Intracellular pH of symbiotic dinoflagellates

Intracellular pH (pH i ) is likely to play a key role in maintaining the functional success of cnidarian–dinoflagellate symbiosis, yet until now the pH i of the symbiotic dinoflagellates (genus Symbiodinium ) has never been quantified. Flow cytometry was used in conjunction with the ratiometric fluo...

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Bibliographic Details
Published in:	Coral reefs Vol. 32; no. 3; pp. 859 - 863
Main Authors:	Gibbin, E. M., Davy, S. K.
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-09-2013 Springer Springer Nature B.V
Subjects:	Algae Animal and plant ecology Animal, plant and microbial ecology Aquatic ecosystems Biological and medical sciences Biomedical and Life Sciences Cellular biology Cnidaria. Ctenaria Coral reefs Freshwater & Marine Ecology Fundamental and applied biological sciences. Psychology Genotypes Invertebrates Life Sciences Oceanography Protozoa Sea water ecosystems Symbiosis Synecology pH Zooxanthellae Cnidarian–dinoflagellate symbiosis Symbiosis Protozoa Coelenterata Cnidarian-dinoflagellate symbiosis Cnidaria Coral reef Intracellular Invertebrata Symbiodinium
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Summary:	Intracellular pH (pH i ) is likely to play a key role in maintaining the functional success of cnidarian–dinoflagellate symbiosis, yet until now the pH i of the symbiotic dinoflagellates (genus Symbiodinium ) has never been quantified. Flow cytometry was used in conjunction with the ratiometric fluorescent dye BCECF to monitor changes in pH i over a daily light/dark cycle. The pH i of Symbiodinium type B1 freshly isolated from the model sea anemone Aiptasia pulchella was 7.25 ± 0.01 (mean ± SE) in the light and 7.10 ± 0.02 in the dark. A comparable effect of irradiance was seen across a variety of cultured Symbiodinium genotypes (types A1, B1, E1, E2, F1, and F5) which varied between pH i 7.21–7.39 in the light and 7.06–7.14 in the dark. Of note, there was a significant genotypic difference in pH i , irrespective of irradiance.
ISSN:	0722-4028 1432-0975
DOI:	10.1007/s00338-013-1048-7