Search Results - "Gilkes, N R"

Domains in microbial β-1,4-glycanases : sequence conservation, function, and enzyme families by GILKES, N. R, HENRISSAT, B, KILBURN, D. G, MILLER, R. C, WARREN, R. A. J

“…Several types of domain occur in beta-1, 4-glycanases. The best characterized of these are the catalytic domains and the cellulose-binding domains. The domains…”
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Stereoselective hydrolysis catalyzed by related beta-1,4-glucanases and beta-1,4-xylanases by J Gebler, N R Gilkes, M Claeyssens, D B Wilson, P Béguin, W W Wakarchuk, D G Kilburn, R C Miller, Jr, R A Warren, S G Withers

“…Over 80 beta-1,4-glucanases and beta-1,4-xylanases can be classified into one of eight families on the basis of amino acid sequence similarities in their…”
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The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose by Gilkes, N.R. (University of British Columbia, Vancouver, British Columbia, Canada), Jervis, E, Henrissat, B, Tekant, B, Miller, R.C. Jr, Warren, R.A.J, Kilburn, D.G

“…CenA is a bacterial cellulase (beta-1,4-glucanase) comprised of a globular catalytic domain joined to an extended cellulose-binding domain (CBD) by a short…”
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Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease and the effect on catalysis by Gilkes, N R, Warren, R A, Miller, R C, Kilburn, D G

“…An endo-beta-1,4-glucanase (CenA) and an exo-beta-1,4-glucanase (Cex) were prepared from Escherichia coli expressing recombinant DNA of the cellulolytic…”
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Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi by Shen, H, Gilkes, N.R, Kilburn, D.G, Miller, R.C. Jr, Warren, R.A.J

“…The gene cbhB from the cellulolytic bacterium Cellulomonas fimi encodes a polypeptide of 1090 amino acids. Cellobiohydrolase B (CbhB) is 1037 amino acids long,…”
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The binding of Cellulomonas fimi endoglucanase C (CenC) to cellulose and Sephadex is mediated by the N-terminal repeats by Coutinho, J B, Gilkes, N R, Warren, R A, Kilburn, D G, Miller, Jr, R C

“…Endoglucanase C (CenC) from Cellulomonas fimi binds to cellulose and to Sephadex. The enzyme has two contiguous 150-amino-acid repeats (N1 and N2) at its…”
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Glutamic acid 274 is the nucleophile in the active site of a "retaining" exoglucanase from Cellulomonas fimi by TULL, D, WITHERS, S. G, GILKES, N. R, KILBURN, D. G, ANTONY, R, WARREN, R. A. J, AEBERSOLD, R

“…In addition to its known substrate activity with p-nitrophenyl beta-cellobioside, the exoglucanase from Cellulomonas fimi has been shown to utilize substituted…”
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Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta- 1,4-glucanase by Meinke, A, Gilkes, N.R, Kilburn, D.G, Miller, R.C. Jr, Warren, R.A.J

“…Five cellulose-binding polypeptides were detected in Cellulomonas fimi culture supernatants. Two of them are CenA and CenB, endo-beta-1,4-glucanases which have…”
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Deletion of the linker connecting the catalytic and cellulose-binding domains of endoglucanase A (CenA) of Cellulomonas fimi alters its conformation and catalytic activity by H Shen, M Schmuck, I Pilz, N R Gilkes, D G Kilburn, R C Miller, Jr, R A Warren

“…The Pro-Thr box is a linker of 23 amino acids ((PT)4T(PT)7) connecting the catalytic domain and the cellulose-binding domain (CBD) of endoglucanase A (CenA)…”
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Changes in the molecular-size distribution of insoluble celluloses by the action of recombinant Cellulomonas fimi cellulases by KLEMAN-LEYER, K. M, GILKES, N. R, MILLER, R. C, KIRK, T. K

“…Specific patterns of attacks of cotton, bacterial cellulose and bacterial microcrystalline cellulose (BMCC) by recombinant cellulases of Cellulomonas fimi were…”
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Glu280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-beta-1,4-glucanase by Wang, Q, Tull, D, Meinke, A, Gilkes, N R, Warren, R A, Aebersold, R, Withers, S G

“…A new mechanism-based inactivator of beta-1,4-glucanases, 2',4'-dinitrophenyl-2-deoxy-2-fluoro-beta-D-cellobioside, was synthesized and used to trap the…”
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The cellulose-binding domain of endoglucanase A (CenA) from Cellulomonas fimi: evidence for the involvement of tryptophan residues in binding by Din, N, Forsythe, I J, Burtnick, L D, Gilkes, N R, Miller, Jr, R C, Warren, R A, Kilburn, D G

“…Cellulomonas fimi endo-beta-1,4-glucanase A (CenA) contains a discrete N-terminal cellulose-binding domain (CBDCenA). Related CBDs occur in at least 16…”
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Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi by MEINKE, A, BRAUN, C, GILKES, N. R, KILBURN, D. G, MILLER, R. C, WARREN, R. A. J

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Characterization of CenC, an enzyme from Cellulomonas fimi with both endo- and exoglucanase activities by Tomme, P, Kwan, E, Gilkes, N R, Kilburn, D G, Warren, R A

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Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains by Langsford, M.L., Gilkes, N.R., Singh, B., Moser, B., Miller, R.C., Warren, R.A.J., Kilburn, D.G.

“…Glycosylated cellulases from Cellulomonas fimi were compared with their non-glycosylated counterparts synthesized in Escherichia coli from recombinant DNA…”
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Probing the role of tryptophan residues in a cellulose‐binding domain by chemical modification by Bray, Mark R., Gilkes, Neil R., Kilburn, Douglas G., Warren, R. Antony J., Mcintosh, Lawrence P., Johnson, Philip E.

“…The cellulose‐binding domain (CBDCex) of the mixed function glucanase‐xylanase Cex from Cellulomonas fimi contains five tryptophans, two of which are located…”
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Multiple domains in endoglucanase B (CenB) from Cellulomonas fimi: functions and relatedness to domains in other polypeptides by MEINKE, A, GILKES, N. R, KILBURN, D. G, MILLER, R. C, WARREN, R. A. J

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Analysis of Molecular Size Distributions of Cellulose Molecules during Hydrolysis of Cellulose by Recombinant Cellulomonas fimiβ-1,4-Glucanases by STALBRAND, H, MANSFIELD, S. D, SADDLER, J. N, KILBURN, D. G, WARREN, R. A. J, GILKES, N. R

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Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a beta-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II by Meinke, A, Gilkes, N R, Kwan, E, Kilburn, D G, Warren, R A, Miller, Jr, R C

“…The gene cbhA from the cellulolytic bacterium Cellulomonas fimi encodes a protein of 872 amino acids designated cellobiohydrolase A (CbhA). Mature CbhA…”
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Visualization of the adsorption of a bacterial endo-beta-1,4-glucanase and its isolated cellulose-binding domain to crystalline cellulose by Gilkes, N R, Kilburn, D G, Miller, Jr, R C, Warren, R A, Sugiyama, J, Chanzy, H, Henrissat, B

“…Endo-beta-1,4-glucanase A (CenA), a cellulase from the bacterium Cellulomonas fimi, is composed of two domains: a catalytic domain and a cellulose-binding…”
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