Identification of bacterial microflora in the midgut of the larvae and adult of wild caught Anopheles stephensi: A step toward finding suitable paratransgenesis candidates

[Display omitted] ► Bacterial microflora of the midgut of Anopheles stephensi at both larvae and adult stages were investigated. ► More than 60 isolates/species were identified. ► The bacteria property was tested against the known criteria for nomination of appropriate candidate for paratransgenesis...

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Published in:	Acta tropica Vol. 121; no. 2; pp. 129 - 134
Main Authors:	Chavshin, Ali Reza, Oshaghi, Mohammad Ali, Vatandoost, Hasan, Pourmand, Mohammad Reza, Raeisi, Ahmad, Enayati, Ahmad Ali, Mardani, Nadia, Ghoorchian, Sadigheh
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier B.V 01-02-2012 Elsevier
Subjects:	16S rRNA gene adults Aeromonas Alcaligenes Animals Anopheles - microbiology Anopheles stephensi bacteria Bacteria - classification Bacteria - genetics Bacteria - growth & development Bacteria - isolation & purification Biodiversity Biological and medical sciences Bordetella cell free system Chryseobacterium Cluster Analysis DNA libraries DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Enterobacter Enterococcus Exiguobacterium Female Gastrointestinal Tract - microbiology General aspects genetic engineering Human protozoal diseases Infectious diseases Iran Klebsiella Kocuria Larva - microbiology larvae Malaria Medical sciences Microbacterium Microbiota midgut Molecular Sequence Data Myroides nucleotide sequences Parasitic diseases Paratransgenesis Phylogeny Protozoal diseases Pseudomonas Pseudomonas alcaligenes Rhodococcus Rhodococcus (bacteria) ribosomal RNA RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Shewanella symbionts Iran Microbiota Anopheles stephensi Paratransgenesis Malaria 16S rRNA gene Human Protozoal disease Ribosomal DNA Microflora Identification Parasitosis Infection Bacteriosis Adult Tropical medicine
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Summary:	[Display omitted] ► Bacterial microflora of the midgut of Anopheles stephensi at both larvae and adult stages were investigated. ► More than 60 isolates/species were identified. ► The bacteria property was tested against the known criteria for nomination of appropriate candidate for paratransgenesis. ► Trans-stadial transmission and cell free medium cultivability of Pseudomonas make them proper candidates for paratransgenesis. To describe the midgut microbial diversity and to find the candidate bacteria for the genetic manipulation for the generation of paratransgenic Anopheline mosquitoes refractory to transmission of malaria, the microbiota of wild larvae and adult Anopheles stephensi mosquito midgut from southern Iran was studied using a conventional cell-free culture technique and analysis of a 16S ribosomal RNA (rRNA) gene sequence library. Forty species in 12 genera including seven Gram-negative Myroides, Chryseobacterium, Aeromonas, Pseudomonas, Klebsiella, Enterobacter and Shewanella and five Gram-positive Exiguobacterium, Enterococcus, Kocuria, Microbacterium and Rhodococcus bacteria were identified in the microbiota of the larvae midgut. Analysis of the adult midgut microbiota revealed presence of 25 Gram-negative species in five genera including Pseudomonas, Alcaligenes, Bordetella, Myroides and Aeromonas. Pseudomonas and Exiguobacterium with a frequency of 51% and 14% at the larval stage and Pseudomonas and Aeromonas with a frequency of 54% and 20% at the adult stage were the most common midgut symbionts. Pseudomonas, Aeromonas and Myroides genera have been isolated from both larvae and adult stages indicating possible trans-stadial transmission from larva to adult stage. Fast growth in cheap media, Gram negative, and being dominantly found in both larvae and adult stages, and presence in other malaria vectors makes Pseudomonas as a proper candidate for paratransgenesis of An. stephensi and other malaria vectors.
Bibliography:	http://dx.doi.org/10.1016/j.actatropica.2011.10.015 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0001-706X 1873-6254
DOI:	10.1016/j.actatropica.2011.10.015