Hopanoids, like sterols, modulate dynamics, compaction, phase segregation and permeability of membranes

In recent years, hopanoids, a group of pentacyclic compounds found in bacterial membranes, are in the spotlight since it was proposed that they induce order in lipid membranes in a similar way cholesterol do in eukaryotes, despite their structural differences. We studied here whether diplopterol (an...

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Published in:	Biochimica et biophysica acta. Biomembranes Vol. 1861; no. 12; p. 183060
Main Authors:	Mangiarotti, Agustín, Genovese, Darío M., Naumann, Christoph A., Monti, Mariela R., Wilke, Natalia
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-12-2019
Subjects:	Bacterial survival Cell Membrane - chemistry Cell Membrane - physiology Cell Membrane Permeability - drug effects Cell Membrane Permeability - physiology Diplopterol Lipid Bilayers - chemistry Liquid-ordered phase Membrane Lipids - chemistry Membrane Lipids - physiology Membrane permeability Membrane rheology Membranes - chemistry Membranes - physiology Models, Biological Permeability Phospholipids - chemistry Phospholipids - physiology Pseudomonadaceae - metabolism Sterols - chemistry Triterpenes - metabolism Triterpenes - pharmacology Bacterial survival Membrane rheology Liquid-ordered phase Membrane permeability Diplopterol
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Summary:	In recent years, hopanoids, a group of pentacyclic compounds found in bacterial membranes, are in the spotlight since it was proposed that they induce order in lipid membranes in a similar way cholesterol do in eukaryotes, despite their structural differences. We studied here whether diplopterol (an abundant hopanoid) promoted similar effects on model membranes as sterols do. We analyzed the compaction, dynamics, phase segregation, permeability and compressibility of model membranes containing diplopterol, and compared with those containing sterols from animals, plants and fungi. We also tested the effect that the incubation with diplopterol had on hopanoid-lacking bacteria. Our results show that diplopterol induces phase segregation, increases lipid compaction, and decreases permeability on phospholipid membranes, while retaining membrane fluidity and compressibility. Furthermore, the exposition to this hopanoid decreases the permeability of the opportunistic pathogen Pseudomonas aeruginosa and increases the resistance to antibiotics. All effects promoted by diplopterol were similar to those generated by the sterols. Our observations add information on the functional significance of hopanoids as molecules that play an important role in membrane organization and dynamics in model membranes and in a bacterial system. [Display omitted] •The hopanoid diplopterol reduces membrane permeability.•Diplopterol condense monolayers without affecting fluidity and compressibility.•Diplopterol increases resistance to antibiotics of P. aeruginosa.•This hopanoid induces liquid-liquid phase segregation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0005-2736 1879-2642
DOI:	10.1016/j.bbamem.2019.183060