Lack of natural killer cell precursors in fetal liver of Ikaros knockout mutant mice

Lack of natural killer cell precursors in fetal liver of Ikaros knockout mutant mice

The role of Ikaros in early stages of natural killer (NK) cell differentiation was investigated using an in vitro system that promotes proliferation and differentiation of NK cell precursors into mature NK1.1+ cells. Day 14.5 fetal liver cells from mice, either homozygous for Ikaros Null or dominant...

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Bibliographic Details
Published in:Natural immunity Vol. 16; no. 4; p. 137
Main Authors: Boggs, S S, Trevisan, M, Patrene, K, Geogopoulos, K
Format: Journal Article
Language:English
Published: Switzerland 01-01-1998
Subjects:
Animals
DNA-Binding Proteins
Female
Fetus - cytology
Fetus - immunology
Hematopoietic Stem Cells - immunology
Heterozygote
Homozygote
Ikaros Transcription Factor
In Vitro Techniques
Killer Cells, Natural - immunology
Liver - cytology
Liver - immunology
Mice
Mice, Knockout
Pregnancy
Transcription Factors - deficiency
Transcription Factors - genetics
Transcription Factors - immunology
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Summary:The role of Ikaros in early stages of natural killer (NK) cell differentiation was investigated using an in vitro system that promotes proliferation and differentiation of NK cell precursors into mature NK1.1+ cells. Day 14.5 fetal liver cells from mice, either homozygous for Ikaros Null or dominant negative (DN) mutations, had severe 55- to 79-fold reductions in functional NK cell precursors. Although there was no statistically significant difference between values for +/+ and +/- Null mice, the mean precursor frequency for DN mutant (+/-) mice was significantly above that for DN -/- mice and below that for DN +/+ mice. The NK activity values for cells generated from the NK cell precursors followed the same respective relationships found for NK cell precursor frequencies. These data suggest that the deficiency of mature NK cells in Ikaros mutant mice is related to lack of functional precursors.
ISSN:1018-8916
DOI:10.1159/000069438