Search Results - "Gladilin, A K"

Enzyme-polyelectrolyte complexes in water-ethanol mixtures: Negatively charged groups artificially introduced into α-chymotrypsin provide additional activation and stabilization effects by Kudryashova, Elena V., Gladilin, Alexander K., Vakurov, Alexander V., Heitz, Frederic, Levashov, Andrey V., Mozhaev, Vadim V.

“…Formation of noncovalent complexes between α‐chymotrypsin (CT) and a polyelectrolyte, polybrene (PB), has been shown to produce two major effects on enzymatic…”
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Enzyme stability in systems with organic solvents by Gladilin, A K, Levashov, A V

“…This review deals with enzyme stability in organic solvent systems. Based on the current state of the problem, catalytic activity is chosen as the main tool…”
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Activity and stability of native and modified subtilisins in various media by Bacheva, A V, Baibak, O V, Belyaeva, A V, Oksenoit, E S, Velichko, T I, Lysogorskaya, E N, Gladilin, A K, Lozinsky, V I, Filippova, I Yu

“…The activity and stability of native subtilisin 72, its complex with poly(acrylic acid), and subtilisin covalently attached to poly(vinyl alcohol) cryogel were…”
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Kinetic behavior of phosphotriesterase and its non-covalent complexes with polyelectrolyte in systems with polar and non-polar organic solvents by SERGEEVA, V. S, EFREMENKO, E. N, KAZANKOV, G. M, GLADILIN, A. K, VARFOLOMEEV, S. D

“…Soluble phosphotriesterase from E. coli DH5 alpha together with E. coli DH5 alpha cells with the phosphotriesterase activity were co-immobilized into…”
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α-Chymotrypsin immobilized on ferromagnetic particles coated with titanium oxide: Production and catalytic characterization by Izmailov, A. F., Kiselev, M. V., Vakurov, A. V., Gladilin, A. K., Levashov, A. V.

“…Immobilization of α-chymotrypsin on magnetic particles with stable coat with titanium oxides as a main constituent allowed the biocatalytic system to be…”
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Improvement of Electrochemical Biosensors Using Enzyme Immobilization from Water−Organic Mixtures with a High Content of Organic Solvent by Karyakin, Arkady A, Karyakina, Elena E, Gorton, Lo, Bobrova, Oksana A, Lukachova, Lylia V, Gladilin, Alexander K, Levashov, Andrey V

“…We propose immobilizing enzymes into Nafion membranes by suspending the enzyme in a water−ethanol mixture with a high (>90%) ethanol content, followed by…”
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Reversed micelles of polymeric surfactants on nonpolar organic solvents : a new microheterogeneous medium for enzymatic reactions by KHMELNITSKY, Y. L, GLADILIN, A. K, ROUBAILO, V. L, MARTINEK, K, LEVASHOV, A. V

“…A new microheterogeneous non-aqueous medium for enzymatic reactions, based on reversed micelles of a polymeric surfactant, was suggested. The surfactant termed…”
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Determination of Cyclosporin A in 20% Ethanol by a Magnetic Beads-Based Immunofluorescence Assay by Kiselev, Maxim V., Gladilin, Alexander K., Melik-Nubarov, Nikolay S., Sveshnikov, Peter G., Miethe, Peter, Levashov, Andrey V.

“…A rapid magnetic beads-based immunoassay for the immunodepressant drug cyclosporin A (CsA) has been developed. The method allows CsA determination in medium…”
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International chemistry olympiad and its role in chemical education by Eremin, V. V., Gladilin, A. K.

“…International Chemistry Olympiad (IChQ) is the annual world competition for high school students — the winner of national olympiads. The level of this…”
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Dry enzyme-polymer complexes: Stable organosoluble biocatalysts for nonaqueous enzymology by Vakurov, A V, Gladilin, A K, Levashov, A V, Khmelnitsky, Y L

“…Complexes of chymotrypsin and laccase with amphiphilic alkylated poly(ethyleneimine) (CEPEI) were obtained in a dry state by evaporation of nonaqueous…”
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Formation of quasi-regular compact structure of poly(methacrylic acid) upon an interaction with α-chymotrypsin by Kudryashova, Elena V., Gladilin, Alexander K., Izumrudov, Vladimir A., van Hoek, Arie, Visser, Antonie J.W.G., Levashov, Andrey V.

“…Structure and dynamic properties of free poly(methacrylic acid) (PMA) and PMA complexed with α-chymotrypsin (CT) were studied using the time resolved…”
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Enzyme-polyelectrolyte noncovalent complexes as catalysts for reactions in binary mixtures of polar organic solvents with water by Gladilin, AlexanderK, Kudryashova, ElenaV, Vakurov, AlexanderV, Izumrudov, VladimirA, Mozhaev, VadimV, Levashov, AndreyV

“…The formation of non-covalent complexes with polyelectrolytes has been suggested to enhance the resistance of enzymes towards inactivation by organic solvents…”
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Polycation stabilization of the enzyme LysK lysing Staphylococcus Aureus cells by Filatova, L. Yu, Becker, S. C., Donovan, D. M., Gladilin, A. K., Klyachko, N. L.

“…The influence of polycation (polybrene) on the activity and stability of LysK enzyme lysing Staphylococcus Aureus cells was studied and a tenfold increase in…”
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Non-covalent enzyme-polyelectrolyte complexes as self-buffered catalysts for use in low-water organic media by Vakurov, Alexander V., Gladilin, Alexander K., Partridge, Johann, Mozhaev, Vadim V., Levashov, Andrey V., Halling, Peter J.

“…When free chymotrypsin is used to catalyse hydrolysis of N-acetyl tyrosine ethyl ester in 90% acetonitrile, the reaction rate soon falls because of the…”
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[alpha]-Chymotrypsin immobilized on ferromagnetic particles coated with titanium oxide: Production and catalytic characterization by Izmailov, A F, Kiselev, M V, Vakurov, A V, Gladilin, A K, Levashov, A V

“…Immobilization of α-chymotrypsin on magnetic particles with stable coat with titanium oxides as a main constituent allowed the biocatalytic system to be…”
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Alpha-chymotrypsin immobilized on ferromagnetic particles coated with titanium oxide: production and catalytic properties by Izmaĭlov, A F, Kiselev, M V, Vakurov, A V, Gladilin, A K, Levashov, A V

“…Immobilization of alpha-chymotrypsin on magnetic particles with stable coat with titanium oxides as a main constituent allowed the biocatalytic system to be…”
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Stabilization and activation of α-chymotrypsin in water–organic solvent systems by complex formation with oligoamines by Kudryashova, Elena V., Artemova, Tatiana M., Vinogradov, Alexei A., Gladilin, Alexander K., Mozhaev, Vadim V., Levashov, Andrey V.

“…Formation of enzyme–oligoamine complexes was suggested as an approach to obtain biocatalysts with enhanced resistance towards inactivation in water–organic…”
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Dry enzyme-polymer complexes : stable organosoluble biocatalysts for nonaqueous enzymology by VAKUROV, A. V, GLADILIN, A. K, LEVASHOV, A. V, KHMLNITSKY, YU. L

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Enzyme-polyelectrolyte noncovalent complexes as catalysts for reactions in binary mixtures of polar organic solvents with water by GLADILIN, A. K, KUDRYASHOVA, E. V, VAKUROV, A. V, IZUMRUDOV, V. A, MOZHAEV, V. V, LEVASHOV, A. V

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Detergentless microemulsions as media for enzymatic reactions: catalytic properties of laccase in the ternary system hexane-2-propanol-water by KHMELNITSKY, Y. L, GLADILIN, A. K, NEVEROVA, I. N, LEVASHOV, A. V, MARTINEK, K

“…The catalytic activity and stability of laccase in the detergentless ternary system hexane - 2-propanol - water were studied. In the microemulsion region of…”
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