Some peculiarities of pulmonary clearance mechanisms in rats after intratracheal instillation of magnetite (Fe3O4) suspensions with different particle sizes in the nanometer and micrometer ranges: are we defenseless against nanoparticles?

We studied differences between phagocytic responses to nanoparticles (NPs) versus microparticles in the pulmonary region by synthesizing magnetite of different sizes and instilling suspensions of these particles intratracheally into rats' lungs. Ten and 50 nm particles caused a greater increase...

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Bibliographic Details
Published in:	International journal of occupational and environmental health Vol. 16; no. 4; p. 508
Main Authors:	Katsnelson, Boris, Privalova, Larissa I, Kuzmin, Sergey V, Degtyareva, Tamara D, Sutunkova, Marina P, Yeremenko, Olga S, Minigalieva, Ilzira A, Kireyeva, Ekaterina P, Khodos, Mark Y, Kozitsina, Alisa N, Malakhova, Natalya A, Glazyrina, Julia A, Shur, Vladimir Y, Shishkin, Eugene I, Nikolaeva, Ekaterina V
Format:	Journal Article
Language:	English
Published:	England 01-10-2010
Subjects:	Animals Bronchoalveolar Lavage Fluid - cytology Female Ferric Compounds - administration & dosage Ferric Compounds - adverse effects Lung - drug effects Lung - immunology Lung - pathology Macrophages, Alveolar - drug effects Macrophages, Alveolar - immunology Magnetite Nanoparticles - administration & dosage Magnetite Nanoparticles - adverse effects Neutrophils - drug effects Neutrophils - immunology Particle Size Phagocytosis Rats
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Summary:	We studied differences between phagocytic responses to nanoparticles (NPs) versus microparticles in the pulmonary region by synthesizing magnetite of different sizes and instilling suspensions of these particles intratracheally into rats' lungs. Ten and 50 nm particles caused a greater increase in cell counts of the bronchoalveolar lavage fluid (BALF) than the instillation of microparticles. The response to 10 nm particles was weaker than to 50 nm ones, and the smaller NPs were more cytotoxic; both were more cytotoxic than the microparticles. Phagocytic activity was also studied using optical and atomic force microscopy. Phagocytes were more "loaded" in the lungs instilled with 10 nm particles as compared with those instilled with 50 nm particles; NPs of both sizes were engulfed more avidly than microparticles. We found in a separate comparative experiment that magnetite NPs were more cytotoxic than titanium dioxide and quartz suspensions having particle size distribution typical of industrial dusts.
ISSN:	1077-3525
DOI:	10.1179/oeh.2010.16.4.508