Lipopolysaccharide toxin can directly stimulate the intracellular accumulation of lipids and their secretion into medium in the primary culture of rabbit hepatocytes

Low doses (0.1–0.3 μg ml per 10 6 cells) of the lipopolysaccharide toxin (LPS) from Salmonella typhimurium were shown to increase (after an 18 h incubation) the intracellular content of free cholesterol (CH), esterified cholesterol (EC) and triglycerides (TG) by 30–40% in the primary culture of rabb...

Full description

Saved in:

Bibliographic Details
Published in:	FEBS letters Vol. 256; no. 1; pp. 155 - 158
Main Authors:	Victorov, A.V., Gladkaya, E.M., Novikov, D.K., Kosykh, V.A., Yurkiv, V.A.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 09-10-1989 Elsevier
Subjects:	Animal cells Animals Bacterial Toxins - pharmacology Bacteriology Biological and medical sciences Cell cultures. Hybridization. Fusion Cells, Cultured CH, free cholesterol Cholesterol - metabolism Cholesterol Esters - metabolism Culture Media - analysis Dose-Response Relationship, Drug EC, esterified cholesterol Fundamental and applied biological sciences. Psychology HPLC, high-performance liquid chromatography HPTLC, high-performance thin-layer chromatography Intracellular Membranes - metabolism Lipid Metabolism Lipopolysaccharides - pharmacology Liver - drug effects Liver - metabolism LPS, lipopolysaccharide toxin Microbiology Molecular and cellular biology NL, neutrallipids Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Phospholipids - analysis PL, phospholipids Rabbits Salmonella typhimurium TG,triglycerides TNF, tumor necrosis factor Triglycerides - metabolism HPLC, high-performance liquid chromatography LPS, lipopolysaccharide toxin CH, free cholesterol TNF, tumor necrosis factor HPTLC, high-performance thin-layer chromatography TG,triglycerides NL, neutrallipids EC, esterified cholesterol PL, phospholipids Induced hyperlipemia Bacteria Secretion Salmonella typhimurium Enterobacteriaceae Cell culture High performance Radiolabelling Rabbit Lipids Stimulation Lagomorpha Thin layer chromatography Toxin Vertebrata Mammalia Hepatocyte Lipopolysaccharide Hyperlipemia Intracellular Escherichieae Liquid scintillation
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Low doses (0.1–0.3 μg ml per 10 6 cells) of the lipopolysaccharide toxin (LPS) from Salmonella typhimurium were shown to increase (after an 18 h incubation) the intracellular content of free cholesterol (CH), esterified cholesterol (EC) and triglycerides (TG) by 30–40% in the primary culture of rabbit hepatocytes. A similar increase was found for the incorporation of [ 14C]acetate into these lipids. The concentration of lipids in cultural medium, under these conditions, was also augmented: by 30–40% for CH; by 50–60% for TG and by 60–80% for EC. Higher doses (up to 50 μg ml ) of LPS hardly affected the lipid content in hepatocytes but strongly (by two-fold) inhibited the secretion of lipids. It is suggested that in vivo low concentrations of LPS in bloodstream (in the absence of conspicuous pathology) might induce hyperlipidemia directly influencing on hepatic cells, while, under the higher concentrations of LPS, hyperlipidemia caused by cachectin (or tumor necrosis factor) is probably observed. Lipopolysaccharide toxin; Hyperlipidemia; (Rabbit hepatocyte)
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(89)81738-7