Search Results - "Ghisalba, Oreste"

Chemo-enzymatic approaches for the creation of novel chiral building blocks and reagents for pharmaceutical applications by Laumen, Kurt, Kittelmann, Matthias, Ghisalba, Oreste

“…This paper describes three recent examples of innovative biocatalyst applications and technology developments from our laboratory giving access to useful…”
Get full text

Enzyme-assisted preparation of D-tert.-leucine by Laumen, K. (Novartis Pharma AG, Basel (Switzerland)), Ghisalba, O, Auer, K

“…Optically pure D-tert.-leucine was obtained by the enzymatic hydrolysis of ( +- )-7N-acetyl-tert. leucine chloroethyl ester after about 50% conversion, this…”
Get full text

Preparative Enzymatic Synthesis of the Acylglucuronide of Mycophenolic Acid by Kittelmann, Matthias, Rheinegger, Urs, Espigat, Aude, Oberer, Lukas, Aichholz, Reiner, Francotte, Eric, Ghisalba, Oreste

“…The acylglucuronide (3) of mycophenolic acid (1) was enzymatically synthesised on a preparative scale (450 mg substrate) under optimised reaction conditions…”
Get full text

Chemo-enzymatic synthesis of both enantiomers of myo-inositol 1,3,4,5-tetrakisphosphate by Laumen, K. (Novartis Pharma AG, Basel (Switzerland)), Ghisalba, O

“…D-Ins(1,3,4,5)P 4 and unnatural L-Ins(1,3,4,5)P 4 were prepared in gram-quantities from D- and L-2,6-di-O-benzyl-myo-inositol by a chemical phosphorylation and…”
Get full text

Recombinant Lactobacillus leichmannii ribonucleosidetriphosphate reductase as biocatalyst in the preparative synthesis of 2′-deoxyribonucleoside-5′-triphosphates by Brunella, André, Ghisalba, Oreste

“…Recombinant Lactobacillus leichmannii ribonucleosidetriphosphate reductase has been purified and evaluated as a biocatalyst for the preparative synthesis of…”
Get full text

Preparative 2'-reduction of ATP catalyzed by ribonucleotide reductase purified by liquid-liquid extraction by Brunella, A. (Outcomes International, Basel (Switzerland)), Abrantes, M, Ghisalba, O

“…Recombinant Lactobacillus leichmannii ribonucleosidetriphosphate reductase (RTPR, E.C.1.17.4.2) constitutively expressed by E. coli HB101 pSQUIRE has been…”
Get full text

Purification and characterization of dihydrodipicolinate synthase from pea by Dereppe, C. (Ciba-Geigy Ltd., Basel, Switzerland), Bold, G, Ghisalba, O, Ebert, E, Schar, H.P

“…Dihydrodipicolinate synthase (EC 4.2.1.52), the first enzyme unique to lysine biosynthesis in bacteria and higher plants, has been purified to homogeneity from…”
Get full text

Microbial Hydroxylation and Simultaneous Formation of the 4''-O-Methylglucoside of the Tyrosine-Kinase Inhibitor CGP 62706 by Matthias Kittelmann, Lukas Oberer, Wolfgang Blum, Oreste Ghisalba

“…Two fungal strains of Beauveria bassiana, DSM 875 and DSM 1344, hydroxylated CGP 62706, an inhibitor of the EGF-receptor tyrosine kinase, in the C(4') position…”
Get full text

3'-Demethoxy-3'-hydroxystaurosporine, a novel staurosporine analogue produced by a blocked mutant by Hoehn, P, Ghisalba, O, Moerker, T, Peter, H H

“…3'-Demethoxy-3'-hydroxystaurosporine, 1 (CGP 58,546), a novel staurosporine analogue, was isolated from a mutant of Streptomyces longisporoflavus R19 blocked…”
Get more information

Preparation of 4-(4'-hydroxyanilino)-5-anilinophthalimide and 4,5-bis-(4'-hydroxyanilino)-phthalimide by microbial hydroxylation by Weidner, S. (Novartis Pharma AG, Basel (Switzerland)), Goeke, K, Trinks, U, Traxler, P, Ucci-Stoll, K, Ghisalba, O

“…A microbial screening indicated that two fungal strains, Beauveria bassiana DSM 1344=ATCC 7159 and Cunninghamella elegans DSM 1908=ATCC 9245, as well as four…”
Get full text

Preparative-scale Chemo-enzymatic Synthesis of Optically Pure d-myo-Inositol-1-phosphate by Laumen, Kurt, Ghisalba, Oreste

“…d-myo-Inositol-1-phosphate was synthesized by a short and facile route from optically pure 1d-1-acetoxy-4,6-di-O-benzyl-myo-inositol, which was easily obtained…”
Get full text

Preparation of 10,11-Epoxy-carbamazepine and 10,11-Dihydro-10-hydroxy-carbamazepine by Microbial Epoxidation and Hydroxylation by Kittelmann, Matthias, Lattmann, Rene, Ghisalba, Oreste

“…Within a microbial screening two bacterial strains were identified that epoxidize carbamazepine (CBZ) to 10,11-epoxy-CBZ. Ten strains could hydroxylate…”
Get full text

A genetic approach to the biosynthesis of the rifamycin-chromophore in Nocardia mediterranei. IV. Identification of 3-amino-5-hydroxybenzoic acid as a direct precursor of the seven-carbon amino starter-unit by Ghisalba, O, Nüesch, J

“…3-Amino-5-hydroxybenzoic acid was investigated for its ability to induce rifamycin biosynthesis in an appropriate mutant of Nocardia mediterranei and…”
Get more information

Study to the biosynthesis of the rifamycin-chromophore in Nocardia mediterranei by Gygax, D, Ghisalba, O, Treichler, H, Nüesch, J

Get more information

Chemo-enzymatic synthesis of 2'-O-methoxyethyl ribonucleosides using a phosphodiesterase from Serratia marcescens by MARAIS, Guy, GHISALBA, Oreste

“…An enzyme able to cleave the 3',5'-phosphate ring of 2'-methoxyethyl cyclic nucleotides (3',5'-cyclic nucleotide phosphodiesterase, EC 3.1.4.17) from Serratia…”
Get full text

A genetic approach to the biosynthesis of the rifamycin-chromophore in Nocardia mediterranei. V. Studies on the biogenetic origin of 3-substituents by Traxler, P, Ghisalba, O

Get more information

Purification and characterization of N,N‐dimethylformamidase from Pseudomonas DMF 3/3 by SCHÄR, Hans‐Peter, HOLZMANN, Werner, RAMOS TOMBO, Gerardo M., GHISALBA, Oreste

“…The N,N‐dimethylformamide‐hydrolyzing enzyme (DMFase) from Pseudomonas DMF 3/3 has been purified to apparent electrophoretic homogeneity with an overall…”
Get full text

Early intermediates in the biosynthesis of ansamycins. III. Isolation and identification of further 8-deoxyansamycins of the rifamycin-type by Ghisalba, O, Traxler, P, Fuhrer, H, Richter, W J

“…A number of minor compounds were isolated from fermentations of the protorifamycin I producing strain Nocardia mediterranei F 1/24(1,2)) and identified by…”
Get more information

A genetic approach to the biosynthesis of the rifamycin-chromophore in Nocardia mediterranei. I. Isolation and characterization of a pentose-excreting auxotrophic mutant of Nocardia mediterranei with drastically reduced rifamycin production by Ghisalba, O, Nüesch, J

“…The mutant under study, designated A8, is derived from a Nocardia mediterranei strain, N813, which is a high rifamycin B producer. A8 is auxotrophic for…”
Get more information

Enzymatic Synthesis of ?-D-Glucuronides in an Enzyme Membrane Reactor by Ulrike Pfaar, Daniel Gygax, Werner Gertsch, Tammo Winkler, Oreste Ghisalba

“…The production of two O-glucuronides in an enzyme membrane reactor on a 100 to 200 mg scale was examined. The aglycons were conjugated with the co-substrate…”
Get full text