Catheter‐based induction of renal ischemia/reperfusion in swine: description of an experimental model

Catheter‐based induction of renal ischemia/reperfusion in swine: description of an experimental model

Several techniques to induce renal ischemia have been proposed: clamp, PVA particles, and catheter‐balloon. We report the development of a controlled, single‐insult model of unilateral renal ischemia/reperfusion (I/R) without contralateral nephrectomy, using a suitable model, the pig. This is a ball...

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Published in:Physiological reports Vol. 2; no. 9; pp. e12150 - n/a
Main Authors: Malagrino, Pamella A., Venturini, Gabriela, Yogi, Patrícia S., Dariolli, Rafael, Padilha, Kallyandra, Kiers, Bianca, Gois, Tamiris C., Motta‐Leal‐Filho, Joaquim M., Takimura, Celso K., Girardi, Adriana C. C., Carnevale, Francisco C., Zeri, Ana C. M., Malheiros, Denise M. A. C., Krieger, José E., Pereira, Alexandre C.
Format: Journal Article
Language:English
Published: United States John Wiley & Sons, Inc 01-09-2014
Wiley Periodicals, Inc
Subjects:
Acute kidney injury
acute tubular necrosis
Anesthesia
Angioplasty
Animals
Balloon catheters
balloon‐catheter
Bicarbonates
Biomarkers
Catheters
Clinical medicine
Creatinine
Dissection
Excretion
Gel electrophoresis
Gelatinase
Glucose
Heart surgery
Hogs
Ischemia
Kidneys
Lipocalin
Medical imaging
Medical research
Nephrectomy
NMR
Nuclear magnetic resonance
Original Research
Oxidative stress
Physiology
Potassium
Renal artery
renal artery occlusion
Renal function
Reperfusion
Sodium
Sodium lauryl sulfate
Structure-function relationships
swine model
Urea
Urine
Veins & arteries
Brazil
swine model
acute tubular necrosis
balloon‐catheter
Acute kidney injury
ischemia
renal artery occlusion
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Summary:Several techniques to induce renal ischemia have been proposed: clamp, PVA particles, and catheter‐balloon. We report the development of a controlled, single‐insult model of unilateral renal ischemia/reperfusion (I/R) without contralateral nephrectomy, using a suitable model, the pig. This is a balloon‐catheter‐based model using a percutaneous, interventional radiology procedure. One angioplasty balloon‐catheter was placed into the right renal artery and inflated for 120 min and reperfusion over 24 h. Serial serums were sampled from the inferior vena cava and urine was directly sampled from the bladder throughout the experiment, and both kidneys were excised after 24 h of reperfusion. Analyses of renal structure and function were performed by hematoxylin–eosin/periodic Acid‐Schiff, serum creatinine (SCr), blood urea nitrogen (BUN), fractional excretion of ions, and glucose, SDS‐PAGE analysis of urinary proteins, and serum neutrophil gelatinase‐associated lipocalin (NGAL). Total nitrated protein was quantified to characterize oxidative stress. Acute tubular necrosis (ATN) was identified in every animal, but only two animals showed levels of SCr above 150% of baseline values. As expected, I/R increased SCr and BUN. Fractional sodium, potassium, chloride, and bicarbonate excretion were modulated during ischemia. Serum‐nitrated proteins and NGAL had two profiles: decreased with ischemia and increased after reperfusion. This decline was associated with increased protein excretion during ischemia and early reperfusion. Altogether, these data show that the renal I/R model can be performed by percutaneous approach in the swine model. This is a suitable translational model to study new early renal ischemic biomarkers and pathophysiological mechanisms in renal ischemia. e12150 We report the development of a controlled, single‐insult model of unilateral renal ischemia/reperfusion (I/R) without contralateral nephrectomy, using a suitable model, the pig. This model can be useful for translational studies, including development of new diagnosis biomarkers and drugs studies. Furthermore, this kind of data is of paramount importance to better understand the pathophysiological mechanisms of renal disease.
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ISSN:2051-817X
2051-817X
DOI:10.14814/phy2.12150