineered to bind specific target sequences in the genome provide an effective strategy for programmable regulation of…”
Get full text

Resistance and tolerance of foreign genetic elements by prokaryotic immune systems — curating the genome by Goldberg, Gregory W., Marraffini, Luciano A.

“…In order to engage in adaptive symbioses or genetic exchange, organisms must interact with foreign, non-self elements despite the risks of predation and…”
Get full text

Development of sequence-specific antimicrobials based on programmable CRISPR-Cas nucleases by Bikard, David, Euler, Chad, Jiang, Wenyan, Nussenzweig, Philip M., Goldberg, Gregory W., Duportet, Xavier, Fischetti, Vincent A., Marraffini, Luciano A.

“…Antibiotics target conserved bacterial cellular pathways or growth functions and therefore cannot selectively kill specific members of a complex microbial…”
Get full text