A high throughput functional expression assay system for a defence gene conferring transgenic resistance on rice against the sheath blight pathogen, Rhizoctonia solani

Should fruits of transgenic research on engineering resistance against crop diseases reach needy farmers, such resistance needs to be characterised thoroughly. Assessment of transgenic resistance conferred by over-expression of defence genes under field conditions or in greenhouse is a time-consumin...

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Published in:	Plant science (Limerick) Vol. 165; no. 5; pp. 969 - 976
Main Authors:	Kumar, Krish K, Poovannan, Kandasami, Nandakumar, Rangaraj, Thamilarasi, Kandasamy, Geetha, Chinnaswami, Jayashree, Nirmalkumar, Kokiladevi, Easwaran, Raja, Joseph A.J, Samiyappan, Ramasamy, Sudhakar, Durailagaraja, Balasubramanian, Ponnuswami
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier Ireland Ltd 01-11-2003 Elsevier Science
Subjects:	Agrobacterium-mediated transformation Agronomy. Soil science and plant productions Biological and medical sciences blight Chitinase Defence genes Disease resistance Functional assessment Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants grain crops Oryza sativa Pest resistance plant pathogenic fungi Plant pathogens plant proteins resistance mechanisms Rhizoctonia solani rice rice sheath blight Sheath blight soil fungi Thanatephorus cucumeris transgenic plants Transgenic rice Varietal selection. Specialized plant breeding, plant breeding aims Chitinase Disease resistance Agrobacterium-mediated transformation Functional assessment Sheath blight Rhizoctonia solani Defence genes Transgenic rice Monocotyledones Plant pathogen Sheath blight: Rhizoctonia solani Cereal crop Fungus resistance Fungi Oryza sativa Gene Gramineae Angiospermae Defense mechanism Spermatophyta Transgenic plant Fungi Imperfecti Thallophyta
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Summary:	Should fruits of transgenic research on engineering resistance against crop diseases reach needy farmers, such resistance needs to be characterised thoroughly. Assessment of transgenic resistance conferred by over-expression of defence genes under field conditions or in greenhouse is a time-consuming process. It becomes often necessary, though laborious, to screen a huge population of putative transgenic lines for their resistance against a target disease. Most standard evaluation systems (SES) available for individual plant–pathogen interactions are applicable only to field conditions, and there has been a constant need for evolving a rapid laboratory method for assessment of efficacy of defence genes deployed against plant diseases. In this paper, we describe a high throughput, yet simple method for functional assessment of a rice chitinase gene chi11 known to confer resistance against Rhizoctonia solani, the rice sheath blight (ShB) pathogen. Accuracy of this method as compared with a SES already available for assessing field performance of test-varieties against ShB is discussed.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0168-9452 1873-2259
DOI:	10.1016/S0168-9452(03)00271-1