Genomic characterization of Brazilian hepatitis C virus genotypes 1a and 1b

Genomic characterization of Brazilian hepatitis C virus genotypes 1a and 1b

Parts of 5' non-coding (5' NC) and of E1 envelope regions of the hepatitis C virus (HCV) genome were amplified from sera of 26 Brazilian anti-HCV antibody-positive patients using the reverse transcription-polymerase chain reaction (RT-PCR). Fourteen samples were PCR positive with primers f...

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Bibliographic Details
Published in:Brazilian journal of medical and biological research Vol. 30; no. 3; pp. 339 - 345
Main Authors: Ginabreda, M G, Yoshida, C F, Niel, C
Format: Journal Article
Language:English
Published: Brazil Associação Brasileira de Divulgação Científica 01-03-1997
Subjects:
Adult
Base Sequence
BIOLOGY
Brazil
Brazilian isolates
DNA, Viral - analysis
Female
Genome, Viral
Genotype
genotype 1a
genotype 1b
Hepacivirus - classification
Hepacivirus - genetics
Hepatitis C virus
Humans
Male
MEDICINE, RESEARCH & EXPERIMENTAL
Molecular Sequence Data
nucleotide sequence
Brazil
nucleotide sequence
hepatitis C virus
genotype 1a
genotype 1b
Brazilian isolates
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Summary:Parts of 5' non-coding (5' NC) and of E1 envelope regions of the hepatitis C virus (HCV) genome were amplified from sera of 26 Brazilian anti-HCV antibody-positive patients using the reverse transcription-polymerase chain reaction (RT-PCR). Fourteen samples were PCR positive with primers from the 5' NC region and 8 of them were also positive with primers from the E1 region. A genomic segment of 176 bp from the E1 region of 7 isolates was directly sequenced from PCR products. The sequences were compared with those of HCV strains isolated in other countries and the Brazilian isolates were classified by phylogenetic analysis into genotypes 1a and 1b. This could have a clinical importance since it has been shown that individuals infected with type 1 viruses are less likely to respond to treatment with interferon than individuals infected with types 2 and 3 viruses. Two quasispecies isolated from the same patient with an interval of 13 months differed by two base substitutions (1.1%). The sequence of another isolate presented a three-nucleotide deletion at codon 329.
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ISSN:0100-879X
1414-431X
0100-879X
1414-431X
DOI:10.1590/S0100-879X1997000300006