Impairment of novel non-coding small RNA00203 inhibits biofilm formation and reduces biofilm-specific antibiotic resistance in Acinetobacter baumannii

Impairment of novel non-coding small RNA00203 inhibits biofilm formation and reduces biofilm-specific antibiotic resistance in Acinetobacter baumannii

•Impairment of sRNA00203 in a clinical Acinetobacter baumannii substantially inhibited biofilm formation.•Impairment of sRNA00203 sensitized biofilm cells to imipenem and ciprofloxacin.•Knockout of gene encoding sRNA00203 significantly downregulated the expression of genes encoding matrix synthesis...

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Bibliographic Details
Published in:International journal of antimicrobial agents Vol. 62; no. 3; p. 106889
Main Authors: Shenkutie, Abebe Mekuria, Gebrelibanos, Daniel, Yao, Mianzhi, Bedada Hundie, Gadissa, Chow, Franklin W.N., Leung, Polly H.M.
Format: Journal Article
Language:English
Published: Netherlands Elsevier Ltd 01-09-2023
Subjects:
Acinetobacter baumannii
Antibiotics
Biofilm
Gene expression
Non-coding RNA
Resistance
RNA sequencing
Small RNA
Virulence
Resistance
RNA sequencing
Acinetobacter baumannii
Antibiotics
Small RNA
Virulence
Biofilm
Gene expression
Non-coding RNA
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Summary:•Impairment of sRNA00203 in a clinical Acinetobacter baumannii substantially inhibited biofilm formation.•Impairment of sRNA00203 sensitized biofilm cells to imipenem and ciprofloxacin.•Knockout of gene encoding sRNA00203 significantly downregulated the expression of genes encoding matrix synthesis (pgaB), efflux pumps (novel00738), lipopolysaccharide biosynthesis (novel00626), preprotein translocase subunit (secA) and the CRP transcriptional regulator. Small RNAs (sRNAs) are post-transcriptional regulators of many biological processes in bacteria, including biofilm formation and antibiotic resistance. The mechanisms by which sRNA regulates the biofilm-specific antibiotic resistance in Acinetobacter baumannii have not been reported to date. This study aimed to investigate the influence of sRNA00203 (53 nucleotides) on biofilm formation, antibiotic susceptibility, and expression of genes associated with biofilm formation and antibiotic resistance. The results showed that deletion of the sRNA00203-encoding gene decreased the biomass of biofilm by 85%. Deletion of the sRNA00203-encoding gene also reduced the minimum biofilm inhibitory concentrations for imipenem and ciprofloxacin 1024- and 128-fold, respectively. Knocking out of sRNA00203 significantly downregulated genes involved in biofilm matrix synthesis (pgaB), efflux pump production (novel00738), lipopolysaccharide biosynthesis (novel00626), preprotein translocase subunit (secA) and the CRP transcriptional regulator. Overall, the suppression of sRNA00203 in an A. baumannii ST1894 strain impaired biofilm formation and sensitized the biofilm cells to imipenem and ciprofloxacin. As sRNA00203 was found to be conserved in A. baumannii, a therapeutic strategy targeting sRNA00203 may be a potential solution for the treatment of biofilm-associated infections caused by A. baumannii. To the best of the authors’ knowledge, this is the first study to show the impact of sRNA00203 on biofilm formation and biofilm-specific antibiotic resistance in A. baumannii.
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ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2023.106889