Search Results - "Garde, J. Julián"
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Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality
Published in International journal of molecular sciences (16-04-2020)“…Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in…”
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Freezing-Thawing Procedures Remodel the Proteome of Ram Sperm before and after In Vitro Capacitation
Published in International journal of molecular sciences (17-09-2019)“…Mammalian sperm must undergo a set of structural and functional changes collectively termed as capacitation to ensure a successful oocyte fertilization…”
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3
Cryopreservation of ram sperm alters the dynamic changes associated with in vitro capacitation
Published in Theriogenology (15-03-2020)“…The aim of this study was to investigate the dynamic changes that ram sperm experience during in vitro capacitation before and after cryopreservation. Using…”
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4
Recombinant SPINK3 improves ram sperm quality and in vitro fertility after cryopreservation
Published in Theriogenology (01-03-2020)“…Capacitation-like changes affect sperm of several species, such as ram, reducing cell survival and fertilizing competence. Proteins from seminal plasma…”
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5
Increased chromatin fragmentation and reduced acrosome integrity in spermatozoa of red deer from lead polluted sites
Published in The Science of the total environment (01-02-2015)“…Vertebrates are constantly exposed to a diffuse pollution of heavy metals existing in the environment, but in some cases, the proximity to emission sources…”
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The Carbon Dioxide-Rumen Fermentation Processes-strategy, a proposal to sustain environmentally friendly dairy farms
Published in Journal of cleaner production (10-12-2018)“…A novel Carbon Dioxide Utilization (CDU) approach from a relatively minor biogenic carbon dioxide emission source, Rumen Fermentation Processes (RFP), is…”
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7
Reactive oxygen species generators affect quality parameters and apoptosis markers differently in red deer spermatozoa
Published in Reproduction (Cambridge, England) (01-02-2009)“…Fe2+/ascorbate, hydrogen peroxide (H2O2), and hypoxanthine/xanthine oxidase (XOD) are commonly used for inducing oxidative stress on spermatozoa. A comparative…”
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Sperm cell population dynamics in ram semen during the cryopreservation process
Published in PloS one (27-03-2013)“…Sperm cryopreservation has become an indispensable tool in biology. Initially, studies were aimed towards the development of efficient freezing protocols in…”
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Effects of vitrification on ram spermatozoa using free-egg yolk extenders
Published in Cryobiology (01-08-2015)“…The present study aimed to examine the behavior of ram spermatozoa subjected to a vitrification process in free-egg yolk diluents in relation with conventional…”
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10
Reduced glutathione and Trolox (vitamin E) as extender supplements in cryopreservation of red deer epididymal spermatozoa
Published in Animal reproduction science (01-11-2012)“…The use of assisted reproductive techniques in cervids is increasing as the commercial use of these species increase. We have tested the suitability of the…”
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Male Fertility in Natural Populations of Red Deer Is Determined by Sperm Velocity and the Proportion of Normal Spermatozoa
Published in Biology of reproduction (01-04-2005)“…Male reproductive success is determined by the ability of males to gain sexual access to females and by their ability to fertilize ova. Among polygynous…”
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12
Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments
Published in Reproduction fertility and development (2010)“…Antioxidants could improve sperm media, extending the viability of spermatozoa and protecting their DNA. The protective ability of lipoic acid, melatonin,…”
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13
Dynamics of sperm subpopulations based on motility and plasma membrane status in thawed ram spermatozoa incubated under conditions that support in vitro capacitation and fertilisation
Published in Reproduction fertility and development (01-01-2014)“…The present study evaluated modifications occurring in thawed ram spermatozoa during incubation in different media that supported in vitro capacitation and…”
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14
Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams
Published in PloS one (21-01-2014)“…The present study addresses the effect of heat stress on males' reproduction ability. For that, we have evaluated the sperm DNA fragmentation (DFI) by SCSA of…”
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15
Free-radical production after post-thaw incubation of ram spermatozoa is related to decreased in vivo fertility
Published in Reproduction fertility and development (01-01-2015)“…The aim of the present study was to evaluate the effect of sperm reactive oxygen species (ROS) production and DNA changes on male fertility. For that purpose,…”
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16
Identification of Optimal Concentrations and Incubation Times for the Study of In Vitro Effects of Pb in Ram Spermatozoa
Published in Bulletin of environmental contamination and toxicology (01-08-2013)“…In vitro effects of lead (Pb) on ram ( Ovis aries ) spermatozoa were studied to establish a threshold level that affects sperm function. Spermatozoa were…”
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Reproductive traits in captive and free-ranging males of the critically endangered Iberian lynx (Lynx pardinus)
Published in Reproduction (Cambridge, England) (01-01-2010)“…The Iberian lynx (Lynx pardinus) is the most endangered felid in the world. Adequate genetic management of in situ and ex situ populations, and linkage between…”
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18
Taking advantage of the use of supervised learning methods for characterization of sperm population structure related with freezability in the Iberian red deer
Published in Theriogenology (01-05-2012)“…Using Iberian red deer as a model, this study presents a supervised learning method, the Support Vector Machines (SVM), to characterize sperm population…”
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Assessment of semen quality, sperm cryopreservation and heterologous IVF in the critically endangered Iberian lynx (Lynx pardinus)
Published in Reproduction fertility and development (01-01-2009)“…Semen traits and factors affecting sperm cryopreservation were assessed in the Iberian lynx (Lynx pardinus), a species regarded as the most endangered felid in…”
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20
Seminal plasma improves cryopreservation of Iberian red deer epididymal sperm
Published in Theriogenology (01-11-2006)“…We tested the protective action of seminal plasma on epididymal spermatozoa from Iberian red deer, especially considering cryopreservation, as a means for…”
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