Genetic analysis of nonribosomal peptide synthesis genes (NRPSs) in natural product biosynthesis of the cyanobacterial strains of Lavasan Lake
Introduction: Cyanobacteria are regarded as good candidates for natural pruduct discovery, with applications in pharmaceuticals industry. The majority of bioactive metabolites have either been polyketides, non-ribosomal peptides, or a hybrid of the two. Despite of several worldwide studies on prev...
Saved in:
| Published in: | Zīstʹshināsī-i mīkrūʹurgānīsmʹhā : Biological journal of microorganism Vol. 8; no. 30; pp. 27 - 54 |
|---|---|
| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
University of Isfahan
01-06-2019
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Introduction: Cyanobacteria are regarded as good candidates for natural pruduct discovery, with applications in pharmaceuticals industry. The majority of bioactive metabolites have either been polyketides, non-ribosomal peptides, or a hybrid of the two. Despite of several worldwide studies on prevalence of NRPSs, none of them included Iranian cyanobacteria of Lavasan Lake. Therefore, the aim of this study was to show that the presence of these genes correlates with natural product synthesis.
Materials and methods: In this study, ten cultured fresh water cyanobacteria strains of the Lavasan Lake were identified based on the sequence of 16SrRNA gene. In order to phylogenetic analysis the genes responsible for the production of secondary metabolites, a NRPS PCR was conducted. Bioinformatics software tools were used in order to prediction of peptide compound, amino acid activated and the signature sequences by a specific unknown NRPS A module. Antibiogram bioassays were conducted to detect the presence of antimicrobial effects. Lastly, studied strains have been deposited at the Cyanobacteria Culture Collection (CCC) of herbarium ALBORZ at the Science and Research Branch, Islamic Azad University, Teheran.
Results: the results showed that NRPS genes are presence in all strains. Morover bioinformatics analysis confirmed the type of natural compound, signature sequences and predicted amino acid. In clusterimg of NRPS protein sequences, there was no clear phylogenetic correlation between adenylation domains and activated amino acid and this result emphasize the variety of adenylation domains. Morover the results of antibiogram bioassays showed that there are positive correlation between the presents of those genes and antimicrobial activity.
Discussion and conclusion: Sequence analysis indicates the enzymes encoded by these genes may be responsible for the production of different secondary metabolites, such as antibiotics. The presented results prove that fresh water cyanobacterial of Iran are a promising source to yield chemical and pharmaceutical interesting compounds. |
|---|---|
| ISSN: | 2322-5173 2322-5181 |
| DOI: | 10.22108/bjm.2019.113241.1161 |