Search Results - "Geierstanger, Bernhard H."

Facile System for Encoding Unnatural Amino Acids in Mammalian Cells by Chen, Peng R, Groff, Dan, Guo, Jiantao, Ou, Weijia, Cellitti, Susan, Geierstanger, Bernhard H, Schultz, Peter G

“…A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli…”
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The Pungency of Garlic: Activation of TRPA1 and TRPV1 in Response to Allicin by Macpherson, Lindsey J., Geierstanger, Bernhard H., Viswanath, Veena, Bandell, Michael, Eid, Samer R., Hwang, SunWook, Patapoutian, Ardem

“…Garlic’s pungent flavor has made it a popular ingredient in cuisines around the world and throughout history. Garlic’s health benefits have been elevated from…”
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Discovery and multimerization of cross-reactive single-domain antibodies against SARS-like viruses to enhance potency and address emerging SARS-CoV-2 variants by Hollingsworth, Scott A., Noland, Cameron L., Vroom, Karin, Saha, Anasuya, Sam, Miranda, Gao, Qinshan, Zhou, Haihong, Grandy, David U., Singh, Sujata, Wen, Zhiyun, Warren, Christopher, Ma, Xiaohong Shirley, Malashock, Daniel, Galli, Jennifer, Go, Gwenny, Eddins, Michael, Mayhood, Todd, Sathiyamoorthy, Karthik, Fridman, Arthur, Raoufi, Fahimeh, Gomez-Llorente, Yacob, Patridge, Andrea, Tang, Yinyan, Chen, Shi-Juan, Bailly, Marc, Ji, Chengjie, Kingsley, Laura J., Cheng, Alan C., Geierstanger, Bernhard H., Gorman, Daniel M., Zhang, Lan, Pande, Kalyan

“…Coronaviruses have been the causative agent of three epidemics and pandemics in the past two decades, including the ongoing COVID-19 pandemic. A…”
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Efficient Preparation of Site-Specific Antibody–Drug Conjugates Using Phosphopantetheinyl Transferases by Grünewald, Jan, Klock, Heath E, Cellitti, Susan E, Bursulaya, Badry, McMullan, Daniel, Jones, David H, Chiu, Hsien-Po, Wang, Xing, Patterson, Paula, Zhou, Huanfang, Vance, Julie, Nigoghossian, Edward, Tong, Hung, Daniel, Dylan, Mallet, William, Ou, Weijia, Uno, Tetsuo, Brock, Ansgar, Lesley, Scott A, Geierstanger, Bernhard H

“…Post-translational modification catalyzed by phosphopantetheinyl transferases (PPTases) has previously been used to site-specifically label proteins with…”
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In vivo Incorporation of Unnatural Amino Acids to Probe Structure, Dynamics, and Ligand Binding in a Large Protein by Nuclear Magnetic Resonance Spectroscopy by Cellitti, Susan E, Jones, David H, Lagpacan, Leanna, Hao, Xueshi, Zhang, Qiong, Hu, Huiyong, Brittain, Scott M, Brinker, Achim, Caldwell, Jeremy, Bursulaya, Badry, Spraggon, Glen, Brock, Ansgar, Ryu, Youngha, Uno, Tetsuo, Schultz, Peter G, Geierstanger, Bernhard H

“…In vivo incorporation of isotopically labeled unnatural amino acids into large proteins drastically reduces the complexity of nuclear magnetic resonance (NMR)…”
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Structure of Ddn, the Deazaflavin-Dependent Nitroreductase from Mycobacterium tuberculosis Involved in Bioreductive Activation of PA-824 by Cellitti, Susan E., Shaffer, Jennifer, Jones, David H., Mukherjee, Tathagata, Gurumurthy, Meera, Bursulaya, Badry, Boshoff, Helena I., Choi, Inhee, Nayyar, Amit, Lee, Yong Sok, Cherian, Joseph, Niyomrattanakit, Pornwaratt, Dick, Thomas, Manjunatha, Ujjini H., Barry, Clifton E., Spraggon, Glen, Geierstanger, Bernhard H.

“…Tuberculosis continues to be a global health threat, making bicyclic nitroimidazoles an important new class of therapeutics. A deazaflavin-dependent…”
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Hexamerization of Anti-SARS CoV IgG1 Antibodies Improves Neutralization Capacity by Pande, Kalyan, Hollingsworth, Scott A, Sam, Miranda, Gao, Qinshan, Singh, Sujata, Saha, Anasuya, Vroom, Karin, Ma, Xiaohong Shirley, Brazell, Tres, Gorman, Dan, Chen, Shi-Juan, Raoufi, Fahimeh, Bailly, Marc, Grandy, David, Sathiyamoorthy, Karthik, Zhang, Lan, Thompson, Rob, Cheng, Alan C, Fayadat-Dilman, Laurence, Geierstanger, Bernhard H, Kingsley, Laura J

“…The SARS-CoV-2 pandemic and particularly the emerging variants have deepened the need for widely available therapeutic options. We have demonstrated that…”
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Loss of CD4 T-cell-dependent tolerance to proteins with modified amino acids by Gauba, Varun, Grünewald, Jan, Gorney, Vanessa, Deaton, Lisa M, Kang, Mingchao, Bursulaya, Badry, Ou, Weijia, Lerner, Richard A, Schmedt, Christian, Geierstanger, Bernhard H, Schultz, Peter G, Ramirez-Montagut, Teresa

“…The site-specific incorporation of the unnatural amino acid p-nitrophenylalanine (pNO₂Phe) into autologous proteins overcomes self-tolerance and induces a…”
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Multiplexed sandwich assays in microarray format by Nielsen, Ulrik B, Geierstanger, Bernhard H

“…Antibody arrays have evolved into powerful tools for quantifying proteins and qualifying their state of activation in complex biological samples. This level of…”
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Site-specific protein modifications through pyrroline-carboxy-lysine residues by Ou, Weijia, Uno, Tetsuo, Chiu, Hsien-Po, Grünewald, Jan, Cellitti, Susan E, Crossgrove, Tiffany, Hao, Xueshi, Fan, Qian, Quinn, Lisa L, Patterson, Paula, Okach, Linda, Jones, David H, Lesley, Scott A, Brock, Ansgar, Geierstanger, Bernhard H

“…Pyrroline-carboxy-lysine (Pcl) is a demethylated form of pyrrolysine that is generated by the pyrrolysine biosynthetic enzymes when the growth media is…”
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Efforts toward Expansion of the Genetic Alphabet:  Structure and Replication of Unnatural Base Pairs by Matsuda, Shigeo, Fillo, Jeremiah D, Henry, Allison A, Rai, Priyamrada, Wilkens, Steven J, Dwyer, Tammy J, Geierstanger, Bernhard H, Wemmer, David E, Schultz, Peter G, Spraggon, Glen, Romesberg, Floyd E

“…Expansion of the genetic alphabet has been a long-time goal of chemical biology. A third DNA base pair that is stable and replicable would have a great number…”
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Immunoassay and antibody microarray analysis of the HUPO Plasma Proteome Project reference specimens: Systematic variation between sample types and calibration of mass spectrometry data by Haab, Brian B., Geierstanger, Bernhard H., Michailidis, George, Vitzthum, Frank, Forrester, Sara, Okon, Ryan, Saviranta, Petri, Brinker, Achim, Sorette, Martin, Perlee, Lorah, Suresh, Shubha, Drwal, Garry, Adkins, Joshua N., Omenn, Gilbert S.

“…Four different immunoassay and antibody microarray methods performed at four different sites were used to measure the levels of a broad range of proteins (N =…”
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D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine by Cellitti, Susan E, Ou, Weijia, Chiu, Hsien-Po, Grünewald, Jan, Jones, David H, Hao, Xueshi, Fan, Qian, Quinn, Lisa L, Ng, Kenneth, Anfora, Andrew T, Lesley, Scott A, Uno, Tetsuo, Brock, Ansgar, Geierstanger, Bernhard H

“…D -Ornithine has been used as a supplement under the assumption that it increases production of pyrrolysine-containing proteins, but detailed characterization…”
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Evaluating Sandwich Immunoassays in Microarray Format in Terms of the Ambient Analyte Regime by Saviranta, Petri, Okon, Ryan, Brinker, Achim, Warashina, Masaki, Eppinger, Joerg, Geierstanger, Bernhard H

“…Conceptionally, antibody microarrays are simply multiplexed sandwich immunoassays in a miniaturized format. However, from the amounts of capture antibodies…”
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Efforts To Expand the Genetic Alphabet:  Identification of a Replicable Unnatural DNA Self-Pair by Henry, Allison A, Olsen, Anne Goldbech, Matsuda, Shigeo, Yu, Chengzhi, Geierstanger, Bernhard H, Romesberg, Floyd E

“…Six unnatural nucleotides featuring fluorine-substituted phenyl nucleobase analogues have been synthesized, incorporated into DNA, and characterized in terms…”
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Structure of Ddn, the Deazaflavin-Dependent Nitroreductase from Mycobacterium tuberculosis Involved in Bioreductive Activation of PA-824 by Cellitti, Susan E., Shaffer, Jennifer, Jones, David H., Mukherjee, Tathagata, Gurumurthy, Meera, Bursulaya, Badry, Boshoff, Helena I., Choi, Inhee, Nayyar, Amit, Lee, Yong Sok, Cherian, Joseph, Niyomrattanakit, Pornwaratt, Dick, Thomas, Manjunatha, Ujjini H., Barry, Clifton E., Spraggon, Glen, Geierstanger, Bernhard H.

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Simultaneous Purification and Site-Specific Modification of Pyrroline-Carboxy-Lysine Proteins by Chiu, Hsien-Po, Grünewald, Jan, Hao, Xueshi, Brock, Ansgar, Okach, Linda, Uno, Tetsuo, Geierstanger, Bernhard H.

“…Sticky residue: Pyrroline‐carboxy‐lysine (Pcl) can be readily incorporated into proteins expressed in E. coli and mammalian cells by using the pyrrolysyl…”
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Solution Structure of a DNA Duplex Containing a Guanine−Difluorotoluene Pair:  A Wobble Pair without Hydrogen Bonding? by Pfaff, Danielle A, Clarke, Kristine M, Parr, Timothy A, Cole, Joanna M, Geierstanger, Bernhard H, Tahmassebi, Deborah C, Dwyer, Tammy J

“…The incorporation of synthetic nucleoside analogues into DNA duplexes provides a unique opportunity to probe both structure and function of nucleic acids. We…”
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Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation by Grünewald, Jan, Brock, Ansgar, Geierstanger, Bernhard H

“…4'-Phosphopantetheinyl transferases (PPTases) have been employed by researchers as versatile biocatalysts for the site-specific modification of numerous…”
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Control of protein phosphorylation with a genetically encoded photocaged amino acid by Schultz, Peter G, Lemke, Edward A, Summerer, Daniel, Geierstanger, Bernhard H, Brittain, Scott M

“…We genetically encoded the photocaged amino acid 4,5-dimethoxy-2-nitrobenzylserine (DMNB-Ser, 1) in Saccharomyces cerevisiae in response to the amber nonsense…”
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