Search Results - "Ghadessy, F. J."

Directed Evolution of Polymerase Function by Compartmentalized Self-Replication by Ghadessy, Farid J., Ong, Jennifer L., Holliger, Philipp

“…We describe compartmentalized self-replication (CSR), a strategy for the directed evolution of enzymes, especially polymerases. CSR is based on a simple…”
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Long polyglutamine tracts in the androgen receptor are associated with reduced trans-activation, impaired sperm production, and male infertility by TUT, T. G, GHADESSY, F. J, TRIFIRO, M. A, PINSKY, L, YONG, E. L

“…The X-linked androgen receptor (AR) gene contains two polymorphic trinucleotide repeat segments that code for polyglutamine and polyglycine tracts in the…”
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Towards understanding of laccase-catalysed oxidative oligomerisation of dimeric lignin model compounds by Ramalingam, B., Sana, B., Seayad, J., Ghadessy, F. J., Sullivan, M. B.

“…Laccase-catalysed oligomerisation of dimeric β-O-4 linked lignin model compounds was studied in detail to understand the oligomerisation process by monitoring…”
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Analysis of the transactivation domain of the androgen receptor in patients with male infertility by WANG, Q, GHADESSY, F. J, YONG, E. L

“…Genetic defects of the human androgen receptor (AR) can cause a wide spectrum of androgen insensitivity syndromes (AIS) in XY individuals ranging from…”
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Y chromosome microdeletions, in azoospermic or near-azoospermic subjects, are located in the AZFc (DAZ) subregion by LIOW, S. L, GHADESSY, F. J, NG, S. C, YONG, E. L

“…Submicroscopic deletions of the Y chromosome and polymorphisms of the androgen receptor (AR) gene in the X chromosome have been observed in men with defective…”
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Azoospermia associated with a mutation in the ligand-binding domain of an androgen receptor displaying normal ligand binding, but defective Trans-activation by QI WANG, GHADESSY, F. J, TROUNSON, A, DE KRETSER, D, MCLACHLAN, R, NG, S. C, YONG, E. L

“…Although male infertility affects a significant proportion of couples trying to conceive, the cause of defective spermatogenesis is not known in a large number…”
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Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution by Holliger, Philipp, Ghadessy, Farid J, Ramsay, Nicola, Boudsocq, François, Loakes, David, Brown, Anthony, Iwai, Shigenori, Vaisman, Alexandra, Woodgate, Roger

“…DNA polymerases recognize their substrates with exceptionally high specificity, restricting the use of unnatural nucleotides and the applications they enable…”
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Mutations in the promoter region of the androgen receptor gene are not common in males with idiopathic infertility by Ghadessy, F.J., Liow, S.L., Yong, E.L.

“…Molecular studies on the role of the androgen receptor in male infertility have thus far concentrated solely on exonic regions of the androgen receptor gene…”
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Partial androgen insensitivity and correlations with the predicted three dimensional structure of the androgen receptor ligand-binding domain by Yong, E.L, Tut, T.G, Ghadessy, F.J, Prins, G, Ratnam, S.S

“…Genetic defects of the human androgen receptor (AR) can cause a wide spectrum of androgen insensitivity syndromes (AIS) ranging from phenotypic females in…”
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A genomic region encoding stonefish (Synanceja horrida) stonustoxin beta-subunit contains an intron by Ghadessy, F J, Jeyaseelan, K, Chung, M C, Khoo, H E, Yuen, R

“…The polymerase chain reaction (PCR) has been used to amplify a 1899 base pair fragment from stonefish genomic DNA. A comparison of the translated nucleotide…”
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Oligospermic infertility associated with an androgen receptor mutation that disrupts interdomain and coactivator (TIF2) interactions by Ghadessy, F J, Lim, J, Abdullah, A A, Panet-Raymond, V, Choo, C K, Lumbroso, R, Tut, T G, Gottlieb, B, Pinsky, L, Trifiro, M A, Yong, E L

“…Structural changes in the androgen receptor (AR) are one of the causes of defective spermatogenesis. We screened the AR gene of 173 infertile men with impaired…”
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Human androgen receptor mutation disrupts ternary interactions between ligand, receptor domains, and the coactivator TIF2 (transcription intermediary factor 2) by Lim, J, Ghadessy, F J, Abdullah, A A, Pinsky, L, Trifiro, M, Yong, E L

“…The androgen receptor (AR) is a ligand-dependent X-linked nuclear transcription factor regulating male sexual development and spermatogenesis. The receptor is…”
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Stonustoxin is a novel lethal factor from stonefish (Synanceja horrida) venom. cDNA cloning and characterization by Ghadessy, F J, Chen, D, Kini, R M, Chung, M C, Jeyaseelan, K, Khoo, H E, Yuen, R

“…Stonustoxin (SNTX) is a multifunctional lethal protein isolated from venom elaborated by the stonefish, Synanceja horrida. It comprises two subunits, termed…”
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Synthetic 10FN3-based mono- and bivalent inhibitors of MDM2/X function by Lau, S -Y, Siau, J W, Sobota, R M, Wang, C -I, Zhong, P, Lane, D P, Ghadessy, F J

“…Abstract Engineered non-antibody scaffold proteins constitute a rapidly growing technology for diagnostics and modulation/perturbation of protein function…”
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A novel splice site mutation in the androgen receptor gene results in exon skipping and a non-functional truncated protein by Lim, Joyce, Ghadessy, Farid J, Yong, E.L

“…Mutations of the androgen receptor (AR) gene and protein are associated with complete androgen insensitivity syndromes (CAIS) in individuals with XY genotypes…”
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Towards understanding of laccase-catalysed oxidative oligomerisation of dimeric lignin model compoundsElectronic supplementary information (ESI) available: Complete details of GPC, mass and NMR spectra. See DOI: 10.1039/c6ra26975c by Ramalingam, B, Sana, B, Seayad, J, Ghadessy, F. J, Sullivan, M. B

“…Laccase-catalysed oligomerisation of dimeric β-O-4 linked lignin model compounds was studied in detail to understand the oligomerisation process by monitoring…”
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A "spindle and thread"mechanism unblocks p53 translation N-terminal disorder by Kaldmae, Margit, Vosselman, Thibault, Zhong, Xueying, Lama, Dilraj, Chen, Gefei, Saluri, Mihkel, Kronqvist, Nina, Siau, Jia Wei, Ng, Aik Seng, Ghadessy, Farid J., Sabatier, Pierre, Vojtesek, Borivoj, Sarr, Medoune, Sahin, Cagla, Österlund, Nicklas, Ilag, Leopold L., Vaananen, Venla A., Sedimbi, Saikiran, Arsenian-Henriksson, Marie, Zubarev, Roman A., Nilsson, Lennart, Koeck, Philip J. B., Rising, Anna, Abelein, Axel, Fritz, Nicolas, Johansson, Jan, Lane, David P., Landreh, Michael

“…Disordered proteins pose a major challenge to structural biology. A prominent example is the tumor suppressor p53, whose low expression levels and poor…”
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A “spindle and thread” mechanism unblocks p53 translation by modulating N-terminal disorder by Kaldmäe, Margit, Vosselman, Thibault, Zhong, Xueying, Lama, Dilraj, Chen, Gefei, Saluri, Mihkel, Kronqvist, Nina, Siau, Jia Wei, Ng, Aik Seng, Ghadessy, Farid J., Sabatier, Pierre, Vojtesek, Borivoj, Sarr, Médoune, Sahin, Cagla, Österlund, Nicklas, Ilag, Leopold L., Väänänen, Venla A., Sedimbi, Saikiran, Arsenian-Henriksson, Marie, Zubarev, Roman A., Nilsson, Lennart, Koeck, Philip J.B., Rising, Anna, Abelein, Axel, Fritz, Nicolas, Johansson, Jan, Lane, David P., Landreh, Michael

“…Disordered proteins pose a major challenge to structural biology. A prominent example is the tumor suppressor p53, whose low expression levels and poor…”
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Directed Evolution of p53 Variants with Altered DNA-binding Specificities by In Vitro Compartmentalization by Fen, Chen Xue, Coomber, David W., Lane, David P., Ghadessy, Farid J.

“…The p53 tumour suppressor governs cell fate by differential transactivation of a spectrum of target genes. To further understand how p53 discriminates between…”
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Human Androgen Receptor Mutation Disrupts Ternary Interactions between Ligand, Receptor Domains, and the Coactivator TIF2 (Transcription Intermediary Factor 2) by Lim, Joyce, Ghadessy, Farid J, Abdullah, Abdullah A. R, Pinsky, Leonard, Trifiro, Mark, Yong, E. L

“…The androgen receptor (AR) is a ligand-dependent X-linked nuclear transcription factor regulating male sexual development and spermatogenesis. The receptor is…”
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