Molecular surveillance of rotavirus strains circulating in Yaoundé, Cameroon, September 2007–December 2012

Molecular surveillance of rotavirus strains circulating in Yaoundé, Cameroon, September 2007–December 2012

•Molecular surveillance of rotavirus strains in Yaoundé, Cameroon 2007–2012.•Forty percent of children under 5years were positive for rotavirus antigen by EIA testing.•The highest detection rates were observed in children aged 3–5 months (50%).•A large variety of rotavirus strains were identified (3...

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Published in:Infection, genetics and evolution Vol. 28; pp. 470 - 475
Main Authors: Boula, Angeline, Waku-Kouomou, Diane, Njiki Kinkela, Mina, Esona, Mathew D., Kemajou, Grace, Mekontso, David, Seheri, Mapaseka, Ngum Ndze, Valantine, Emah, Irene, Ela, Serge, Dahl, Benjamin A., Kobela, Marie, Cavallaro, Kathleen F., Etoundi Mballa, Georges Alain, Genstch, Jon R., Bowen, Michael D., Koki Ndombo, Paul
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-12-2014
Subjects:
Age Distribution
Cameroon
Cameroon - epidemiology
Child, Preschool
Feces - virology
Gastroenteritis - epidemiology
Gastroenteritis - history
Gastroenteritis - virology
Genotype
History, 21st Century
Humans
Infant
Infant, Newborn
Molecular epidemiology
Population Surveillance
Rotavirus
Rotavirus - classification
Rotavirus - genetics
Rotavirus Infections - epidemiology
Rotavirus Infections - history
Rotavirus Infections - virology
Seasons
Spatio-Temporal Analysis
Surveillance
Cameroon
Molecular epidemiology
Cameroon
Rotavirus
Surveillance
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Summary:•Molecular surveillance of rotavirus strains in Yaoundé, Cameroon 2007–2012.•Forty percent of children under 5years were positive for rotavirus antigen by EIA testing.•The highest detection rates were observed in children aged 3–5 months (50%).•A large variety of rotavirus strains were identified (33 G/P combinations).•The frequencies of the individual genotypes varied from year to year. Rotavirus is the most common cause of severe diarrheal disease in children under 5years of age worldwide. The World Health Organization (WHO) estimated that 453,000 rotavirus-attributable deaths occur annually. Through the WHO, the Rotavirus Sentinel Surveillance Program was established in Cameroon in September 2007 with the Mother and Child Center (MCC) in Yaoundé playing the role of sentinel site and national laboratory for this program. The objectives of this surveillance were to assess the rotavirus disease burden and collect baseline information on rotavirus strains circulating in Cameroon. Diarrheal stool samples were collected in a pediatric hospital from children under 5, using the WHO case definition for rotavirus diarrhea. Antigen detection of rotavirus was performed by using an enzyme immunoassay (EIA). The genotypic characterization was performed using multiplexed semi-nested reverse transcription-polymerase chain reaction (RT-PCR) assays. Between September 2007 and December 2012, 2444 stool samples were received at the MCC laboratory for rotavirus antigen detection, of which 999 (41%) were EIA positive. Among EIA positive samples 898 were genotyped. Genotype prevalence varied each year. Genotype G9P[8] was the dominant type during 2007 (32%) and 2008 (24%), genotype G3P[6] predominated in 2010 (36%) and 2011 (25%), and G1P[8] was predominant in 2012 (44%). The findings showed that the rotavirus disease burden is high and there is a broad range of rotavirus strains circulating in Yaoundé. These data will help measure the impact of vaccination in the future.
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ISSN:1567-1348
1567-7257
DOI:10.1016/j.meegid.2014.08.019