Expression of a staphylokinase, a thrombolytic agent in Arabidopsis thaliana

Expression of a staphylokinase, a thrombolytic agent in Arabidopsis thaliana

A gene encoding staphylokinase from Staphylococcus aureus was cloned into the plant transformation binary vector pCAMBIA 1304. The transgene was introduced into the genome of A. thaliana via in planta Agrobacterium tumefaciens–mediated genetic transformation. The presence of the staphylokinase gene...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology Vol. 27; no. 6; pp. 1341 - 1347
Main Authors: Wiktorek-Smagur, Aneta, Hnatuszko-Konka, Katarzyna, Geszberg, Aneta, Łuchniak, Piotr, Kowalczyk, Tomasz, Kononowicz, Andrzej K.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-06-2011
Springer
Springer Nature B.V
Subjects:
Analysis
Applied Microbiology
Bacteria
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Cardiovascular disease
Cloning
Environmental Engineering/Biotechnology
Fundamental and applied biological sciences. Psychology
Genes
Genomes
Gram-positive bacteria
Life Sciences
Microbiology
Original Paper
Plasmids
Proteins
Seeds
Studies
Transgenic animals
Transgenic plants
Staphylokinase
Recombinant proteins
Arabidopsis thaliana
Cruciferae
Dicotyledones
Angiospermae
Agrobacterium tumefaciens
Bacteria
Spermatophyta
Rhizobiaceae
Recombinant protein
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Description
Summary:A gene encoding staphylokinase from Staphylococcus aureus was cloned into the plant transformation binary vector pCAMBIA 1304. The transgene was introduced into the genome of A. thaliana via in planta Agrobacterium tumefaciens–mediated genetic transformation. The presence of the staphylokinase gene was confirmed by PCR in 60% of the investigated plants. The presence of the fusion protein (119 kDa) was confirmed by SDS–PAGE and Western blot analysis in protein extracts from putative transgenics. Furthermore, the amidolytic assay confirmed the activity of SAK in protein extracts in 23 out of 45 transgenic lines of A. thaliana plants.
Bibliography:ObjectType-Article-1
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ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-010-0583-4