Search Results - "Gokarn, Yatin"

Weak Interactions Govern the Viscosity of Concentrated Antibody Solutions: High-Throughput Analysis Using the Diffusion Interaction Parameter by Connolly, Brian D., Petry, Chris, Yadav, Sandeep, Demeule, Barthélemy, Ciaccio, Natalie, Moore, Jamie M.R., Shire, Steven J., Gokarn, Yatin R.

“…Weak protein-protein interactions are thought to modulate the viscoelastic properties of concentrated antibody solutions. Predicting the viscoelastic behavior…”
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Particle shape enhances specificity of antibody-displaying nanoparticles by Barua, Sutapa, Yoo, Jin-Wook, Kolhar, Poornima, Wakankar, Aditya, Gokarn, Yatin R., Mitragotri, Samir

“…Monoclonal antibodies are used in numerous therapeutic and diagnostic applications; however, their efficacy is contingent on specificity and avidity. Here, we…”
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Designing Robust Monoclonal Antibody Drug Products: Pitfalls of Simplistic Approaches for Stability Prediction by Rembert, Kelvin B, Gokarn, Yatin R, Saluja, Atul

“…Thermal stability attributes including unfolding onset (T ) and mid-point (T ) are often utilized for efficient development of monoclonal antibody (mAb)…”
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Analytical methods for physicochemical characterization of antibody drug conjugates by Wakankar, Aditya, Chen, Yan, Gokarn, Yatin, Jacobson, Fredric S.

“…Antibody-drug conjugates (ADCs), formed through the chemical linkage of a potent small molecule cytotoxin (drug) to a monoclonal antibody, have more complex…”
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A single molecular descriptor to predict solution behavior of therapeutic antibodies by Kingsbury, Jonathan S, Saini, Amandeep, Auclair, Sarah Marie, Fu, Li, Lantz, Michaela M, Halloran, Kevin T, Calero-Rubio, Cesar, Schwenger, Walter, Airiau, Christian Y, Zhang, Jifeng, Gokarn, Yatin R

“…Despite the therapeutic success of monoclonal antibodies (mAbs), early identification of developable mAb drug candidates with optimal manufacturability,…”
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Diffusion and Sedimentation Interaction Parameters for Measuring the Second Virial Coefficient and Their Utility as Predictors of Protein Aggregation by Saluja, Atul, Fesinmeyer, R. Matthew, Hogan, Sabine, Brems, David N., Gokarn, Yatin R.

“…The concentration-dependence of the diffusion and sedimentation coefficients ( k D and k s , respectively) of a protein can be used to determine the second…”
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Characterization of Opalescence in low Volume Monoclonal Antibody Solutions Enabled by Microscale Nephelometry by Kingsbury, Jonathan S, Lantz, Michaela M, Saini, Amandeep, Wang, Michael Z, Gokarn, Yatin R

“…Monoclonal antibody (mAb)-based drugs are often prone to unfavorable solution behaviors including high viscosity, opalescence, phase separation, and…”
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Ultradilute Measurements of Self-Association for the Identification of Antibodies with Favorable High-Concentration Solution Properties by Starr, Charles G, Makowski, Emily K, Wu, Lina, Berg, Brendan, Kingsbury, Jonathan S, Gokarn, Yatin R, Tessier, Peter M

“…There is significant interest in formulating antibody therapeutics as concentrated liquid solutions, but early identification of developable antibodies with…”
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Effect of PEGylation on Protein Hydrodynamics by Gokarn, Yatin R, McLean, Matthew, Laue, Thomas M

“…We studied the effect of PEGylation on protein hydrodynamic behavior using hen egg-white lysozyme (HEWL) as a model protein. HEWL was PEGylated with a linear,…”
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Small-Angle Neutron Scattering Characterization of Monoclonal Antibody Conformations and Interactions at High Concentrations by Yearley, Eric J., Zarraga, Isidro E., Shire, Steven J., Scherer, Thomas M., Gokarn, Yatin, Wagner, Norman J., Liu, Yun

“…Small-angle neutron scattering (SANS) is used to probe the solution structure of two protein therapeutics (monoclonal antibodies 1 and 2 (MAb1 and MAb2)) and…”
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Effective charge measurements reveal selective and preferential accumulation of anions, but not cations, at the protein surface in dilute salt solutions by Gokarn, Yatin R., Fesinmeyer, R.Matthew, Saluja, Atul, Razinkov, Vladimir, Chase, Susan F., Laue, Thomas M., Brems, David N.

“…Specific‐ion effects are ubiquitous in nature; however, their underlying mechanisms remain elusive. Although Hofmeister‐ion effects on proteins are observed at…”
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Characterization of antibody aggregation: role of buried, unpaired cysteines in particle formation by Brych, Stephen R, Gokarn, Yatin R, Hultgen, Heather, Stevenson, Riki J, Rajan, Rahul, Matsumura, Masazumi

“…Proteins are susceptible to degradation upon exposure to a variety of stresses during product manufacturing, transportation and storage. In this study, we…”
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Effect of Ions on Agitation- and Temperature-Induced Aggregation Reactions of Antibodies by Fesinmeyer, R. Matthew, Hogan, Sabine, Saluja, Atul, Brych, Stephen R, Kras, Eva, Narhi, Linda O, Brems, David N, Gokarn, Yatin R

“…Purpose The impact of ions on protein aggregation remains poorly understood. We explored the role of ionic strength and ion identity on the temperature- and…”
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A biomimetic chip to assess subcutaneous bioavailability of monoclonal antibodies in humans by Chandran Suja, Vineeth, Qi, Qin M, Halloran, Kevin, Zhang, Jifeng, Shaha, Suyog, Prakash, Supriya, Kumbhojkar, Ninad, Deslandes, Antoine, Huille, Sylvain, Gokarn, Yatin R, Mitragotri, Samir

“…Abstract Subcutaneous (subQ) injection is a common route for delivering biotherapeutics, wherein pharmacokinetics is largely influenced by drug transport in a…”
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Polar solvents decrease the viscosity of high concentration IgG1 solutions through hydrophobic solvation and interaction: formulation and biocompatibility considerations by Kamerzell, Tim J, Pace, Amanda L, Li, Megan, Danilenko, Dimitry M, McDowell, Michelle, Gokarn, Yatin R, Wang, Y John

“…Low-volume protein dosage forms for subcutaneous injection pose unique challenges to the pharmaceutical scientist. Indeed, high protein concentrations are…”
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Ion‐specific modulation of protein interactions: Anion‐induced, reversible oligomerization of a fusion protein by Gokarn, Yatin R., Fesinmeyer, R. Matthew, Saluja, Atul, Cao, Shawn, Dankberg, Jane, Goetze, Andrew, Jr, Richard L. Remmele, Narhi, Linda O., Brems, David N.

“…Ions can significantly modulate the solution interactions of proteins. We aim to demonstrate that the salt‐dependent reversible heptamerization of a fusion…”
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Self-buffering antibody formulations by Gokarn, Yatin R, Kras, Eva, Nodgaard, Carrie, Dharmavaram, Vasumathi, Fesinmeyer, R Matthew, Hultgen, Heather, Brych, Stephen, Remmele, Jr, Richard L, Brems, David N, Hershenson, Susan

“…Monoclonal antibodies (mAbs) often require the development of high-concentration formulations. In such cases, and when it is desirable to formulate a mAb…”
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Anion Binding Mediated Precipitation of a Peptibody by Saluja, Atul, Crampton, Shon, Kras, Eva, Fesinmeyer, R. Matthew, Remmele, Richard L. Jr, Narhi, Linda O, Brems, David N, Gokarn, Yatin R

“…Purpose Understand the underlying mechanism governing the salt-induced precipitation of a basic (pI = 8.8) protein, Peptibody A (PbA), in acidic solutions…”
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Non-invasive delivery strategies for biologics by Anselmo, Aaron C., Gokarn, Yatin, Mitragotri, Samir

“…The requirement for delivery by injection is currently a limitation for the use of biologic drugs such as antibodies. In this Review, Mitragotri and colleagues…”
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Machine Learning Applied to Determine the Molecular Descriptors Responsible for the Viscosity Behavior of Concentrated Therapeutic Antibodies by Lai, Pin-Kuang, Fernando, Amendra, Cloutier, Theresa K, Gokarn, Yatin, Zhang, Jifeng, Schwenger, Walter, Chari, Ravi, Calero-Rubio, Cesar, Trout, Bernhardt L

“…Predicting the solution viscosity of monoclonal antibody (mAb) drug products remains as one of the main challenges in antibody drug design, manufacturing, and…”
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