Identification of a Highly Immunoreactive Epitope of Brugia malayi TPx Recognized by the Endemic Sera

Filarial thiordoxin peroxidase is a major antioxidant that plays a crucial role in parasite survival. Although Brugia malayi TPx has been shown to be a potential vaccine candidate, it shares 63% homology with its mammalian counterpart, limiting its use as a vaccine or drug target. In silico analysis...

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Bibliographic Details
Published in:	The Journal of parasitology Vol. 96; no. 6; pp. 1228 - 1229
Main Authors:	Madhumathi, Jayaprakasam, Prince, Prabhu Rajaiah, Gayatri, Subash Chellam, Aparnaa, Ramanathan, Kaliraj, Perumal
Format:	Journal Article
Language:	English
Published:	Lawrence, KS American Society of Parasitologists 01-12-2010 Allen Press Inc
Subjects:	Animals Antioxidants Biological and medical sciences Brugia malayi Brugia malayi - enzymology Brugia malayi - immunology Chronic infection Drug development Elephantiasis, Filarial - blood Elephantiasis, Filarial - immunology Elephantiasis, Filarial - parasitology Endemic Diseases Enzymes Epitopes Epitopes, B-Lymphocyte - immunology Filarial elephantiasis Filariasis Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Homology Human subjects Humans Immune Sera - immunology Immunosuppressive agents Infections Initiatives Invertebrates Nematodes Parasites Parasitology Peptides Peptides - chemistry Peptides - immunology Peroxidase Peroxiredoxins - chemistry Peroxiredoxins - immunology Peroxiredoxins - isolation & purification Proteins Public health Reactivity Recombinant Proteins - chemistry Recombinant Proteins - immunology Recombinant Proteins - isolation & purification RESEARCH NOTES Survival Thioredoxin Vaccination Vaccines Vaccines - immunology Tamil Nadu India India Helmintha Parasite Nemathelminthia Invertebrata Brugia malayi Nematoda
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Summary:	Filarial thiordoxin peroxidase is a major antioxidant that plays a crucial role in parasite survival. Although Brugia malayi TPx has been shown to be a potential vaccine candidate, it shares 63% homology with its mammalian counterpart, limiting its use as a vaccine or drug target. In silico analysis of TPx sequence revealed a linear B epitope in the host's nonhomologous region. The peptide sequence (TPx peptide27–48) was synthesized, and its reactivity with clinical sera from an endemic region was analyzed. The peptide showed significantly high reactivity (P < 0.05) against the sera of putatively immune individuals compared to the nonendemic control sera. It also showed high reactivity against the sera of patients with chronic pathology and patent infection. The high reactivity of the peptide with endemic immune sera equivalent to that of whole protein shows that it forms a dominant B epitope of TPx protein and thus could be utilized for incorporation into a multiepitope vaccine construct for filariasis.
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ISSN:	0022-3395 1937-2345
DOI:	10.1645/GE-2532.1