Generation of induced pluripotent stem cell line RCPCMi004-A derived from patient with Parkinson's disease with deletion of the exon 2 in PARK2 gene

IPSC line RCPCMi004-A was generated from skin fibroblasts collected from a male patient with early onset Parkinson's disease. The patient carries a heterozygous deletion of the exon 2 of PARK2 gene. The reprogramming of fibroblasts was performed with Sendai viruses containing Oct-4, Sox-2, Klf-...

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Bibliographic Details
Published in:	Stem cell research Vol. 44; p. 101733
Main Authors:	Shuvalova, L.D., Eremeev, A.V., Bogomazova, A.N., Novosadova, E.V., Zerkalenkova, E.A., Olshanskaya, Yu.V., Fedotova, E.Y., Glagoleva, E.S., Illarioshkin, S.N., Lebedeva, O.S., Lagarkova, M.A.
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 01-04-2020 Elsevier
Online Access:	Get full text
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Summary:	IPSC line RCPCMi004-A was generated from skin fibroblasts collected from a male patient with early onset Parkinson's disease. The patient carries a heterozygous deletion of the exon 2 of PARK2 gene. The reprogramming of fibroblasts was performed with Sendai viruses containing Oct-4, Sox-2, Klf-4 and c-Myc. Pluripotency was confirmed by immunofluorescence, RT-PCR, and formation of embryoid bodies. The RCPCMi004-A cell line carries the same deletion in PARK2 gene. The RCPCMi004-A cell line can be used to model Parkinson's disease in vitro.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1873-5061 1876-7753
DOI:	10.1016/j.scr.2020.101733