Search Results - "Gaza‐Bulseco, Georgeen"

“High‐risk” host cell proteins (HCPs): A multi‐company collaborative view by Jones, Marisa, Palackal, Nisha, Wang, Fengqiang, Gaza‐Bulseco, Georgeen, Hurkmans, Karen, Zhao, Yiwei, Chitikila, Carmelata, Clavier, Severine, Liu, Suli, Menesale, Emily, Schonenbach, Nicole S., Sharma, Satish, Valax, Pascal, Waerner, Thomas, Zhang, Lei, Connolly, Trish

“…Host cell proteins (HCPs) are process‐related impurities that may copurify with biopharmaceutical drug products. Within this class of impurities there are some…”
Get full text

Comparison of methionine oxidation in thermal stability and chemically stressed samples of a fully human monoclonal antibody by Chumsae, Chris, Gaza-Bulseco, Georgeen, Sun, Joanne, Liu, Hongcheng

“…Methionine (Met) oxidation is a major degradation pathway of protein therapeutics. Met oxidation of a fully human recombinant monoclonal antibody was…”
Get full text

Effect of methionine oxidation of a recombinant monoclonal antibody on the binding affinity to protein A and protein G by Gaza-Bulseco, Georgeen, Faldu, Sagar, Hurkmans, Karen, Chumsae, Chris, Liu, Hongcheng

“…Oxidation of methionine (Met) residues is one of the most common protein degradation pathways. Two Met residues, Met256 and Met432, of a recombinant fully…”
Get full text

Characterization of the stability of a fully human monoclonal IgG after prolonged incubation at elevated temperature by Liu, Hongcheng, Gaza-Bulseco, Georgeen, Sun, Joanne

“…The susceptible degradation sites of therapeutic proteins are routinely assessed under accelerated conditions such as exposure to chemicals or incubation at…”
Get full text

Assessment of antibody fragmentation by reversed-phase liquid chromatography and mass spectrometry by Liu, Hongcheng, Gaza-Bulseco, Georgeen, Lundell, Edwin

“…Antibody fragmentation in the hinge region and other regions, and the impact of pH on the level and pattern of antibody fragmentation were investigated by…”
Get full text

Effect of posttranslational modifications on the thermal stability of a recombinant monoclonal antibody by Liu, Hongcheng, Bulseco, Georgeen-Gaza, Sun, Joanne

“…The effect of oligosaccharides and C-terminal lysine residues on the thermal stability of a recombinant IgG 1 monoclonal antibody was investigated using…”
Get full text

Heterogeneity of monoclonal antibodies by Liu, Hongcheng, Gaza-Bulseco, Georgeen, Faldu, Dinesh, Chumsae, Chris, Sun, Joanne

“…Heterogeneity of monoclonal antibodies is common due to the various modifications introduced over the lifespan of the molecules from the point of synthesis to…”
Get more information

Fragmentation of a Recombinant Monoclonal Antibody at Various pH by Gaza-Bulseco, Georgeen, Liu, Hongcheng

“…Purpose To determine the relative importance of direct hydrolysis and β-elimination, two common mechanisms of antibody hinge region fragmentation, and the…”
Get full text

Ranking the Susceptibility of Disulfide Bonds in Human IgG1 Antibodies by Reduction, Differential Alkylation, and LC−MS Analysis by Liu, Hongcheng, Chumsae, Chris, Gaza-Bulseco, Georgeen, Hurkmans, Karen, Radziejewski, Czeslaw H

“…One of the basic structural features of human IgG1 is the arrangement of the disulfide bond structure, 4 inter chain disulfide bonds in the hinge region and 12…”
Get full text

Glutamine deamidation of a recombinant monoclonal antibody by Liu, Hongcheng, Gaza-Bulseco, Georgeen, Chumsae, Chris

“…Deamidation of glutamine (Gln) proceeds at a much slower rate than deamidation of asparagine (Asn) residues at peptide level. However, deamidation of Gln…”
Get full text

Characterization of lower molecular weight artifact bands of recombinant monoclonal IgG1 antibodies on non-reducing SDS-PAGE by Liu, Hongcheng, Gaza-Bulseco, Georgeen, Chumsae, Chris, Newby-Kew, Abigail

“…SDS-PAGE under non-reducing conditions is one of the most commonly used techniques for recombinant monoclonal antibody purity and stability indicating assay…”
Get full text

Mass Spectrometry Analysis of Photo-Induced Methionine Oxidation of a Recombinant Human Monoclonal Antibody by Liu, Hongcheng, Gaza-Bulseco, Georgeen, Zhou, Lisa

“…Oxidation of methionine (Met) residues of a recombinant fully human monoclonal antibody after exposure to light was investigated and compared with chemically…”
Get full text

Characterization of the glycosylation state of a recombinant monoclonal antibody using weak cation exchange chromatography and mass spectrometry by Gaza-Bulseco, Georgeen, Bulseco, Ashley, Chumsae, Chris, Liu, Hongcheng

“…Recombinant monoclonal antibody heterogeneity is inherent due to various enzymatic and non-enzymatic modifications. In this study, a recombinant humanized…”
Get full text

Mass spectrometry analysis of in vitro nitration of a recombinant human IgG1 monoclonal antibody by Liu, Hongcheng, Gaza-Bulseco, Georgeen, Chumsae, Chris, Radziejewski, Czeslaw H.

“…Nitration of a recombinant human monoclonal antibody was carried out in vitro by incubating the antibody with the nitrating reagent tetranitromethane (TNM)…”
Get full text

Analysis of Reduced Monoclonal Antibodies Using Size Exclusion Chromatography Coupled with Mass Spectrometry by Liu, Hongcheng, Gaza-Bulseco, Georgeen, Chumsae, Chris

“…Size-exclusion chromatography (SEC) has been widely used to detect antibody aggregates, monomer, and fragments. SEC coupled to mass spectrometry has been…”
Get full text

A 13C NMR approach to categorizing potential limitations of α,β-unsaturated carbonyl systems in drug-like molecules by Cusack, Kevin P., Arnold, Lee D., Barberis, Claude E., Chen, Haipeng, Ericsson, Anna M., Gaza-Bulseco, Georgeen S., Gordon, Thomas D., Grinnell, Christine M., Harsch, Andreas, Pellegrini, Maria, Tarcsa, Edit

“…Compounds that contain an α,β-unsaturated carbonyl moiety are often flagged as potential Michael acceptors. All α,β-unsaturated carbonyl moieties are not…”
Get full text

Identification and Localization of Unpaired Cysteine Residues in Monoclonal Antibodies by Fluorescence Labeling and Mass Spectrometry by Chumsae, Chris, Gaza-Bulseco, Georgeen, Liu, Hongcheng

“…Each human IgG1 antibody contains a total of thirty-two cysteine residues. In theory, all of them are involved in disulfide bonds, and no free sulfhydryl…”
Get full text

Structural effect of deglycosylation and methionine oxidation on a recombinant monoclonal antibody by Liu, Hongcheng, Gaza-Bulseco, Georgeen, Xiang, Tao, Chumsae, Chris

“…Methionine (Met) is one of the most susceptible amino acids to oxidation. Met256 (CH2-Met15.1) and Met432 (CH3-Met107) of a recombinant humanized monoclonal…”
Get full text

Method to Differentiate Asn Deamidation That Occurred Prior to and during Sample Preparation of a Monoclonal Antibody by Gaza-Bulseco, Georgeen, Li, Biqin, Bulseco, Ashley, Liu, Hongcheng

“…Asparagine (Asn) deamidation is a major source of antibody instability and micro heterogeneity. For this reason, it is critical to accurately characterize both…”
Get full text

Domain-level stability of an antibody monitored by reduction, differential alkylation, and mass spectrometry analysis by Liu, Hongcheng, Chumsae, Chris, Gaza-Bulseco, Georgeen, Goedken, Eric R.

“…Human immunoglobulin G1 (IgG1) contains 12 domains, and each has an intrachain disulfide bond that connects the two layers of antiparallel β-sheets. These…”
Get full text