Biofilm-overproducing Bacillus subtilis B12ΔYwcc decreases Cd uptake in Chinese cabbage through increasing Cd-immobilizing related gene abundance and root surface colonization

•One biofilm-overproducing mutant Bacillus subtilis strain B12 were constructed.•B12 induced lower Cd availability and uptake in edible tissue of Chinese cabbage.•B12 induced higher polysaccharide content and invertase activity in soil.•B12 induced higher Cd-adsorption related cumA, epsA, and cadA g...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of environmental sciences (China) Vol. 120; pp. 84 - 93
Main Authors:	Ge, Yanyan, Ge, Zhanbiao, Zheng, Jinwei, Sheng, Xiafang, He, Linyan
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-10-2022
Subjects:	Bacillus subtilis - genetics Bacterial colonization and biofilm formation Biofilm-producing Bacillus subtilis Biofilms Brassica Cadmium - analysis Cadmium - toxicity Cd uptake in vegetable Cd- stabilizing related genes China Mutant strain with biofilm overproduction Polysaccharides Soil Soil Pollutants - analysis Vegetables China Cd uptake in vegetable Biofilm-producing Bacillus subtilis Bacterial colonization and biofilm formation Mutant strain with biofilm overproduction Cd- stabilizing related genes
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	•One biofilm-overproducing mutant Bacillus subtilis strain B12 were constructed.•B12 induced lower Cd availability and uptake in edible tissue of Chinese cabbage.•B12 induced higher polysaccharide content and invertase activity in soil.•B12 induced higher Cd-adsorption related cumA, epsA, and cadA gene copies in soil.•B12 showed higher colonization and biofilm formation on the vegetable root surface. Biofilm-producing bacteria can decrease Cd uptake in vegetables, but mechanisms underlying this effect are poorly characterized. In this study, two mutant strains B12ΔYwcc and B12ΔSlrR were constructed from a biofilm-producing Bacillus subtilis strain B12. Then, the impacts of strain B12 and its high biofilm-producing mutant strain B12ΔYwcc and low biofilm-producing mutant strain B12ΔSlrR on Cd availability and uptake in Chinese cabbage and the related mechanisms were investigated in the Cd-polluted soil. Strain B12 and its mutants B12ΔYwcc and B12ΔSlrR increased the dry biomasses of edible tissues by 54%–130% compared with the controls. Strain B12 and its mutant B12ΔYwcc reduced the soil available Cd content by 36%–50% and root and edible tissue Cd contents by 23%–50% compared with the controls. Furthermore, the mutant strain B12ΔYwcc reduced the edible tissue Cd content by 40% and increased the polysaccharide content by 23%, invertase activity by 139%, and gene copies of the cumA by 4.5-fold, epsA by 7.1-fold, and cadA by 4.3-fold, which were involved in Cd adsorption in the rhizosphere soils, respectively, compared with strain B12. The polysaccharide content and cumA, epsA, and cadA gene copy numbers showed significantly reverse correlations with the available Cd content. Notably, the mutant strain B12ΔYwcc showed better ability to colonize the vegetable root surface than strain B12. These findings demonstrated that the biofilm-overproducing mutant strain B12ΔYwcc increased the polysaccharide production and Cd-immobilizing related cumA, epsA, and cadA gene copies, resulting in lower Cd availability and accumulation in Chinese cabbage in the Cd-polluted soil.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1001-0742 1878-7320
DOI:	10.1016/j.jes.2022.01.016