Search Results - "Giovanni‐De‐Simone, S"

Purification and partial characterization of an extracellular serine‐proteinase of Streptomyces cyaneus isolated from Brazilian cerrado soil by Petinate, S.D.G, Branquinha, M.H, Coelho, R.R.R, And, A.B. Vermelho, Giovanni‐De‐Simone, S

“…Streptomyces cyaneus, a micro‐organism isolated from Brazilian cerrado soil, produces an extracellular proteinase (SCP), which was purified 22‐fold to…”
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A novel butyrylcholinesterase from serum of Leporinus macrocephalus, a Neotropical fish by Salles, J.B., Cunha Bastos, V.L.F., Silva Filho, M.V., Machado, O.L.T., Salles, C.M.C., Giovanni de Simone, S., Cunha Bastos, J.

“…We show here that serum of piaussu, a Neotropical characin fish, has the highest butyrylcholinesterase activity so far described for humans and fish. To…”
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Purification and partial characterization of Trypanosoma cruzi triosephosphate isomerase by Bourguignon, S C, Meirelles, M N, Pacheco, R S, De Simone, S G

“…The enzyme triosephosphate isomerase (TPI, EC 5.3.1.1) was purified from extracts of epimastigote forms of Trypanosoma cruzi. The purification steps included:…”
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Effects of serine protease inhibitors on viability and morphology of Leishmania (Leishmania) amazonensis promastigotes by SILVA-LOPEZ, R. E, MORGADO-DIAZ, J. A, CHAVEZ, M. A, GIOVANNI-DE-SIMONE, S

“…To investigate the importance of serine proteases in Leishmania amazonensis promastigotes, we analyzed the effects of classical serine protease inhibitors and…”
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Molecular modeling approaches for determining gene function: application to a putative poly-A binding protein from Leishmania amazonensis (LaPABP) by Silva Junior, F P, Veyl, F Z, Clos, J, De Simone, S Giovanni

“…The great expansion in the number of genome sequencing projects has revealed the importance of computational methods to speed up the characterization of…”
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Identification and characterization of proteases from skin mucus of tambacu, a Neotropical hybrid fish by Salles, C. M. C, Gagliano, P, Leitão, S. A. T, Salles, J. B, Guedes, H. L. M, Cassano, V. P. F, De-Simone, S. Giovanni

“…Skin secretions of fishes constitute a rich source of proteins with a broad spectrum of antimicrobial properties. We report here the characterization of…”
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Single-step purification of crotapotin and crotactine from Crotalus durissus terrificus venom using preparative isoelectric focusing by Aguiar, A S, Melgarejo, A R, Alves, C R, Giovanni-De-Simone, S

“…We describe the isolation of crotoxin, a presynaptic B-neurotoxin, as well as its subunits B (crotactine) and A (crotapotin) from lyophilized Crotalus durissus…”
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Subcellular localization of an extracellular serine protease in Leishmania (Leishmania) amazonensis by SILVA-LOPEZ, R. E, MORGADO-DIAZ, J. A, ALVES, C. R, CORTE-REAL, S, GIOVANNI-DE-SIMONE, S

“…Extracellular proteolytic activity was detected in a Leishmania ( L.) amazonensis culture supernatant and a 56-kDa protein was purified using (NH4)2SO4…”
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Exploring the subsite specificity of Schistosoma mansoni aspartyl hemoglobinase through comparative molecular modelling by Silva, F.P., Ribeiro, F., Katz, N., Giovanni-De-Simone, S.

“…Blood flukes of the genus Schistosoma currently infect millions of people in tropical and subtropical countries. An enzyme playing a major role in hemoglobin…”
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Purification and partial characterization of a thrombin-like/gyroxin enzyme from bushmaster ( Lachesis muta rhombeata) venom by Aguiar, A.S., Alves, C.R., Melgarejo, A., Giovanni-De-Simone, S.

“…The acidic coagulating enzyme of the L. m. rhombeata venom was purified to homogeneity using one step on preparative isoelectric focusing followed by gel…”
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Isolation and immunological analysis of Trypanosoma cruzi glycolipids by Giovanni De Simone, S, Pinho, R T, Vanni, C M, Pontes de Carvalho, L C

“…A water-soluble glycolipidic fraction from Trypanosoma cruzi was isolated using a mixture of hexane and isopropanol. Analysis by SDS-polyacrylamide gel…”
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Purification and partial characterization of creatine kinase from electric organ of Electrophorus electricus (L.) by Batista e Silva, C.M., Tavares, N.Nunes, Giovanni-De-Simone, S., Nery da Matta, A., Hassón-Voloch, A.

“…The present investigation deals with the purification and the partial characterization of the soluble creatine kinase (CK) isoenzyme, isolated from the…”
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Detrimental effect of nitric oxide on Trypanosoma cruzi and Leishmania major like cells by Bourguignon, S.C, Alves, C.R, Giovanni-De-Simone, S

“…The production of nitric oxide (NO) by activated macrophages has been reported to be a non-specific immune-effect mechanism against several parasites. In this…”
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Purification and Partial Characterization of Glyceraldehyde-Phosphate Dehydrogenase from Electric Organ of Electrophorus electricus (L.) by Giovanni-De-Simone, S., Hassón-Voloch, A., Batista-e-Silva, C., Nery-da-Matta, A.

“…The glyceraldehyde-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) was purified to homogeneity from electric organ of Electrophorus electricus (L.) by a…”
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Heterogeneity of cysteine proteinases in Leishmania braziliensis and Leishmania major by Alves, C R, Marzochi, M C, Giovanni-de-Simone, S

“…Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with acrylamide copolymerized with gelatin (substrate-SDS-PAGE) was used to compare…”
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BJ-48, a novel thrombin-like enzyme from the Bothrops jararacussu venom with high selectivity for Arg over Lys in P1: Role of N-glycosylation in thermostability and active site accessibility by Silva-Junior, Floriano P., Guedes, Herbert L.M., Garvey, Laura C., Aguiar, Aniesse S., Bourguignon, Saulo C., Di Cera, Enrico, Giovanni-De-Simone, Salvatore

“…BJ-48, a serine protease from the venom of Bothrops jararacussu, was purified to homogeneity using affinity chromatography on p-aminobenzamidine-agarose…”
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Rapid method using high performance liquid chromatography for the purification of tetanus toxoid by Silveira, I A, Jessouroun, E, de Simone, S G

“…The tetanus toxin is produced by the Clostridium tetani and appears into the medium after lysis of the bacteria (B Bizzini et al. 1969 Ann Inst Pasteur 116:…”
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Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains by Guaycurus, T V, Vicente, A C, de Simone, S G, Rabinovitch, L

“…Extracellular proteins produced by Bacillus cereus AL-42 and AL-15 were fractioned by chromatography on QAE-Sephadex and Sephadex G75. This last…”
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A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide by Pinho, R T, Dutra, H S, Giovanni-De-Simone, S, de Carvalho, L C

“…Glass wool, hydrophilic cotton wool, non-electrically charged BIO-GEL P2 and common tissue paper columns were used to purify trypomastigotes from a mixed…”
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Leishmania (Leishmania) amazonensis: purification and characterization of a promastigote serine protease by da Silva-Lopez, Raquel Elisa, Giovanni-De-Simone, Salvatore

“…Pathogenic protozoan proteases play crucial roles in the host–parasite interaction, and its characterization contributes to the understanding of protozoan…”
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