Changes of fractional anisotropy (FA) and apparent diffusion coefficient (ADC) in the model of experimental acute hydrocephalus in rabbits

Changes of fractional anisotropy (FA) and apparent diffusion coefficient (ADC) in the model of experimental acute hydrocephalus in rabbits

Background To study the integrity of white matter, we investigated the correlation between the changes in neuroradiological and morphological parameters in an animal model of acute obstructive hydrocephalus. Methods Hydrocephalus was induced in New Zealand rabbits ( n  = 10) by stereotactic injectio...

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Published in:Acta neurochirurgica Vol. 157; no. 4; pp. 689 - 698
Main Authors: Shevtsov, Maxim A., Senkevich, Konstantin A., Kim, Alexander V., Gerasimova, Kseniia A., Trofimova, Tatyana N., Kataeva, Galina V., Medvedev, Sviatoslav V., Smirnova, Olga I., Savintseva, Zhanna I., Martynova, Marina G., Bystrova, Olga A., Pitkin, Emil, Yukina, Galina Y., Khachatryan, William A.
Format: Journal Article
Language:English
Published: Vienna Springer Vienna 01-04-2015
Springer Nature B.V
Subjects:
Animals
Anisotropy
Diffusion Tensor Imaging - methods
Disease Models, Animal
Experimental Research - Pediatrics
Hydrocephalus - pathology
Interventional Radiology
Magnetic Resonance Imaging - methods
Male
Medicine
Medicine & Public Health
Minimally Invasive Surgery
Nerve Fibers, Myelinated - pathology
Neurology
Neuroradiology
Neurosurgery
Rabbits
Surgical Orthopedics
White Matter - pathology
Fractional anisotropy
Hydrocephalus
Magnetic resonance imaging
Ventriculomegaly
White matter
Apparent diffusion coefficient
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Summary:Background To study the integrity of white matter, we investigated the correlation between the changes in neuroradiological and morphological parameters in an animal model of acute obstructive hydrocephalus. Methods Hydrocephalus was induced in New Zealand rabbits ( n  = 10) by stereotactic injection of kaolin into the lateral ventricles. Control animals received saline in place of kaolin ( n  = 10). The progression of hydrocephalus was assessed using magnetic resonance imaging. Regional fractional anisotropy (FA) and the apparent diffusion coefficient (ADC) were measured in several white matter regions before and after the infusion of kaolin. Morphology of myelinated nerve fibers as well as of the blood–brain barrier were studied with the help of transmission electron microscopy (TEM) and light microscopy. Results Compared with control animals, kaolin injection into the ventricles resulted in a dramatic increase in ventricular volume with compression of basal cisterns, brain shift and periventricular edema (as observed on magnetic resonance imaging [MRI]). The values of ADC in the periventricular and periaqueductal areas significantly increased in the experimental group ( P  < 0.05). FA decreased by a factor of 2 in the zones of periventricular, periaqueductal white matter and corpus collosum. Histological analysis demonstrated the impairment of the white matter and necrobiotic changes in the cortex. Microsctructural alterations of the myelin fibers were further proved with the help of TEM. Blood–brain barrier ultrastructure assessment showed the loss of its integrity. Conclusions The study demonstrated the correlation of the neuroradiological parameters with morphological changes. The abnormality of the FA and ADC parameters in the obstructive hydrocephalus represents a significant implication for the diagnostics and management of hydrocephalus in patients.
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ISSN:0001-6268
0942-0940
DOI:10.1007/s00701-014-2339-7