Search Results - "Gimble, Frederick S."

To nick or not to nick: comparison of I-SceI single- and double-strand break-induced recombination in yeast and human cells by Katz, Samantha S, Gimble, Frederick S, Storici, Francesca

“…Genetic modification of a chromosomal locus to replace an existing dysfunctional allele with a corrected sequence can be accomplished through targeted gene…”
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NgAgo possesses guided DNA nicking activity by Lee, Kok Zhi, Mechikoff, Michael A, Kikla, Archana, Liu, Arren, Pandolfi, Paula, Fitzgerald, Kevin, Gimble, Frederick S, Solomon, Kevin V

“…Abstract Prokaryotic Argonautes (pAgos) have been proposed as more flexible tools for gene-editing as they do not require sequence motifs adjacent to their…”
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Structure-Function Studies of Two Yeast Homing Endonucleases that Evolved to Cleave Identical Targets with Dissimilar Rates and Specificities by Nawimanage, Rasika R., Yuan, Ziyan, Casares, Mackenzie, Joshi, Rakesh, Lohman, Jeremy R., Gimble, Frederick S.

“…[Display omitted] •The yeast I-WcaI and I-SceI homing endonucleases are recently diverged homologues that target the same genomic site.•Expression of I-WcaI,…”
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Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae by Gimble, F.S, Thorner, J

“…An unusual protein splicing reaction joins the N-terminal segment (A) and the C-terminal segment (C) of the 119K primary translation product (ABC) of the yeast…”
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Engineering Variants of the I-SceI Homing Endonuclease with Strand-specific and Site-specific DNA-nicking Activity by Niu, Yan, Tenney, Kristen, Li, Hongye, Gimble, Frederick S.

“…The number of strand-specific nicking endonucleases that are currently available for laboratory procedures and applications in vivo is limited, and none is…”
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Evolution of I-SceI Homing Endonucleases with Increased DNA Recognition Site Specificity by Joshi, Rakesh, Ho, Kwok Ki, Tenney, Kristen, Chen, Jui-Hui, Golden, Barbara L., Gimble, Frederick S.

“…Elucidating how homing endonucleases undergo changes in recognition site specificity will facilitate efforts to engineer proteins for gene therapy…”
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Invasion of a multitude of genetic niches by mobile endonuclease genes by Gimble, Frederick S

“…Persistence of a mobile DNA element in a population reflects a balance between the ability of the host to eliminate the element and the ability of the element…”
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Invasion of a multitude of genetic niches by mobile endonuclease genes by Gimble, Frederick S.

“…Abstract Persistence of a mobile DNA element in a population reflects a balance between the ability of the host to eliminate the element and the ability of the…”
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The Crystal Structure of the Gene Targeting Homing Endonuclease I- SceI Reveals the Origins of its Target Site Specificity by Moure, Carmen M, Gimble, Frederick S, Quiocho, Florante A

“…The I- SceI homing endonuclease enhances gene targeting by introducing double-strand breaks at specific chromosomal loci, thereby increasing the recombination…”
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Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway by Moure, Carmen M, Gimble, Frederick S, Quiocho, Florante A

“…I-SceI is a homing endonuclease that specifically cleaves an 18-bp double-stranded DNA. I-SceI exhibits a strong preference for cleaving the bottom strand DNA…”
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Assessing the plasticity of DNA target site recognition of the PI-SceI homing endonuclease using a bacterial two-hybrid selection system by Gimble, Frederick S, Moure, Carmen M, Posey, Karen L

“…The PI-SceI protein from Saccharomyces cerevisiae is a member of the LAGLIDADG family of homing endonucleases that have been used in genomic engineering. To…”
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Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence by Moure, Carmen M, Gimble, Frederick S, Quiocho, Florante A

“…The first X-ray structures of an intein-DNA complex, that of the two-domain homing endonuclease PI-SceI bound to its 36-base pair DNA substrate, have been…”
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Broken Symmetry in Homing Endonucleases by Gimble, Frederick S.

“…Homing DNA endonucleases are highly site-specific enzymes that initiate the transfer of mobile DNA elements. In this issue of Structure, Spiegel et al. report…”
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Evolution of divergent DNA recognition specificities in VDE homing endonucleases from two yeast species by Posey, K.L, Koufopanou, V, Burt, A, Gimble, F.S

“…Homing endonuclease genes (HEGs) are mobile DNA elements that are thought to confer no benefit to their host. They encode site-specific DNA endonucleases that…”
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Protein splicing elements: inteins and exteins — a definition of terms and recommended nomenclature by Perler, Francine B., Davis, Elaine O., Dean, Gary E., Gimble, Frederick S., Jack, William E., Neff, Norma, Noren, Christopher J., Thorner, Jeremy, Belfort, Marlene

“…Several archaeal, eubacterial and eucaryotic genes have been identified with in-frame insertions that are excised at the protein level, not at the RNA level…”
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Degeneration of a homing endonuclease and its target sequence in a wild yeast strain by Gimble, F S

“…Mobile introns and inteins self-propagate by 'homing', a gene conversion process initiated by site-specific homing endonucleases. The VMA intein, which encodes…”
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A bacterial one-hybrid system to isolate homing endonuclease variants with altered DNA target specificities by Joshi, Rakesh, Gimble, Frederick S

“…Chromosomal cleavage near the site of mutations that cause disease can facilitate the targeted repair of the locus. Gene therapy protocols therefore require…”
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Insertion of a Reversible Redox Switch into a Rare-Cutting DNA Endonuclease by Posey, Karen L, Gimble, Frederick S

“…Target sites for homing endonucleases occur infrequently in complex genomes. As a consequence, these enzymes can be used in mammalian systems to introduce…”
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Substitutions in Conserved Dodecapeptide Motifs That Uncouple the DNA Binding and DNA Cleavage Activities of PI-SceI Endonuclease (∗) by Gimble, Frederick S., Stephens, Brian W.

“…The PI-SceI endonuclease from yeast belongs to a protein family whose members contain two conserved dodecapeptide motifs within their primary sequences. The…”
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Amino Acid Residues in Both the Protein Splicing and Endonuclease Domains of the PI-SceI Intein Mediate DNA Binding by He, Z, Crist, M, Yen, H, Duan, X, Quiocho, F A, Gimble, F S

“…A structure-based model describing the interaction of the two-domain PI- Sce I endonuclease with its 31-base pair DNA substrate suggests that the endonuclease…”
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