The effect of β-ionone on bacterial cells: the use of specific lux-biosensors

The diversity of the biological activity of volatile organic compounds (VOCs), including unsaturated ketone β-ionone, promising pharmacological, biotechnological, and agricultural agent, has aroused considerable interest. However, the functional role and mechanisms of action of VOCs remain insuffici...

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Bibliographic Details
Published in:	Research in microbiology Vol. 175; no. 7; p. 104214
Main Authors:	Plyuta, Vladimir A., Sidorova, Daria E., Koksharova, Olga A., Khmel, Inessa A., Gnuchikh, Evgeniy Y., Melkina, Olga E.
Format:	Journal Article
Language:	English
Published:	France Elsevier Masson SAS 01-09-2024
Subjects:	Bacterial stress response Bioluminescence Lux-biosensors Unsaturated ketone β-ionone Volatile organic compound Lux-biosensors Bioluminescence Unsaturated ketone β-ionone Volatile organic compound Bacterial stress response
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Summary:	The diversity of the biological activity of volatile organic compounds (VOCs), including unsaturated ketone β-ionone, promising pharmacological, biotechnological, and agricultural agent, has aroused considerable interest. However, the functional role and mechanisms of action of VOCs remain insufficiently studied. In this work, the response of bacterial cells to the action of β-ionone was studied using specific bioluminescent lux-biosensors containing stress-sensitive promoters. We determined that in Escherichia coli cells, β-ionone induces oxidative stress (PkatG and Pdps promoters) through a specific response mediated by the OxyR/OxyS regulon, but not SoxR/SoxS (PsoxS promoter). It has been shown that β-ionone at high concentrations (50 μM and above) causes a weak induction of the expression from the PibpA promoter and slightly induces the PcolD promoter in the E. coli biosensors; the observed effect is enhanced in the ΔoxyR mutants. This indicates the presence of some damage to proteins and DNA. β-Ionone was found to inhibit the bichaperone-dependent DnaKJE-ClpB refolding of heat-inactivated bacterial luciferase in E. coli wild-type and ΔibpB mutant strains. In the cells of the Gram-positive bacterium Bacillus subtilis 168 pNK-MrgA β-ionone does not cause oxidative stress. Thus, in this work, the specificity of bacterial cell stress responses to the action of β-ionone was shown.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0923-2508 1769-7123 1769-7123
DOI:	10.1016/j.resmic.2024.104214