Search Results - "Glushakova, Lyudmyla G"

Multiplexed kit based on Luminex technology and achievements in synthetic biology discriminates Zika, chikungunya, and dengue viruses in mosquitoes by Glushakova, Lyudmyla G, Alto, Barry W, Kim, Myong-Sang, Hutter, Daniel, Bradley, Andrea, Bradley, Kevin M, Burkett-Cadena, Nathan D, Benner, Steven A

“…The global expansion of dengue (DENV), chikungunya (CHIKV), and Zika viruses (ZIKV) is having a serious impact on public health. Because these arboviruses are…”
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High-throughput multiplexed xMAP Luminex array panel for detection of twenty two medically important mosquito-borne arboviruses based on innovations in synthetic biology by Glushakova, Lyudmyla G., Bradley, Andrea, Bradley, Kevin M., Alto, Barry W., Hoshika, Shuichi, Hutter, Daniel, Sharma, Nidhi, Yang, Zunyi, Kim, Myong-Jung, Benner, Steven A.

“…•The 22-fold multiplexed panel for mosquito-borne arboviruses detection is developed.•The panel differentiates viruses from genera Flavivirus, Alphavirus,…”
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Detection of chikungunya viral RNA in mosquito bodies on cationic (Q) paper based on innovations in synthetic biology by Glushakova, Lyudmyla G., Alto, Barry W., Kim, Myong Sang, Bradley, Andrea, Yaren, Ozlem, Benner, Steven A.

“…•A paper derivatized with quaternary ammonium groups was tested to collect and preserved nucleic acids from biological samples.•The simple method of samples…”
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Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA by Glushakova, Lyudmyla G., Sharma, Nidhi, Hoshika, Shuichi, Bradley, Andrea C., Bradley, Kevin M., Yang, Zunyi, Benner, Steven A.

“…Nucleic acid (NA)-targeted tests detect and quantify viral DNA and RNA (collectively xNA) to support epidemiological surveillance and, in individual patients,…”
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Optimization of cationic (Q)-paper for detection of arboviruses in infected mosquitoes by Glushakova, Lyudmyla G., Alto, Barry W., Kim, Myong-Sang, Wiggins, Keenan, Eastmond, Bradley, Moussatche, Patricia, Burkett-Cadena, Nathan D., Benner, Steven A.

“…•A cellulose-based cationic (Q) paper is a convenient platform to collect, preserve, and store nucleic acids (NA).•Factors that affected arboviral RNA…”
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Standard and AEGIS nicking molecular beacons detect amplicons from the Middle East respiratory syndrome coronavirus by Yaren, Ozlem, Glushakova, Lyudmyla G., Bradley, Kevin M., Hoshika, Shuichi, Benner, Steven A.

“…•SAMRS primers support high levels of multiplexing by not interacting with each other.•Nicking enzyme signal amplification was used to improve molecular beacon…”
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Conversion Strategy Using an Expanded Genetic Alphabet to Assay Nucleic Acids by Yang, Zunyi, Durante, Michael, Glushakova, Lyudmyla G, Sharma, Nidhi, Leal, Nicole A, Bradley, Kevin M, Chen, Fei, Benner, Steven A

“…Methods to detect DNA and RNA (collectively xNA) are easily plagued by noise, false positives, and false negatives, especially with increasing levels of…”
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Increased superoxide accumulation in pyruvate dehydrogenase complex deficient fibroblasts by Glushakova, Lyudmyla G., Judge, Sharon, Cruz, Alex, Pourang, Deena, Mathews, Clayton E., Stacpoole, Peter W.

“…The pyruvate dehydrogenase complex (PDC) oxidizes pyruvate to acetyl CoA and is critically important in maintaining normal cellular energy homeostasis…”
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AAV3-mediated transfer and expression of the pyruvate dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of human liver cancer cells by Glushakova, Lyudmyla G., Lisankie, Matthew J., Eruslanov, Evgeniy B., Ojano-Dirain, Carolyn, Zolotukhin, Irene, Liu, Chen, Srivastava, Arun, Stacpoole, Peter W.

“…Most cancers rely disproportionately on glycolysis for energy even in the presence of adequate oxygen supply, a condition known as “aerobic glycolysis”, or the…”
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An animal model of PDH deficiency using AAV8-siRNA vector-mediated knockdown of pyruvate dehydrogenase E1α by Ojano-Dirain, Carolyn, Glushakova, Lyudmyla G., Zhong, Li, Zolotukhin, Sergei, Muzyczka, Nicholas, Srivastava, Arun, Stacpoole, Peter W.

“…We evaluated the feasibility of self-complementary adeno-associated virus (scAAV) vector-mediated knockdown of the pyruvate dehydrogenase complex using small…”
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Human Blue-Opsin Promoter Preferentially Targets Reporter Gene Expression to Rat S-Cone Photoreceptors by Glushakova, Lyudmyla G, Timmers, Adrian M, Pang, Jijing, Teusner, Jacqueline T, Hauswirth, William W

“…To develop a gene therapy system that specifically targets transgene expression to S-cones of the mammalian retina, the authors coupled recombinant…”
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Does recombinant adeno-associated virus-vectored proximal region of mouse rhodopsin promoter support only rod-type specific expression in vivo? by Glushakova, Lyudmyla G, Timmers, Adrian M, Issa, Tawfik M, Cortez, Nenita G, Pang, Jijing, Teusner, Jacqueline T, Hauswirth, William W

“…We have previously found that the -385 to +86 portion of the mouse rod opsin promoter (mOP500) can limit recombinant adeno-associated virus (rAAV)-mediated…”
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495. Transfer of LEDGF to the Mouse Retina Via Systemic AAV Vector Administration by Glushakova, Lyudmyla G., Gorbatuyk, Marina, Lu, Yuanging, Song, Sihong, Zheng, Min, Shinohara, Toshimichi, Hauswirth, William W.

“…Administration of AAV vector into small rodent eyes, because of the large injection volumes relative to the size of the eye, may damage and/or evoke stress…”
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A Novel Conversion Strategy using an Expanded Genetic Alphabet to Assay Nucleic Acids by Yang, Zunyi, Durante, Michael, Glushakova, Lyudmyla G., Sharma, Nidhi, Leal, Nicole A., Bradley, Kevin M., Chen, Fei, Benner, Steven A.

“…Methods to detect DNA and RNA (collectively xNA) are easily plagued by noise, false positives, and false negatives, especially with increasing levels of…”
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Development of optimized AAV3 serotype vectors: mechanism of high-efficiency transduction of human liver cancer cells by Cheng, B, Ling, C, Dai, Y, Lu, Y, Glushakova, L G, Gee, S W Y, McGoogan, K E, Aslanidi, G V, Park, M, Stacpoole, P W, Siemann, D, Liu, C, Srivastava, A, Ling, C

“…Our recent studies have revealed that among the 10 different commonly used adeno-associated virus (AAV) serotypes, AAV3 vectors transduce human liver cancer…”
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An animal model of PDH deficiency using AAV8-siRNA vector-mediated knockdown of pyruvate dehydrogenase E1a by Ojano-Dirain, Carolyn, Glushakova, Lyudmyla G, Zhong, Li, Zolotukhin, Sergei, Muzyczka, Nicholas, Srivastava, Arun, Stacpoole, Peter W

“…We evaluated the feasibility of self-complementary adeno-associated virus (scAAV) vector-mediated knockdown of the pyruvate dehydrogenase complex using small…”
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10 Adeno-associated virus-mediated knockdown of pyruvate dehydrogenase E1 α in specific regions of the rat brain by Ojano-Dirain, Carolyn, Glushakova, Lyudmyla G., Muzyczka, Nicholas, Srivastava, Arun, Stacpoole, Peter W.

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