LC-MS Method Development for Simultaneous Determination of Trans-3′-hydroxycotinine and Three Mercapturic Acids in Urine

LC-MS Method Development for Simultaneous Determination of Trans-3′-hydroxycotinine and Three Mercapturic Acids in Urine

The negative impact of tobacco smoke on the human body is due to a wide range of harmful substances including volatile organic compounds (VOCs). Some VOCs of tobacco smoke metabolize in human organism into mercapturic acids (MAs). The determination of the amount of MAs in readily available biologica...

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Bibliographic Details
Published in:BioNanoScience Vol. 8; no. 3; pp. 924 - 929
Main Authors: Lopukhov, L. V., Laikov, A. V., Romanova, V. A., Gatina, D. Z., Lopukhov, V. L., Abdulkhakov, S. R., Zaitseva, T. A., Medvedeva, S. N., Gnuchikh, E. V., Salafutdinov, I. I., Grigoryeva, T. V.
Format: Journal Article
Language:English
Published: New York Springer US 01-09-2018
Springer Nature B.V
Subjects:
Acids
Biological and Medical Physics
Biomaterials
Biophysics
Circuits and Systems
Cysteine
Drug addiction
Engineering
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolites
Nanotechnology
Nicotine
Organic compounds
Smoke
Smoking
Smoking cessation
Statistical analysis
Tobacco
Tobacco smoke
Urine
VOCs
Volatile organic compounds
Tobacco
LC-MS
Mercapturic acids
Nicotine
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Summary:The negative impact of tobacco smoke on the human body is due to a wide range of harmful substances including volatile organic compounds (VOCs). Some VOCs of tobacco smoke metabolize in human organism into mercapturic acids (MAs). The determination of the amount of MAs in readily available biological fluids, for example in urine, allows to assess the level of exposure of these VOCs in a particular person. It is useful to assess the impact of individual VOCs on the body together with the assessment of the intake of nicotine. The intake of nicotine can be determined by the content of its metabolites in the urine, in particular by the content of trans-3′-hydroxycotinine (tH-Cot). A joint assessment of the concentrations of trans-3′-hydroxycotinine and MAs in urine allows obtaining selective information about effects of different VOCs and nicotine on the smoker’s body. We have developed a liquid chromatography–mass spectrometry (LC-MS) method for simultaneous quantifying of tH-Cot and three MAs: N-Acetyl-S-(3-hydroxypropyl)cysteine (HPMA), N-Acetyl-S-(3-hydroxypropyl-1-methyl)-L-cysteine (HMPMA), N-Acetyl-S-(2-cyanoethyl)-L-cysteine (CEMA). We used this method to quantify the levels of MAs and tH-Cot in the urine of a group of 15 smokers just before and 5 days after smoking cessation. For all studied compounds, we have found statistically significant changes in concentration on the fifth day of smoking cessation. The method developed can be used to jointly assess the levels of exposure to nicotine and VOCs in the study of various tobacco products.
ISSN:2191-1630
2191-1649
DOI:10.1007/s12668-018-0528-1