Identification and overexpression of human neutrophil α-defensins (human neutrophil peptides 1, 2 and 3) in squamous cell carcinomas of the human tongue

To date, little attention has been paid to the possible role of α-defensins (human neutrophil peptides 1-3), HNP-1, HNP-2 and HNP-3 in innate host defence against tumour invasion. In the current study, using a single-dimensional high pressure liquid chromatography (HPLC) method for peptide separatio...

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Bibliographic Details
Published in:	Oral oncology Vol. 40; no. 2; pp. 139 - 144
Main Authors:	Lundy, Fionnuala T., Orr, David F., Gallagher, James R., Maxwell, Perry, Shaw, Chris, Napier, Seamus S., Gerald Cowan, C., Lamey, Philip-John, Marley, John J.
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-02-2004 Elsevier
Subjects:	Adult alpha-Defensins - metabolism Amino Acid Sequence Biological and medical sciences Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Chromatography, High Pressure Liquid Defensin Female Human Humans Male Medical sciences Middle Aged Molecular Sequence Data Neoplasm Proteins - metabolism Neoplasm Staging Oral cancer Otorhinolaryngology. Stomatology Proteomics Tongue Neoplasms - metabolism Tongue Neoplasms - pathology Tumors Tumour Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology Human OSCC Oral cancer Matrix assisted laser desorption ionisation mass spectrometry MALDI-MS 2D-PAGE 2-dimensional polyacrylamide gel electrophoresis HNP Oral squamous cell carcinoma High pressure liquid chromatography Proteomics Tumour Human defensin Defensin HD HPLC Human neutrophil peptide Squamous cell carcinoma Tongue Peptides Stomatology Granulocyte ENT Malignant tumor Cancerology Oral cavity disease Neutrophil
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Summary:	To date, little attention has been paid to the possible role of α-defensins (human neutrophil peptides 1-3), HNP-1, HNP-2 and HNP-3 in innate host defence against tumour invasion. In the current study, using a single-dimensional high pressure liquid chromatography (HPLC) method for peptide separation, followed by mass spectrometry and amino acid sequencing for identification and quantitation, we report the overexpression of HNP-1, HNP-2 and HNP-3 in squamous cell carcinomas of the human tongue compared with autogenous non-tumour tissue. Using a specific antibody we show that the defensins are abundant in neutrophils infiltrating human oral squamous cell carcinoma tissue. In the context of their previously reported oncolytic activity, our results may imply a role for α-defensins in host defence against oral squamous cell carcinoma.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1368-8375 1879-0593
DOI:	10.1016/S1368-8375(03)00142-8