Search Results - "Glieder, Anton"

Regulation of Pichia pastoris promoters and its consequences for protein production by Vogl, Thomas, Glieder, Anton

“…► Biotechnologically relevant aspects of Pichia pastoris promoters are reviewed. ► The regulation of the alcohol oxidase 1 promoter (PAOX1) is discussed. ►…”
Get full text

An updated view on horseradish peroxidases: recombinant production and biotechnological applications by Krainer, Florian W., Glieder, Anton

“…Horseradish peroxidase has been the subject of scientific research for centuries. It has been used exhaustively as reporter enzyme in diagnostics and…”
Get full text

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris by Weninger, Astrid, Hatzl, Anna-Maria, Schmid, Christian, Vogl, Thomas, Glieder, Anton

“…•A CRISPR/Cas9 system has been developed for the methylotrophic yeast P. pastoris.•Several parts for expression (promoters for CAS9 and gRNAs, CAS9 CDSs…)…”
Get full text

Microbials for the production of monoclonal antibodies and antibody fragments by Spadiut, Oliver, Capone, Simona, Krainer, Florian, Glieder, Anton, Herwig, Christoph

“…Highlights • Glycosylated full length antibodies are currently produced in mammalian cells. • Antibody fragments can be produced in microbial organisms. •…”
Get full text

New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris by Vogl, Thomas, Hartner, Franz S, Glieder, Anton

“…Graphical abstract Highlights ► The application of Pichia pastoris for biopharmaceutical production is described. ► Synthetic biology approaches and…”
Get full text

Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family by Fessner, Nico D., Nelson, David R., Glieder, Anton

“…Bioprospecting for innovative basidiomycete cytochrome P450 enzymes (P450s) is highly desirable due to the fungi’s enormous enzymatic repertoire and…”
Get full text

Engineered bidirectional promoters enable rapid multi-gene co-expression optimization by Vogl, Thomas, Kickenweiz, Thomas, Pitzer, Julia, Sturmberger, Lukas, Weninger, Astrid, Biggs, Bradley W., Köhler, Eva-Maria, Baumschlager, Armin, Fischer, Jasmin Elgin, Hyden, Patrick, Wagner, Marlies, Baumann, Martina, Borth, Nicole, Geier, Martina, Ajikumar, Parayil Kumaran, Glieder, Anton

“…Numerous synthetic biology endeavors require well-tuned co-expression of functional components for success. Classically, monodirectional promoters (MDPs) have…”
Get full text

Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology by Näätsaari, Laura, Mistlberger, Beate, Ruth, Claudia, Hajek, Tanja, Hartner, Franz S, Glieder, Anton

“…Targeted gene replacement to generate knock-outs and knock-ins is a commonly used method to study the function of unknown genes. In the methylotrophic yeast…”
Get full text

Expression of lignocellulolytic enzymes in Pichia pastoris by Mellitzer, Andrea, Weis, Roland, Glieder, Anton, Flicker, Karlheinz

“…Sustainable utilization of plant biomass as renewable source for fuels and chemical building blocks requires a complex mixture of diverse enzymes, including…”
Get full text

Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection by Weiss, Florian, Requena-Moreno, Guillermo, Pichler, Carsten, Valero, Francisco, Glieder, Anton, Garcia-Ortega, Xavier

“…Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes. The clonal…”
Get full text

Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors by Vogl, Thomas, Sturmberger, Lukas, Fauland, Pia C., Hyden, Patrick, Fischer, Jasmin E., Schmid, Christian, Thallinger, Gerhard G., Geier, Martina, Glieder, Anton

“…Carbon source regulated promoters are well‐studied standard tools for controlling gene expression. Acquiring control over the natural regulation of promoters…”
Get full text

Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris by Vogl, Thomas, Fischer, Jasmin Elgin, Hyden, Patrick, Wasmayer, Richard, Sturmberger, Lukas, Glieder, Anton

“…Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris , Pp ), Hansenula polymorpha ( Hp ), Candida boidinii ( Cb ) and Pichia methanolica (…”
Get full text

Yeast cell factories for fine chemical and API production by Pscheidt, Beate, Glieder, Anton

“…This review gives an overview of different yeast strains and enzyme classes involved in yeast whole-cell biotransformations. A focus was put on the synthesis…”
Get full text

Synthetic Core Promoters for Pichia pastoris by Vogl, Thomas, Ruth, Claudia, Pitzer, Julia, Kickenweiz, Thomas, Glieder, Anton

“…Synthetic promoters are commonly used tools for circuit design or high level protein production. Promoter engineering efforts in yeasts, such as Saccharomyces…”
Get full text

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway by Krainer, Florian W, Dietzsch, Christian, Hajek, Tanja, Herwig, Christoph, Spadiut, Oliver, Glieder, Anton

“…ΒACKGROUND: The methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production and is able to use methanol as a…”
Get full text

Regulation of methanol utilisation pathway genes in yeasts by Hartner, Franz S, Glieder, Anton

“…Methylotrophic yeasts such as Candida boidinii, Hansenula polymorpha, Pichia methanolica and Pichia pastoris are an emerging group of eukaryotic hosts for…”
Get full text

Publisher Correction: Engineered bidirectional promoters enable rapid multi-gene co-expression optimization by Vogl, Thomas, Kickenweiz, Thomas, Pitzer, Julia, Sturmberger, Lukas, Weninger, Astrid, Biggs, Bradley W., Köhler, Eva-Maria, Baumschlager, Armin, Fischer, Jasmin Elgin, Hyden, Patrick, Wagner, Marlies, Baumann, Martina, Borth, Nicole, Geier, Martina, Ajikumar, Parayil Kumaran, Glieder, Anton

“…A Correction to this paper has been published: https://doi.org/10.1038/s41467-021-21369-z…”
Get full text

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris by Vogl, Thomas, Sturmberger, Lukas, Kickenweiz, Thomas, Wasmayer, Richard, Schmid, Christian, Hatzl, Anna-Maria, Gerstmann, Michaela A, Pitzer, Julia, Wagner, Marlies, Thallinger, Gerhard G, Geier, Martina, Glieder, Anton

“…The heterologous expression of biosynthetic pathways for pharmaceutical or fine chemical production requires suitable expression hosts and vectors. In…”
Get full text

High-quality genome sequence of Pichia pastoris CBS7435 by Küberl, Andreas, Schneider, Jessica, Thallinger, Gerhard G., Anderl, Ingund, Wibberg, Daniel, Hajek, Tanja, Jaenicke, Sebastian, Brinkrolf, Karina, Goesmann, Alexander, Szczepanowski, Rafael, Pühler, Alfred, Schwab, Helmut, Glieder, Anton, Pichler, Harald

“…The methylotrophic yeast Pichia pastoris ( Komagataella phaffii) CBS7435 is the parental strain of commonly used P. pastoris recombinant protein production…”
Get full text

Bi-directionalized promoter systems allow methanol-free production of hard-to-express peroxygenases with Komagataella Phaffii by Besleaga, Mihail, Zimmermann, Christian, Ebner, Katharina, Mach, Robert L, Mach-Aigner, Astrid R, Geier, Martina, Glieder, Anton, Spadiut, Oliver, Kopp, Julian

“…Heme-incorporating peroxygenases are responsible for electron transport in a multitude of organisms. Yet their application in biocatalysis is hindered due to…”
Get full text