Search Results - "Gmeiner, Christoph"
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Integrative solution structure of PTBP1-IRES complex reveals strong compaction and ordering with residual conformational flexibility
Published in Nature communications (13-10-2023)“…RNA-binding proteins (RBPs) are crucial regulators of gene expression, often composed of defined domains interspersed with flexible, intrinsically disordered…”
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Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris
Published in Scientific reports (20-11-2013)“…The yeast Pichia pastoris is a common host for the recombinant production of biopharmaceuticals, capable of performing posttranslational modifications like…”
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3
Resolving distance variations by single-molecule FRET and EPR spectroscopy using rotamer libraries
Published in Biophysical journal (02-11-2021)“…Förster resonance energy transfer (FRET) and electron paramagnetic resonance (EPR) spectroscopy are complementary techniques for quantifying distances in the…”
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Effects of different media supplements on the production of an active recombinant plant peroxidase in a Pichia pastoris Δoch1 strain
Published in Bioengineered (2015)“…Recombinant protein production in microorganisms is one of the most studied areas of research in biotechnology today. In this respect the yeast Pichia pastoris…”
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Protein Production with a Pichia pastoris OCH1 Knockout Strain in Fed-Batch Mode
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2015)“…The methylotrophic yeast Pichia pastoris is a widely used host organism for recombinant protein production in biotechnology and pharmaceutical industry…”
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Recombinant horseradish peroxidase variants for targeted cancer treatment
Published in Cancer medicine (Malden, MA) (01-06-2016)“…Cancer is a major cause of death. Common chemo‐ and radiation‐therapies damage healthy tissue and cause painful side effects. The enzyme horseradish peroxidase…”
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Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase
Published in Microbial cell factories (08-01-2015)“…Pichia pastoris is a prominent host for recombinant protein production, amongst other things due to its capability of glycosylation. However, N-linked glycans…”
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Orthogonal Tyrosine and Cysteine Site-Directed Spin Labeling for Dipolar Pulse EPR Spectroscopy on Proteins
Published in The journal of physical chemistry letters (05-10-2017)“…Site-directed spin labeling of native tyrosine residues in isolated domains of the protein PTBP1, using a Mannich-type reaction, was combined with conventional…”
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Two-Dimensional Distance Correlation Maps from Pulsed Triple Electron Resonance (TRIER) on Proteins with Three Paramagnetic Centers
Published in Applied magnetic resonance (01-11-2018)“…Recently, we introduced the pulsed triple electron resonance (TRIER) experiment, which correlates dipolar frequencies of molecules with three electron spins…”
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10
Development of a fed-batch process for a recombinant Pichia pastoris [DELA]och1 strain expressing a plant peroxidase
Published in Microbial cell factories (08-01-2015)“…Pichia pastoris is a prominent host for recombinant protein production, amongst other things due to its capability of glycosylation. However, N-linked glycans…”
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