Search Results - "Geertsma, Eric R."

Conformation space of a heterodimeric ABC exporter under turnover conditions by Hofmann, Susanne, Januliene, Dovile, Mehdipour, Ahmad R., Thomas, Christoph, Stefan, Erich, Brüchert, Stefan, Kuhn, Benedikt T., Geertsma, Eric R., Hummer, Gerhard, Tampé, Robert, Moeller, Arne

“…Cryo-electron microscopy (cryo-EM) has the capacity to capture molecular machines in action 1 – 3 . ATP-binding cassette (ABC) exporters are highly dynamic…”
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A Versatile and Efficient High-Throughput Cloning Tool for Structural Biology by Geertsma, Eric R, Dutzler, Raimund

“…Methods for the cloning of large numbers of open reading frames into expression vectors are of critical importance for challenging structural biology projects…”
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AcrB: a mean, keen, drug efflux machine by Kobylka, Jessica, Kuth, Miriam S., Müller, Reinke T., Geertsma, Eric R., Pos, Klaas M.

“…Gram‐negative bacteria are intrinsically resistant against cytotoxic substances by means of their outer membrane and a network of multidrug efflux systems,…”
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Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport by Ehrnstorfer, Ines A, Geertsma, Eric R, Pardon, Els, Steyaert, Jan, Dutzler, Raimund

“…The X-ray crystal structure of ScaDMT, a bacterial member of the solute carrier 11 transporter family, identifies conserved residues within the…”
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Generation of synthetic nanobodies against delicate proteins by Zimmermann, Iwan, Egloff, Pascal, Hutter, Cedric A. J., Kuhn, Benedikt T., Bräuer, Philipp, Newstead, Simon, Dawson, Roger J. P., Geertsma, Eric R., Seeger, Markus A.

“…Here, we provide a protocol to generate synthetic nanobodies, known as sybodies, against any purified protein or protein complex within a 3-week period. Unlike…”
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Structural basis for functional interactions in dimers of SLC26 transporters by Chang, Yung-Ning, Jaumann, Eva A., Reichel, Katrin, Hartmann, Julia, Oliver, Dominik, Hummer, Gerhard, Joseph, Benesh, Geertsma, Eric R.

“…The SLC26 family of transporters maintains anion equilibria in all kingdoms of life. The family shares a 7 + 7 transmembrane segments inverted repeat…”
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Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family by Geertsma, Eric R, Chang, Yung-Ning, Shaik, Farooque R, Neldner, Yvonne, Pardon, Els, Steyaert, Jan, Dutzler, Raimund

“…SLC26 membrane proteins constitute a large family of anion transporters with diverse functions. The first structure of a full-length SLC26 transporter now…”
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Synthetic single domain antibodies for the conformational trapping of membrane proteins by Zimmermann, Iwan, Egloff, Pascal, Hutter, Cedric Aj, Arnold, Fabian M, Stohler, Peter, Bocquet, Nicolas, Hug, Melanie N, Huber, Sylwia, Siegrist, Martin, Hetemann, Lisa, Gera, Jennifer, Gmür, Samira, Spies, Peter, Gygax, Daniel, Geertsma, Eric R, Dawson, Roger Jp, Seeger, Markus A

“…Mechanistic and structural studies of membrane proteins require their stabilization in specific conformations. Single domain antibodies are potent reagents for…”
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Interdomain-linkers control conformational transitions in the SLC23 elevator transporter UraA by Kuhn, Benedikt T., Zöller, Jonathan, Zimmermann, Iwan, Gemeinhardt, Tim, Özkul, Dogukan H., Langer, Julian D., Seeger, Markus A., Geertsma, Eric R.

“…Uptake of nucleobases and ascorbate is an essential process in all living organisms mediated by SLC23 transport proteins. These transmembrane carriers operate…”
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The novel class of seven transmembrane segment inverted repeat carriers by Chang, Yung-Ning, Geertsma, Eric R

“…Solute carriers from the SLC4, SLC23, and SLC26 families are involved in pH regulation, vitamin C transport and ion homeostasis. While these families do not…”
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Quality control of overexpressed membrane proteins by Geertsma, Eric R, Groeneveld, Maarten, Slotboom, Dirk-Jan, Poolman, Bert

“…Overexpression of membrane proteins in Escherichia coli frequently leads to the formation of aggregates or inclusion bodies, which is undesirable for most…”
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FX cloning: a simple and robust high-throughput cloning method for protein expression by Geertsma, Eric R

“…The immense amount of gene sequences available nowadays allows scientist to screen broadly for extraordinary proteins. Reliable cloning tools that allow the…”
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Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport by Schenck, Stephan, Kunz, Laura, Sahlender, Daniela, Pardon, Els, Geertsma, Eric R, Savtchouk, Iaroslav, Suzuki, Toshiharu, Neldner, Yvonne, Štefanić, Saša, Steyaert, Jan, Volterra, Andrea, Dutzler, Raimund

“…The uptake of glutamate by synaptic vesicles is mediated by vesicular glutamate transporters (VGLUTs). The central role of these transporters in excitatory…”
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Membrane reconstitution of ABC transporters and assays of translocator function by Poolman, Bert, Geertsma, Eric R, Nik Mahmood, N A B, Schuurman-Wolters, Gea K

“…In this protocol, we describe a procedure for incorporating ATP-binding cassette (ABC) transporters into large unilamellar vesicles (LUVs) and assays to…”
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Selection of nanobodies with broad neutralizing potential against primary HIV-1 strains using soluble subtype C gp140 envelope trimers by Koch, Kathrin, Kalusche, Sarah, Torres, Jonathan L., Stanfield, Robyn L., Danquah, Welbeck, Khazanehdari, Kamal, von Briesen, Hagen, Geertsma, Eric R., Wilson, Ian A., Wernery, Ulrich, Koch-Nolte, Friedrich, Ward, Andrew B., Dietrich, Ursula

“…Broadly neutralizing antibodies (bnAbs) against HIV-1 protect from infection and reduce viral load upon therapeutic applications. However no vaccine was able…”
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Bicistronic mRNAs to Enhance Membrane Protein Overexpression by Marino, Jacopo, Hohl, Michael, Seeger, Markus A., Zerbe, Oliver, Geertsma, Eric R.

“…Functional overexpression of membrane proteins is essential for their structural and functional characterization. However, functional overexpression is often…”
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substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter by Mulligan, Christopher, Geertsma, Eric R, Severi, Emmanuele, Kelly, David J, Poolman, Bert, Thomas, Gavin H

“…Substrate-binding protein-dependent secondary transporters are widespread in prokaryotes and are represented most frequently by members of the tripartite…”
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Flavin Binding to the High Affinity Riboflavin Transporter RibU by Duurkens, Ria H., Tol, Menno B., Geertsma, Eric R., Permentier, Hjalmar P., Slotboom, Dirk Jan

“…The first biochemical and spectroscopic characterization of a purified membrane transporter for riboflavin (vitamin B2) is presented. The riboflavin…”
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Uniport, Not Proton-Symport, in a Non-Mammalian SLC23 Transporter by Holzhüter, Katharina, Geertsma, Eric R.

“…[Display omitted] •Establishment of the first in vitro transport assay for SLC23 transporters.•Discovery of uniport instead of proton-symport in a…”
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The response of Lactococcus lactis to membrane protein production by Marreddy, Ravi K R, Pinto, Joao P C, Wolters, Justina C, Geertsma, Eric R, Fusetti, Fabrizia, Permentier, Hjalmar P, Kuipers, Oscar P, Kok, Jan, Poolman, Bert

“…The biogenesis of membrane proteins is more complex than that of water-soluble proteins, and recombinant expression of membrane proteins in functional form and…”
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