Genetic segregation of microsatellite markers in Saccharum officinarum and S. spontaneum

Genetic segregation of microsatellite markers in Saccharum officinarum and S. spontaneum

Genetic mapping techniques can be used to study the interaction between two different genomes after hybridization. This study investigated a Saccharum officinarum (Green German or GG, 2n11x110) S. spontaneum (IND 81-146 or IND, 2n7x56) interspecific cross. Segregation of 193 microsatellite (SSR) loc...

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Bibliographic Details
Published in:Heredity Vol. 97; no. 5; pp. 366 - 375
Main Authors: Edmé, S.J, Glynn, N.G, Comstock, J.C
Format: Journal Article
Language:English
Published: England Springer Nature B.V 01-11-2006
Subjects:
Chromosome Mapping
DNA, Plant - genetics
Flowers & plants
Gene loci
gene segregation
Genetic markers
Genetics
Genome, Plant
Genomics
Hybridization
Hybridization, Genetic
hybrids
interspecific hybridization
linkage groups
loci
Microsatellite Repeats
Pollination
Saccharum - classification
Saccharum - genetics
Saccharum officinarum
Saccharum spontaneum
segregation distortion
Species Specificity
sugarcane
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Summary:Genetic mapping techniques can be used to study the interaction between two different genomes after hybridization. This study investigated a Saccharum officinarum (Green German or GG, 2n11x110) S. spontaneum (IND 81-146 or IND, 2n7x56) interspecific cross. Segregation of 193 microsatellite (SSR) loci was evaluated in the F1 progeny of 169 full-sibs of the cross. Following the two-way pseudo-testcross strategy and 'cross pollination' population type, linkage groups (LG) and phases were established for each parent map, using the criteria of LOD score 3.0 and a maximum recombination frequency of 0.35. Of the 193 markers analyzed, 61 were IND-specific, 106 were GG-specific, and 26 were heterozygous in both parents. About 78% of the markers segregated in a Mendelian fashion and 22% were distorted (as evaluated by 2-tests, P0.01). The GG map included 91 marker loci arranged into 25 LG covering 1180 cM of the officinarum genome. The IND map consisted of 46 marker loci assembled into 10 LG, which spanned 614 cM of the spontaneum genome. A specific chromosome associated with segregation distortion was detected in the female (GG) genome only, probably as a result of double reduction. The segregation patterns of the marker loci indicated a centromere-driven distortion process with the shared allelic markers (as putative centromeres) regulating the placement and association of markers with opposite phase (coupling vs repulsion) and dosage on either side. Although incomplete, the framework maps were informative with respect to segregation distortion, chromosome fusion, rearrangements, and translocations, observed in both parental genomes as a result of their merger.
Bibliography:http://dx.doi.org/10.1038/sj.hdy.6800888
http://hdl.handle.net/10113/10503
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0018-067X
1365-2540
DOI:10.1038/sj.hdy.6800888