Search Results - "Gebler, John"

Orthogonality of Separation in Two-Dimensional Liquid Chromatography by Gilar, Martin, Olivova, Petra, Daly, Amy E, Gebler, John C

“…Two-dimensional liquid chromatography is often used to reduce the proteomic sample complexity prior to tandem mass spectrometry analysis. The 2D-LC performance…”
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Analysis of Oligosaccharides Derived from Heparin by Ion-Pair Reversed-Phase Chromatography/Mass Spectrometry by Doneanu, Catalin E, Chen, Weibin, Gebler, John C

“…Current chromatographic and mass spectrometric techniques have limitations for analyzing heparin and heparin oligomers due to their high polarity, structural…”
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Two-dimensional separation of peptides using RP-RP-HPLC system with different pH in first and second separation dimensions by Gilar, Martin, Olivova, Petra, Daly, Amy E., Gebler, John C.

“…Two‐dimensional high performance liquid chromatography is a useful tool for proteome analysis, providing a greater peak capacity than single‐dimensional LC…”
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Reversed-phase ion-pair liquid chromatography analysis and purification of small interfering RNA by McCarthy, Sean M., Gilar, Martin, Gebler, John

“…Small interfering RNA (siRNA)-induced gene silencing shows great promise in genomic research and therapeutic applications. siRNA duplexes are typically…”
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Glycan characterization of the NIST RM monoclonal antibody using a total analytical solution: From sample preparation to data analysis by Hilliard, Mark, Alley, Jr, William R, McManus, Ciara A, Yu, Ying Qing, Hallinan, Sinead, Gebler, John, Rudd, Pauline M

“…Glycosylation is an important attribute of biopharmaceutical products to monitor from development through production. However, glycosylation analysis has…”
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Electrospray Ionization Quadrupole Ion-Mobility Time-of-Flight Mass Spectrometry as a Tool to Distinguish the Lot-to-Lot Heterogeneity in N-Glycosylation Profile of the Therapeutic Monoclonal Antibody Trastuzumab by Damen, Carola W.N., Chen, Weibin, Chakraborty, Asish B., van Oosterhout, Mike, Mazzeo, Jeffrey R., Gebler, John C., Schellens, Jan H.M., Rosing, Hilde, Beijnen, Jos H.

“…Monoclonal antibodies are typically glycosylated at asparagine residues in the Fc domain, and glycosylation heterogeneity at the Fc sites is well known. This…”
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Enzyme-Friendly, Mass Spectrometry-Compatible Surfactant for In-Solution Enzymatic Digestion of Proteins by Yu, Ying-Qing, Gilar, Martin, Lee, Peter J, Bouvier, Edouard S. P, Gebler, John C

“…Improved in-solution tryptic digestion of proteins in terms of speed and peptide coverage was achieved with the aid of a novel acid-labile anionic surfactant…”
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Determination of N-glycosylation sites and site heterogeneity in a monoclonal antibody by electrospray quadrupole ion-mobility time-of-flight mass spectrometry by Olivova, Petra, Chen, Weibin, Chakraborty, Asish B., Gebler, John C.

“…This paper presents an improved analytical method for glycosylation structural characterizations of a monoclonal antibody (mAb) using a newly developed…”
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Characterization of Protein Impurities and Site-Specific Modifications Using Peptide Mapping with Liquid Chromatography and Data Independent Acquisition Mass Spectrometry by Xie, Hongwei, Gilar, Martin, Gebler, John C

“…Liquid chromatography (LC)-based peptide mapping is extensively used for establishing protein identity, assessing purity, and detecting post-translational…”
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Mixed-mode chromatography for fractionation of peptides, phosphopeptides, and sialylated glycopeptides by Gilar, Martin, Yu, Ying-Qing, Ahn, Joomi, Fournier, Jennifer, Gebler, John C.

“…A mixed-mode chromatographic (MMC) sorbent was prepared by functionalizing the silica sorbent with a pentafluorophenyl (PFP) ligand. The resulting stationary…”
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Implications of column peak capacity on the separation of complex peptide mixtures in single- and two-dimensional high-performance liquid chromatography by Gilar, Martin, Daly, Amy E., Kele, Marianna, Neue, Uwe D., Gebler, John C.

“…Column peak capacity was utilized as a measure of column efficiency for gradient elution conditions. Peak capacity was evaluated experimentally for…”
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Use of an integrated MS – multiplexed MS/MS data acquisition strategy for high‐coverage peptide mapping studies by Chakraborty, Asish B., Berger, Scott J., Gebler, John C.

“…Liquid chromatography/mass spectrometry (LC/MS) peptide maps have become a basic tool for characterizing proteins of biological and pharmaceutical interest…”
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Comparison of 1-D and 2-D LC MS/MS methods for proteomic analysis of human serum by Gilar, Martin, Olivova, Petra, Chakraborty, Asish B., Jaworski, Aleksander, Geromanos, Scott J., Gebler, John C.

“…1‐D and 2‐D LC methods were utilized for proteome analysis of undepleted human serum. Separation of peptides in 2‐D LC was performed either with strong cation…”
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Identification of N-Linked Glycosylation Sites Using Glycoprotein Digestion with Pronase Prior to MALDI Tandem Time-of-Flight Mass Spectrometry by Yu, Ying Qing, Fournier, Jennifer, Gilar, Martin, Gebler, John C

“…Glycopeptides are typically prepared by cleaving the proteins with specific proteolytic enzymes, such as trypsin. The resulting glycopeptides tend to have weak…”
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Improving de Novo Sequencing of Peptides Using a Charged Tag and C-Terminal Digestion by Chen, Weibin, Lee, Peter J, Shion, Henry, Ellor, Nicholas, Gebler, John C

“…An improved method for peptide de novo sequencing by MALDI mass spectrometry is presented. The method couples a charge derivatization reaction with C-terminal…”
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A rapid sample preparation method for mass spectrometric characterization of N-linked glycans by Yu, Ying Qing, Gilar, Martin, Kaska, Jennifer, Gebler, John C.

“…A rapid method for analysis of glycans of glycoproteins is presented. This method comprised deglycosylation, sample cleanup and matrix‐assisted laser…”
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Effects of column length, particle size, gradient length and flow rate on peak capacity of nano-scale liquid chromatography for peptide separations by Liu, Hongji, Finch, Jeffrey W., Lavallee, Michael J., Collamati, Robert A., Benevides, Christopher C., Gebler, John C.

“…The effects of the column length, the particle size, the gradient length and the flow rate of a nanoLC system on peptide peak capacity were investigated and…”
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Peptide retention prediction applied to proteomic data analysis by Gilar, Martin, Jaworski, Aleksander, Olivova, Petra, Gebler, John C.

“…A retention prediction model was developed for peptides separated in reversed‐phase chromatography. The model was utilized to identify and exclude the false…”
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Analysis of native and chemically modified oligonucleotides by tandem ion-pair reversed-phase high-performance liquid chromatography/electrospray ionization mass spectrometry by Fountain, Kenneth J., Gilar, Martin, Gebler, John C.

“…Ion‐pair reversed‐phase high‐performance liquid chromatography (IP‐RP‐HPLC) was utilized in tandem with negative‐ion electrospray ionization time‐of‐flight…”
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Ion-pair reversed-phase high-performance liquid chromatography analysis of oligonucleotides:: Retention prediction by Gilar, Martin, Fountain, Kenneth J., Budman, Yeva, Neue, Uwe D., Yardley, Kurt R., Rainville, Paul D., Russell II, Reb J., Gebler, John C.

“…An ion-pair reversed-phase HPLC method was evaluated for the separation of synthetic oligonucleotides. Mass transfer in the stationary phase was found to be a…”
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