Search Results - "Gerlt, JA"

DIVERGENT EVOLUTION OF ENZYMATIC FUNCTION: Mechanistically Diverse Superfamilies and Functionally Distinct Suprafamilies by Gerlt, John A, Babbitt, Patricia C

“…The protein sequence and structure databases are now sufficiently representative that strategies nature uses to evolve new catalytic functions can be…”
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Divergent evolution in the enolase superfamily: the interplay of mechanism and specificity by Gerlt, John A., Babbitt, Patricia C., Rayment, Ivan

“…The members of the mechanistically diverse enolase superfamily catalyze different overall reactions. Each shares a partial reaction in which an active site…”
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The Low Barrier Hydrogen Bond in Enzymatic Catalysis by Cleland, W. Wallace, Frey, Perry A., Gerlt, John A.

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Understanding enzyme superfamilies. Chemistry As the fundamental determinant in the evolution of new catalytic activities by Babbitt, P C, Gerlt, J A

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Evolution of Enzymatic Activities in the Enolase Superfamily:  l-Fuconate Dehydratase from Xanthomonas campestris by Yew, Wen Shan, Fedorov, Alexander A., Fedorov, Elena V., Rakus, John F., Pierce, Richard W., Almo, Steven C., Gerlt, John A.

“…Many members of the mechanistically diverse enolase superfamily have unknown functions. In this report we use both genome (operon) context and screening of a…”
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Mechanistic Diversity in the RuBisCO Superfamily:  The “Enolase” in the Methionine Salvage Pathway in Geobacillus kaustophilus by Imker, Heidi J, Fedorov, Alexander A, Fedorov, Elena V, Almo, Steven C, Gerlt, John A

“…d-Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), the most abundant enzyme, is the paradigm member of the recently recognized mechanistically…”
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The Crotonase Superfamily:  Divergently Related Enzymes That Catalyze Different Reactions Involving Acyl Coenzyme A Thioesters by Holden, Hazel M, Benning, Matthew M, Haller, Toomas, Gerlt, John A

“…Synergistic investigations of the reactions catalyzed by several members of an enzyme superfamily provide a more complete understanding of the relationships…”
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Evolution of Structure and Function in the o-Succinylbenzoate Synthase/ N-Acylamino Acid Racemase Family of the Enolase Superfamily by Glasner, Margaret E., Fayazmanesh, Nima, Chiang, Ranyee A., Sakai, Ayano, Jacobson, Matthew P., Gerlt, John A., Babbitt, Patricia C.

“…Understanding how proteins evolve to provide both exquisite specificity and proficient activity is a fundamental problem in biology that has implications for…”
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Utilization of l-Ascorbate by Escherichia coli K-12: Assignments of Functions to Products of the yjf-sga and yia-sgb Operons by Yew, Wen Shan, Gerlt, John A

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Discovering New Enzymes and Metabolic Pathways:  Conversion of Succinate to Propionate by Escherichia coli by Haller, Toomas, Buckel, Thomas, Rétey, János, Gerlt, John A

“…The Escherichia coli genome encodes seven paralogues of the crotonase (enoyl CoA hydratase) superfamily. Four of these have unknown or uncertain functions;…”
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Evolution of Enzymatic Activities in the Enolase Superfamily:  l-Talarate/Galactarate Dehydratase from Salmonella typhimurium LT2 by Yew, Wen Shan, Fedorov, Alexander A, Fedorov, Elena V, Almo, Steven C, Gerlt, John A

“…We assigned l-talarate dehydratase (TalrD) and galactarate dehydratase (GalrD) functions to a group of orthologous proteins in the mechanistically diverse…”
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Evolution of Enzymatic Activities in the Enolase Superfamily:  N-Succinylamino Acid Racemase and a New Pathway for the Irreversible Conversion of d- to l-Amino Acids by Sakai, Ayano, Xiang, Dao Feng, Xu, Chengfu, Song, Ling, Yew, Wen Shan, Raushel, Frank M, Gerlt, John A

“…Members of the mechanistically diverse enolase superfamily catalyze reactions that are initiated by abstraction of the α-proton of a carboxylate anion to…”
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The crystal structure of benzoylformate decarboxylase at 1.6 Angstrom resolution: Diversity of catalytic residues in thiamin diphosphate-dependent enzymes by Hasson, MS, Muscate, A, McLeish, MJ, Polovnikova, L S, Gerlt, JA, Kenyon, G L, Petsko, G A, Ringe, D

“…The crystal structure of the thiamin diphosphate (ThDP)-dependent enzyme benzoylformate decarboxylase (BFD), the third enzyme in the mandelate pathway of…”
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Evolution of Enzymatic Activities in the Enolase Superfamily:  d-Tartrate Dehydratase from Bradyrhizobium japonicum by Yew, Wen Shan, Fedorov, Alexander A., Fedorov, Elena V., Wood, Bryant McKay, Almo, Steven C., Gerlt, John A.

“…We focus on the assignment of function to and elucidation of structure−function relationships for a member of the mechanistically diverse enolase superfamily…”
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An explanation for rapid enzyme-catalyzed proton abstraction from carbon acids: importance of late transition states in concerted mechanisms by Gerlt, John A, Gassman, Paul G

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The Enolase Superfamily:  A General Strategy for Enzyme-Catalyzed Abstraction of the α-Protons of Carboxylic Acids by Babbitt, Patricia C, Hasson, Miriam S, Wedekind, Joseph E, Palmer, David R. J, Barrett, William C, Reed, George H, Rayment, Ivan, Ringe, Dagmar, Kenyon, George L, Gerlt, John A

“…We have discovered a superfamily of enzymes related by their ability to catalyze the abstraction of the α-proton of a carboxylic acid to form an enolic…”
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Evolution of Enzymatic Activity in the Enolase Superfamily:  Functional Studies of the Promiscuous o-Succinylbenzoate Synthase from Amycolatopsis by Taylor Ringia, Erika A, Garrett, James B, Thoden, James B, Holden, Hazel M, Rayment, Ivan, Gerlt, John A

“…o-Succinylbenzoate synthase (OSBS) from Amycolatopsis, a member of the enolase superfamily, catalyzes the Mn2+-dependent exergonic dehydration of…”
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Evolution of Enzymatic Activities in the Enolase Superfamily:  Functional Assignment of Unknown Proteins in Bacillus subtilis and Escherichia coli as l-Ala-d/l-Glu Epimerases by Schmidt, Dawn M. Z, Hubbard, Brian K, Gerlt, John A

“…The members of the mechanistically diverse enolase superfamily catalyze different overall reactions by using a common catalytic strategy and structural…”
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Evolution of Enzymatic Activities in the Orotidine 5‘-Monophosphate Decarboxylase Suprafamily:  Enhancing the Promiscuous d-arabino-Hex-3-ulose 6-Phosphate Synthase Reaction Catalyzed by 3-Keto-l-gulonate 6-Phosphate Decarboxylase by Yew, Wen Shan, Akana, Julie, Wise, Eric L., Rayment, Ivan, Gerlt, John A.

“…3-Keto-l-gulonate 6-phosphate decarboxylase (KGPDC) and d-arabino-hex-3-ulose 6-phosphate synthase (HPS) are members of the orotidine 5‘-monophosphate…”
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3‘- and 5‘-Strand Cleavage Reactions Catalyzed by the Fpg Protein from Escherichia coli Occur via Successive β- and δ-Elimination Mechanisms, Respectively by Bhagwat, Medha, Gerlt, John A

“…The Fpg protein from Escherichia coli is a multifunctional protein that excises damaged purine bases from DNA to generate aldehydic abasic sites and then…”
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