Pathogenic Escherichia coli and enteric viruses in biosolids and related top soil improvers in Italy

Pathogenic Escherichia coli and enteric viruses in biosolids and related top soil improvers in Italy

Aims To investigate the presence of genomic traits associated with a set of enteric viruses as well as pathogenic Escherichia coli in top soil improvers (TSI) from Italy. Methods and Results Twenty‐four TSI samples originating from municipal sewage sludges, pig manure, green and household wastes wer...

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Published in:Journal of applied microbiology Vol. 122; no. 1; pp. 239 - 247
Main Authors: Tozzoli, R., Di Bartolo, I., Gigliucci, F., Brambilla, G., Monini, M., Vignolo, E., Caprioli, A., Morabito, S.
Format: Journal Article
Language:English
Published: England Oxford University Press 01-01-2017
Subjects:
Adenoviridae
Animals
E coli
Enterovirus - classification
Enterovirus - genetics
Enterovirus - isolation & purification
environmental
Escherichia coli
Escherichia coli (all potentially pathogenic types)
Escherichia coli - genetics
Escherichia coli - isolation & purification
Hepatitis E virus
Hepeviridae
Humans
Italy
Manure - microbiology
Manure - virology
microbial contamination
Microbiology
Norovirus
Reoviridae
Sewage - microbiology
Sewage - virology
Sludge
sludges
Soil - chemistry
Soil Microbiology
Soils
Swine
Viruses
microbial contamination
Escherichia coli (all potentially pathogenic types)
viruses
sludges
environmental
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Summary:Aims To investigate the presence of genomic traits associated with a set of enteric viruses as well as pathogenic Escherichia coli in top soil improvers (TSI) from Italy. Methods and Results Twenty‐four TSI samples originating from municipal sewage sludges, pig manure, green and household wastes were analysed by real time PCR for the presence of hepatitis E virus (HEV), porcine and human adenovirus (HuAdV), norovirus, rotavirus and diarrhoeagenic E. coli. None of the samples was found positive for HEV or rotavirus. Four samples were positive for the presence of nucleic acids from human norovirus, two of them being also positive for HuAdV. Real time PCR screening gave positive results for many of the virulence genes characteristic of diarrhoeagenic E. coli in 21 samples. These included the verocytotoxin‐coding genes, in some cases associated with intimin‐coding gene, and markers of enteroaggregative, enterotoxigenic and enteroinvasive E. coli. Conclusions These results provide evidence that enteric viruses and pathogenic E. coli may be released into the environment through the use of sludge‐derived TSI. Significance and Impact of the Study The results highlight that the TSI‐related environmental risk for the food chain should be more deeply assessed.
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ISSN:1364-5072
1365-2672
DOI:10.1111/jam.13308