Spatial and temporal regulation of DNA fragmentation in the aleurone of germinating barley

Spatial and temporal regulation of DNA fragmentation in the aleurone of germinating barley

During germination of barley grains, the appearance of DNA fragmentation started in aleurone cells near the embryo and extended to the distal end in a timedependent manner. DNA fragmentation was demonstrated to occur only after the expression of a-amylase mRNA in the aleurone layer. In addition, cel...

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Bibliographic Details
Published in:Journal of experimental botany Vol. 49; no. 325; pp. 1293 - 1301
Main Authors: Wang, Mei, Oppedijk, Berry J., Caspers, Martien P.M., Lamers, Gerda E.M., Boot, Marit J., Geerlings, Dave N.G., Bakhuizen, Bob, Meijer, Annemarie H., Duijn, Bert Van
Format: Journal Article
Language:English
Published: Oxford Oxford University Press 01-08-1998
OXFORD UNIVERSITY PRESS
Subjects:
Agronomy. Soil science and plant productions
Aleurone cells
Aleurone layer
Apoptosis
Barley
Barley aleurone
Biological and medical sciences
Cell and Molecular Biology, Biochemistry and Molecular Physiology
Cell walls
DNA fragmentation
Economic plant physiology
Embryo development. Germination
Embryos
Fundamental and applied biological sciences. Psychology
Germination
Germination and dormancy
Grains
Growth and development
hydrolytic enzymes
Imbibition
Plant physiology and development
programmed cell death
Monocotyledones
Aleurone
Hordeum vulgare
Enzyme
Germination
Spatial variation
Time variation
Cereal crop
Cell wall
Fragmentation
Hydrolysis
Regulation(control)
Gramineae
Glycosidases
DNA
Angiospermae
Tagging
Hydrolases
Spermatophyta
α-Amylase
O-Glycosidases
Apoptosis
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Summary:During germination of barley grains, the appearance of DNA fragmentation started in aleurone cells near the embryo and extended to the distal end in a timedependent manner. DNA fragmentation was demonstrated to occur only after the expression of a-amylase mRNA in the aleurone layer. In addition, cell wall degradation started in cells near the embryo on the sides facing the endosperm. Subsequently cell wall degradation extended to the lateral cell walls and to cells more to the distal end of the grain. A typical alteration of the nucleus was observed by electron microscopy and an almost complete degradation of DNA was found in the nucleus while the nuclear envelope remained intact. The results indicate that programmed cell death occurred in aleurone cells during germination. A model is proposed for the regulation of programmed cell death in aleurone cells during germination involving ABA levels and cell wall degradation.
Bibliography:istex:607C1AEE58D731130CE64313837EB697AFF49EFD
ark:/67375/HXZ-37ZN8ZBF-S
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/49.325.1293