Search Results - "Girhard, Marco"

Cytochrome P450 Monooxygenases in Biotechnology and Synthetic Biology by Urlacher, Vlada B., Girhard, Marco

“…Cytochromes P450 (P450 or CYP) are heme-containing enzymes that catalyze the introduction of one atom of molecular oxygen into nonactivated C–H bonds, often in…”
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Cytochrome P450 monooxygenases: an update on perspectives for synthetic application by Urlacher, Vlada B, Girhard, Marco

“…Cytochrome P450 monooxygenases (P450s) are versatile biocatalysts that catalyze the regio- and stereospecific oxidation of non-activated hydrocarbons under mild…”
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Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes by Decembrino, Davide, Girhard, Marco, Urlacher, Vlada B.

“…Laccases are multi‐copper oxidases that catalyze the oxidation of various electron‐rich substrates with concomitant reduction of molecular oxygen to water. The…”
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Synthesis of (-)-deoxypodophyllotoxin and (-)-epipodophyllotoxin via a multi-enzyme cascade in E. coli by Decembrino, Davide, Raffaele, Alessandra, Knöfel, Ronja, Girhard, Marco, Urlacher, Vlada B

“…The aryltetralin lignan (-)-podophyllotoxin is a potent antiviral and anti-neoplastic compound that is mainly found in Podophyllum plant species. Over the…”
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Spotlight on CYP4B1 by Röder, Annika, Hüsken, Saskia, Hutter, Michael C, Rettie, Allan E, Hanenberg, Helmut, Wiek, Constanze, Girhard, Marco

“…The mammalian cytochrome P450 monooxygenase CYP4B1 can bioactivate a wide range of xenobiotics, such as its defining/hallmark substrate 4-ipomeanol leading to…”
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Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system by Girhard, Marco, Machida, Kazuhiro, Itoh, Masashi, Schmid, Rolf D, Arisawa, Akira, Urlacher, Vlada B

“…(+)-Nootkatone (4) is a high added-value compound found in grapefruit juice. Allylic oxidation of the sesquiterpene (+)-valencene (1) provides an attractive…”
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Heterologous Lignan Production in Stirred-Tank Reactors—Metabolomics-Assisted Bioprocess Development for an In Vivo Enzyme Cascade by Andrea Steinmann, Maurice Finger, Christian Nowacki, Davide Decembrino, Georg Hubmann, Marco Girhard, Vlada B. Urlacher, Stephan Lütz

“…Towards establishing a prospective industrial microbial lignan production process, we set up and investigated the biotransformation of coniferyl alcohol to…”
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Biocatalysis: Key to Selective Oxidations by Schulz, Sebastian, Girhard, Marco, Urlacher, Vlada B.

“…Selective oxidations of readily available organic molecules can lead directly to high‐value chiral compounds. Although modern chemical catalysts have…”
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A targeted metabolomics method for extra- and intracellular metabolite quantification covering the complete monolignol and lignan synthesis pathway by Steinmann, Andrea, Schullehner, Katrin, Kohl, Anna, Dickmeis, Christina, Finger, Maurice, Hubmann, Georg, Jach, Guido, Commandeur, Ulrich, Girhard, Marco, Urlacher, Vlada B., Lütz, Stephan

“…Microbial synthesis of monolignols and lignans from simple substrates is a promising alternative to plant extraction. Bottlenecks and byproduct formation…”
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Regioselective hydroxylation of norisoprenoids by CYP109D1 from Sorangium cellulosum So ce56 by Khatri, Yogan, Girhard, Marco, Romankiewicz, Anna, Ringle, Michael, Hannemann, Frank, Urlacher, Vlada B, Hutter, Michael C, Bernhardt, Rita

“…Sesquiterpenes are particularly interesting as flavorings and fragrances or as pharmaceuticals. Regio- or stereoselective functionalizations of terpenes are…”
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P450BM3-Catalyzed Oxidations Employing Dual Functional Small Molecules by Sébastien J.-P. Willot, Florian Tieves, Marco Girhard, Vlada B. Urlacher, Frank Hollmann, Gonzalo de Gonzalo

“…A set of dual functional small molecules (DFSMs) containing different amino acids has been synthesized and employed together with three different variants of…”
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Fusion to Hydrophobin HFBI Improves the Catalytic Performance of a Cytochrome P450 System by Schulz, Sebastian, Schumacher, Dominik, Raszkowski, Daniel, Girhard, Marco, Urlacher, Vlada B

“…Cytochrome P450 monooxygenases (P450) are heme-containing enzymes that oxidize a broad range of substrates in the presence of molecular oxygen and NAD(P)H. For…”
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Draft Genome Sequences of Three Actinobacteria Strains Presenting New Candidate Organisms with High Potentials for Specific P450 Cytochromes by Grumaz, Christian, Vainshtein, Yevhen, Kirstahler, Philipp, Luetz, Stephan, Kittelmann, Matthias, Schroer, Kirsten, Eggimann, Fabian K, Czaja, Rico, Vogel, Andreas, Hilberath, Thomas, Worsch, Anne, Girhard, Marco, Urlacher, Vlada B, Sandberg, Marcel, Sohn, Kai

“…The three strains DSM 40041 DSM 535, and DSM 40063 were described to selectively oxyfunctionalize several drugs. Here, we present their draft genomes to…”
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Multienzymatische Kaskaden zur Synthese von Feinchemikalien und Polymervorstufen by Girhard, Marco, Ricklefs, Esther, Urlacher, Vlada B.

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Three-steps in one-pot: whole-cell biocatalytic synthesis of enantiopure (+)- and (-)-pinoresinol via kinetic resolution by Ricklefs, Esther, Girhard, Marco, Urlacher, Vlada B

“…Pinoresinol is a high-value plant-derived lignan with multiple health supporting effects. Enantiomerically pure pinoresinol can be isolated from natural…”
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In situ formation of H2O2 for P450 peroxygenases by Paul, Caroline E., Churakova, Ekaterina, Maurits, Elmer, Girhard, Marco, Urlacher, Vlada B., Hollmann, Frank

“…[Display omitted] An in situ H2O2 generation approach to promote P450 peroxygenases catalysis was developed through the use of the nicotinamide cofactor…”
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Characterization of the versatile monooxygenase CYP109B1 from Bacillus subtilis by Girhard, Marco, Klaus, Tobias, Khatri, Yogan, Bernhardt, Rita, Urlacher, Vlada B

“…The oxidizing activity of CYP109B1 from Bacillus subtilis was reconstituted in vitro with various artificial redox proteins including putidaredoxin reductase…”
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Synthesis of -epipodophyllotoxin via a multi-enzyme cascade in E. coli by Decembrino, Davide, Raffaele, Alessandra, Knöfel, Ronja, Girhard, Marco, Urlacher, Vlada B

“…The aryltetralin lignan (-)-podophyllotoxin is a potent antiviral and anti-neoplastic compound that is mainly found in Podophyllum plant species. Over the…”
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Novel insights into oxidation of fatty acids and fatty alcohols by cytochrome P450 monooxygenase CYP4B1 by Thesseling, Florian A., Hutter, Michael C., Wiek, Constanze, Kowalski, John P., Rettie, Allan E., Girhard, Marco

“…CYP4B1 is an enigmatic mammalian cytochrome P450 monooxygenase acting at the interface between xenobiotic and endobiotic metabolism. A prominent CYP4B1…”
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Assembly of Plant Enzymes in E. coli for the Production of the Valuable (−)-Podophyllotoxin Precursor (−)-Pluviatolide by Decembrino, Davide, Ricklefs, Esther, Wohlgemuth, Stefan, Girhard, Marco, Schullehner, Katrin, Jach, Guido, Urlacher, Vlada B

“…Lignans are plant secondary metabolites with a wide range of reported health-promoting bioactivities. Traditional routes toward these natural products involve,…”
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