Search Results - "Goh, Lin Tang"

Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells by Haryadi, Ryan, Ho, Steven, Kok, Yee Jiun, Pu, Helen X, Zheng, Lu, Pereira, Natasha A, Li, Bin, Bi, Xuezhi, Goh, Lin-Tang, Yang, Yuansheng, Song, Zhiwei

“…Translocation of a nascent protein from the cytosol into the ER mediated by its signal peptide is a critical step in protein secretion. The aim of this work…”
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Comparison of internal ribosome entry site (IRES) and Furin-2A (F2A) for monoclonal antibody expression level and quality in CHO cells by Ho, Steven C L, Bardor, Muriel, Li, Bin, Lee, Jia Juan, Song, Zhiwei, Tong, Yen Wah, Goh, Lin-Tang, Yang, Yuansheng

“…Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC), and dihydrofolate reductase (DHFR) in one transcript were…”
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Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures by Chee Furng Wong, Danny, Tin Kam Wong, Kathy, Tang Goh, Lin, Kiat Heng, Chew, Gek Sim Yap, Miranda

“…As we pursue the means to improve yields to meet growing therapy demands, it is important to examine the impact of process control on glycosylation patterns to…”
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Production of functional soluble Dectin-1 glycoprotein using an IRES-linked destabilized-dihydrofolate reductase expression vector by Ng, Say Kong, Tan, Tessa Rui Min, Wang, Yang, Ng, Daniel, Goh, Lin-Tang, Bardor, Muriel, Wong, Victor Vai Tak, Lam, Kong Peng

“…Dectin-1 (CLEC7A) is a C-type lectin receptor that binds to β-glucans found in fungal cell walls to act as a major pattern recognition receptor (PRR). Since…”
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Crystallization of IgG1 by mapping its liquid-liquid phase separation curves by Jion, Adam Idu, Goh, Lin-Tang, Oh, Steve K.W.

“…Monoclonal antibody therapeutics is an important and fast expanding market. While production of these molecules has been a major area of research, much less is…”
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GlycoVis: Visualizing glycan distribution in the protein N-glycosylation pathway in mammalian cells by Hossler, Patrick, Goh, Lin-Tang, Lee, May May, Hu, Wei-Shou

“…Glycosylation has profound effects on the quality of recombinant proteins produced in mammalian cells. The biosynthetic pathways of N‐linked glycans on…”
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Identification of Autocrine Growth Factors Secreted by CHO Cells for Applications in Single-Cell Cloning Media by Lim, U Ming, Yap, Miranda Gek Sim, Lim, Yoon Pin, Goh, Lin-Tang, Ng, Say Kong

“…Chinese hamster ovary (CHO) cell lines are widely used for the expression of therapeutic recombinant proteins, including monoclonal antibodies and other…”
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Determination of interferon-gamma in Chinese hamster ovary cell culture supernatant by coupled-column liquid chromatography by Goh, Lin-Tang, Yap, Miranda Gek Sim

“…A robust tandem HPLC method coupling size‐exclusion (Shodex Asahipak GS‐320HQ) and reversed phase (Vydac 218TP54) columns with ultraviolet detection was…”
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Rapid quantification of Escherichia coli in food and media using bacteriophage T7 amplification and liquid chromatography-multiple reaction monitoring tandem mass spectrometry by Banu, Mazlina, Ng, Daniel, Zheng, Lu, Goh, Lin-Tang, Bi, Xuezhi, Ow, Dave Siak-Wei

“…•Specific detection of Escherichia coli using heavy isotope-labeled bacteriophage T7.•T7 infects E. coli and amplifies their copy numbers nearly a 100-fold in…”
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Metabolomics profiling of extracellular metabolites in recombinant Chinese Hamster Ovary fed-batch culture by Chong, William P. K., Goh, Lin Tang, Reddy, Satty G., Yusufi, Faraaz N.K., Lee, Dong Yup, Wong, Niki S. C., Heng, Chew Kiat, Yap, Miranda G. S., Ho, Ying Swan

“…A metabolomics‐based approach was used to time profile extracellular metabolites in duplicate fed‐batch bioreactor cultures of recombinant Chinese Hamster…”
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RNA interference of sialidase improves glycoprotein sialic acid content consistency by Ngantung, Frederyk A., Miller, Peter G., Brushett, Fikile R., Tang, Goh Lin, Wang, Daniel I.C.

“…An important challenge facing therapeutic protein production in mammalian cell culture is the cleavage of terminal sialic acids on recombinant protein glycans…”
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Identification of proteins from feeder conditioned medium that support human embryonic stem cells by Chin, Angela Chui Ping, Fong, Wey Jia, Goh, Lin-Tang, Philp, Robin, Oh, Steve Kah Weng, Choo, Andre Boon Hwa

“…The maintenance of undifferentiated human embryonic stem cells (hESC) requires feeder cells, either in co-culture or feeder-free with conditioned medium (CM)…”
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Pharmaceutical metabolite profiling using quadrupole/ion mobility spectrometry/time-of-flight mass spectrometry by Chan, Eric C. Y., New, Lee Sun, Yap, Chun Wei, Goh, Lin Tang

“…The use of hybrid quadrupole ion mobility spectrometry time‐of‐flight mass spectrometry (Q/IMS/TOFMS) in the metabolite profiling of leflunomide (LEF) and…”
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Identification of Stereoisomeric Metabolites of Meisoindigo in Rat Liver Microsomes by Achiral and Chiral Liquid Chromatography/Tandem Mass Spectrometry by MENG HUANG, LIN TANG GOH, HO, Paul C

“…N -Methylisoindigotin, abbreviated as meisoindigo, has been a routine therapeutic agent in the clinical treatment of chronic myelogenous leukemia in China…”
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Effect of pH, agitation and aeration on hyaluronic acid production by Streptococcus zooepidemicus by JOHNS, M. R, LIN-TANG GOH, OEGGERLI, A

“…The optimum pH for both the rate of production and yield of hyaluronic acid (HA) by Streptococcus zooepidemicus from glucose medium was 6.7 plus or minus 0.2…”
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Production and purification of recombinant Blomia tropicalis group 5 allergen from Pichia pastoris culture by GOH, Lin-Tang, KUO, I-Chun, SA LUO, KAW YAN CHUA, WHITE, Moshe

“…The gene of Blomia tropicalis group 5 allergen (Blo t 5) was cloned and expressed in Pichia pastoris KM71. Selected KM71 clones were cultivated in a fed-batch…”
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Effect of pH, agitation and aeration on hyaluronic acid production byStreptococcus zooepidemicus by Johns, Michael R., Goh, Lin-Tang, Oeggerli, Andreas

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Comparison of Internal Ribosome Entry Site by Ho, Steven C. L, Bardor, Muriel, Li, Bin, Lee, Jia Juan, Song, Zhiwei, Tong, Yen Wah, Goh, Lin-Tang, Yang, Yuansheng

“…Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC), and dihydrofolate reductase (DHFR) in one transcript were…”
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Comparison of Internal Ribosome Entry Site for Monoclonal Antibody Expression Level and Quality in CHO Cells by Ho, Steven C. L, Bardor, Muriel, Li, Bin, Lee, Jia Juan, Song, Zhiwei, Tong, Yen Wah, Goh, Lin-Tang, Yang, Yuansheng

“…Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC), and dihydrofolate reductase (DHFR) in one transcript were…”
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Crystallization of IgG 1 by mapping its liquid–liquid phase separation curves by Jion, Adam Idu, Goh, Lin‐Tang, Oh, Steve K.W.

“…Monoclonal antibody therapeutics is an important and fast expanding market. While production of these molecules has been a major area of research, much less is…”
Get full text