Orthotopic tumorgrafts in nude mice as a model to evaluate calcitriol effects in breast cancer/Modelo de enxerto tumoral ortotopico em camundongos para avaliar os efeitos do calcitriol em cancer de mama

Orthotopic tumorgrafts in nude mice as a model to evaluate calcitriol effects in breast cancer/Modelo de enxerto tumoral ortotopico em camundongos para avaliar os efeitos do calcitriol em cancer de mama

Calcitriol antiproliferative effects were observed in xenografts of breast cancer cell lines, however they were not yet investigated in tumorgrafts, consisting of freshly collected breast cancer samples xenografted into animals. Objectives: To establish a tumorgraft model, from freshly collected bre...

Full description

Saved in:
Bibliographic Details
Published in:Brazilian journal of biology Vol. 77; no. 4; pp. 856 - 867
Main Authors: Fonseca-Filho, V.C.N, Katayama, M.L.H, Lyra, E.C, Maria, D.A, Basso, R.A, Nonogaki, S, Guerra, J.M, Maistro, S, Goes, J.C.G.S, Folgueira, M.A.A.K
Format: Journal Article
Language:English
Published: Association of the Brazilian Journal of Biology 01-11-2017
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Calcitriol antiproliferative effects were observed in xenografts of breast cancer cell lines, however they were not yet investigated in tumorgrafts, consisting of freshly collected breast cancer samples xenografted into animals. Objectives: To establish a tumorgraft model, from freshly collected breast cancer samples, which were directly implanted in nude mice, to study calcitriol effects. Methods: Breast cancer samples collected from 12 patients were orthotopically implanted into nude mice. Animals were treated with weekly intratumoral injections of calcitriol 3 [micro]g/Kg, which was previously shown to induce peak serum calcitriol levels in the predicted therapeutic range. Results: Success engraftment rate was 25%. Tumorgrafts were established from aggressive (HER2 positive or histological grade 3) highly proliferative samples and original tumor characteristics were preserved. Calcitriol highly induced its target gene, CYP24A1, indicating that the genomic vitamin D pathway is active in tumorgrafts. However, no differences in the expression of proliferation and apoptosis markers (BrdU incorporation, Ki67, CDKN1A, CDKN1B, BCL2 expression) were observed in these highly proliferative tumor samples. Conclusions: Tumorgrafts seem a promising model to explore other calcitriol doses and regimens, considering the heterogeneity of the disease and microenvironment interactions.
ISSN:1519-6984
1678-4375
DOI:10.1590/1519-6984.04016