Search Results - "Gazaryan, I."

The molecular chaperone sigma 1 receptor mediates rescue of retinal cone photoreceptor cells via modulation of NRF2 by Wang, J., Zhao, J., Cui, X., Mysona, B.A., Navneet, S., Saul, A., Ahuja, M., Lambert, N., Gazaryan, I.G., Thomas, B., Bollinger, K.E., Smith, S.B.

“…Sigma 1 receptor (Sig1R), a putative molecular chaperone, has emerged as a novel therapeutic target for retinal degenerative disease. Earlier studies showed…”
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Zinc Switch in Pig Heart Lipoamide Dehydrogenase: Steady-State and Transient Kinetic Studies of the Diaphorase Reaction by Gazaryan, I. G., Shchedrina, V. A., Klyachko, N. L., Zakhariants, A. A., Kazakov, S. V., Brown, A. M.

“…Elevation of intracellular Zn 2+ following ischemia contributes to cell death by affecting mitochondrial function. Zn 2+ is a differential regulator of the…”
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Direct electron transfer between heme-containing enzymes and electrodes as basis for third generation biosensors by Gorton, L., Lindgren, A., Larsson, T., Munteanu, F.D., Ruzgas, T., Gazaryan, I.

“…Direct electron transfer (DET) between redox enzymes and electrodes found the basis for third generation biosensors. Recent investigations in the authors’…”
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High Throughput Screening in Drug Discovery: Problems and Solutions by Hushpulian, D. M., Gaisina, I. N., Nikulin, S. V., Chubar, T. A., Savin, S. S., Gazaryan, I. G., Tishkov, V. I.

“…World-wide introduction of high throughput screening (HTS) methods in drug discovery research did not result in the increased number of novel medications on…”
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Structure-activity relationship for branched oxyquinoline HIF activators: Effect of modifications to phenylacetamide “tail” by Poloznikov, A.A., Zakhariants, A.A., Nikulin, S.V., Smirnova, N.A., Hushpulian, D.M., Gaisina, I.N., Tonevitsky, A.G., Tishkov, V.I., Gazaryan, I.G.

“…HIF prolyl hydroxylase is a major regulator of HIF stability. Branched tail oxyquinolines have been identified as specific inhibitors of HIF prolyl hydroxylase…”
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Antihypoxic activity of adaptaquin enantiomers by Gaisina, I. N., Khristichenko, A. Yu, Gaisin, A. M., Smirnova, N. A., Gazaryan, I. G., Poloznikov, A. A.

“…Adaptaquin, 7-{(4-chlorophenyl)[(3-hydroxypyridin-2-yl)amino]methyl}quinolin-8-ol ( 1 ), was identified in our earlier study as a hypoxia-inducible factor…”
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Fast Responding Genes to HIF Prolyl Hydroxylase Inhibitors by Hushpulian, D. M., Nikulin, S. V., Chubar, T. A., Khristichenko, A. Yu, Poloznikov, A. A., Gazaryan, I. G.

“…The attempts to elucidate the mechanism of the hypoxic activation of erythropoietin synthesis lead to the discovery of the hypoxia inducible factor (HIF) and…”
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Comparison of physico-chemical characteristics of four laccases from different basidiomycetes by Shleev, S.V., Morozova, O.V., Nikitina, O.V., Gorshina, E.S., Rusinova, T.V., Serezhenkov, V.A., Burbaev, D.S., Gazaryan, I.G., Yaropolov, A.I.

“…New strains of basidiomycetes producing extracellular laccases ( Trametes ochracea 92-78, and Trametes hirsuta 56) have been found by screening of isolates of…”
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Enzyme–substrate reporters for evaluation of substrate specificity of HIF prolyl hydroxylase isoforms by Osipyants, A. I., Smirnova, N. A., Khristichenko, A. Yu, Hushpulian, D. M., Nikulin, S. V., Chubar, T. A., Zakhariants, A. A., Tishkov, V. I., Gazaryan, I. G., Poloznikov, A. A.

“…An organism naturally responds to hypoxia via stabilization of hypoxia-inducible factor (HIF). There are three isoforms of HIFα subunits whose stability is…”
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Structural optimization of adaptaquin, a HIF prolyl hydroxylase inhibitor by Poloznikov, A. A., Khristichenko, A. Yu, Smirnova, N. A., Hushpulian, D. M., Gaisina, I. N., Osipyants, A. I., Tishkov, V. I., Gazaryan, I. G.

“…The structural optimization of adaptaquin, viz. , 7-(4-chlorophenyl)[(3-hydroxypyridin-2-yl)amino]methylquinolin-8-ol, a HIF (hypoxia inducible factor) prolyl…”
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High-yield reactivation of anionic tobacco peroxidase overexpressed in Escherichia coli by Zakharova, G.S., Poloznikov, A.A., Chubar, T.A., Gazaryan, I.G., Tishkov, V.I.

“…•Refolding conditions for recombinant tobacco peroxidase (TOP) were optimized.•Reactivation yield for TOP was increased up to 85% of solubilized…”
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HIF2 ODD-luciferase reporter: the most sensitive assay for HIF prolyl hydroxylase inhibitors by Smirnova, N. A., Osipyants, A. I., Khristichenko, A. Yu, Hushpulian, D. M., Nikulin, S. V., Chubar, T. A., Zakhariants, A. A., Tishkov, V. I., Gazaryan, I. G., Poloznikov, A. A.

“…Reporters expressing fusion proteins of HIF2 and HIF3 C-terminal oxygen degradable domain (ODD) with the firefly luciferase, HIF2 ODD-luc and HIF3 ODD-luc,…”
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Site-directed mutagenesis of tobacco anionic peroxidase: Effect of additional aromatic amino acids on stability and activity by Poloznikov, A.A., Zakharova, G.S., Chubar, T.A., Hushpulian, D.M., Tishkov, V.I., Gazaryan, I.G.

“…Tobacco anionic peroxidase (TOP) is known to effectively catalyze luminol oxidation without enhancers, in contrast to horseradish peroxidase (HRP). To pursue…”
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Optimization of Neh2-Luc Reporter for Screening of Activators of Antioxidant Program by Khristichenko, Yu, Hushpulian, D. M., Smirnova, N. A., Zakhariants, A. A., Chubar, T. A., Tishkov, V. I., Gazaryan, I. G., Poloznikov, A. A.

“…— Reporter vectors expressing of amino acid residues 16–96 and 16–85 from Neh2-domain of Nrf2 transcription factor fused to firefly luciferase have been…”
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Quantitative Analysis of Cell-Based Luciferase Fusion Reporters by Khristichenko, A. Yu, Poloznikov, A. A., Hushpulian, D. M., Smirnova, N. A., Zakhariants, A. A., Kazakov, S. V., Tishkov, V.I., Gazaryan, I. G.

“…Cell-based reporters expressing luciferase fusions with transcription factors or their protein stability domains can be considered as microbioreactors…”
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Catalytic mechanism and substrate specificity of HIF prolyl hydroxylases by Smirnova, N. A., Hushpulian, D. M., Speer, R. E., Gaisina, I. N., Ratan, R. R., Gazaryan, I. G.

“…This review describes the catalytic mechanism, substrate specificity, and structural peculiarities of alpha-ketoglutarate dependent nonheme iron dioxygenases…”
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Antioxidant and antihypoxic properties of neuroprotective drugs by Poloznikov, A. A., Smirnova, N. A., Khristichenko, A. Yu, Hushpulian, D. M., Nikulin, S. V., Tishkov, V. I., Gaisina, I. N., Gazaryan, I. G.

“…The screening of the library containing 320 drugs and biologically active compounds was carried out. The library was created for testing potent agents on Rett…”
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Metal Ions as Activators of Hypoxia Inducible Factor by Osipyants, A. I., Smirnova, N. A., Khristichenko, A. Yu, Nikulin, S. V., Zakhariants, A. A., Tishkov, V. I., Gazaryan, I. G., Poloznikov, A. A.

“…Activation of antihypoxic program under the action of a number of transition and heavy metals has been studied using cell-based HIF1 ODD-luc and HRE-luc…”
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Electrochemistry and kinetics of fungal laccase mediators by Shumakovich, G.P., Shleev, S.V., Morozova, O.V., Khohlov, P.S., Gazaryan, I.G., Yaropolov, A.I.

“…The screening of potential redox mediators for laccase was performed using homogeneous Trametes hirsuta laccase. Heterogeneous (electrochemical) and…”
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Purification and unusual kinetic properties of a tobacco anionic peroxidase by Gazaryan, Irina G, Lagrimini, L.Mark

“…The tobacco anionic peroxidase has been isolated from the leaves of transgenic Nicotiana sylvestris plants overproducing this enzyme. The plant expression…”
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