A thin whole blood smear prepared via pumpless microfluidics

A thin whole blood smear prepared via pumpless microfluidics

Automated generation of a thin blood smear with a pumpless, capillary-driven, microfluidic would overcome the limitations of manually prepared smears and enable potential point-of-care (POC) applications. Herein, this was accomplished with microfluidic design that leveraged an amphiphilic silicone a...

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Bibliographic Details
Published in:Microfluidics and nanofluidics Vol. 25; no. 7
Main Authors: Dogbevi, Kokou S., Ngo, Bryan Khai D., Branan, Kimberly L., Gibbens, Amanda M., Grunlan, Melissa A., Coté, Gerard L.
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-07-2021
Springer Nature B.V
Subjects:
Additives
Analytical Chemistry
Automation
Biomedical Engineering and Bioengineering
Blood
Channels
Dimensions
Engineering
Engineering Fluid Dynamics
Ethylene oxide
Microfluidics
Nanotechnology and Microengineering
Polyethylene oxide
Research Paper
Silicone resins
Silicones
Blood flow control
Poly(ethylene oxide) Surface modifying additive
Pillars
Uniformed blood smear
Pumpless
Microfluidic
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Summary:Automated generation of a thin blood smear with a pumpless, capillary-driven, microfluidic would overcome the limitations of manually prepared smears and enable potential point-of-care (POC) applications. Herein, this was accomplished with microfluidic design that leveraged an amphiphilic silicone and channel pillars. The silicone (Sylgard 184) was combined with a surface-modifying additive (SMA), an amphiphilic poly(ethylene oxide) (PEO)-silane at varying concentrations (3, 5, and 7 wt%). The channels were formed with dimensions of 4.7 µm, 250 µm, and 16 mm (height × width × length, respectively). Pillar sections were added at the inlet, outlet and two interior sections to not only prevent channel collapse but to improve flow and cell distribution. After deposition of blood (0.3, 1, and 2 µL) to the channel inlet, the flow time and flow stop times were recorded and the channels imaged to assess smear uniformity and for automated cell counting. A thin blood smear was generated for microfluidic chips prepared with 5 wt% SMA and provided with 0.3 µL of blood.
ISSN:1613-4982
1613-4990
DOI:10.1007/s10404-021-02457-4