Bisphenol A and 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin at non‐cytotoxic doses alter the differentiation potential and cell function of rat adipose‐stem cells

Bisphenol A and 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin at non‐cytotoxic doses alter the differentiation potential and cell function of rat adipose‐stem cells

The possibility of chemical contamination is an important issue to consider when designing a cell therapy strategy. Both bisphenol A (BPA) and 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) are among the most environmentally relevant endocrine disrupting chemicals (EDCs, compounds with a high affinity f...

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Published in:Environmental toxicology Vol. 37; no. 9; pp. 2314 - 2323
Main Authors: Nunes, Helga Caputo, Tavares, Samara Costa, Garcia, Heloísa Vicente, Cucielo, Maira Smaniotto, Santos, Sérgio Alexandre Alcântara, Aal, Mirian Carolini Esgoti, Golim, Marjorie Assis, Justulin, Luís Antônio, Ribeiro, Amanda Oliveira, Deffune, Elenice, Scarano, Wellerson Rodrigo, Delella, Flávia Karina
Format: Journal Article
Language:English
Published: Hoboken, USA John Wiley & Sons, Inc 01-09-2022
Wiley Subscription Services, Inc
Subjects:
Adipocytes
Adipose tissue
Apoptosis
Biomedical materials
Bisphenol A
Body fat
Body organs
BPA
Cell differentiation
Cell therapy
Cell viability
Chemical contamination
Chemical pollution
Clinical trials
Contaminants
Contamination
Cytotoxicity
Damage
Deoxyribonucleic acid
Differentiation (biology)
Dioxins
DNA
DNA damage
Endocrine disruptors
Mesenchyme
rASCs
Regenerative medicine
Stem cell transplantation
Stem cells
Stromal cells
TCDD
Tissue
Tissue engineering
TCDD
rASCs
BPA
DNA damage
regenerative medicine
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Summary:The possibility of chemical contamination is an important issue to consider when designing a cell therapy strategy. Both bisphenol A (BPA) and 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) are among the most environmentally relevant endocrine disrupting chemicals (EDCs, compounds with a high affinity for adipose tissue) recently studied. Adipose‐derived stem cells (ASCs) are mesenchymal stromal cells (MSCs) obtained from adipose tissue widely used in regenerative medicine to prevent and treat diseases in several tissues and organs. Although the experimental use of tissue‐engineered constructs requires careful analysis for approval and implantation, there has been a recent increase in the number of approved clinical trials for this promising strategy. This study aimed to evaluate cell viability, apoptosis, DNA damage, and the adipogenic or osteogenic differentiation potential of rat adipose‐derived stem cells (rASCs) exposed to previously established non‐cytotoxic doses of BPA and TCDD in vitro. Results demonstrated that 10 μM of BPA and 10 nM of TCDD were able to significantly reduce cell viability, while all exposure levels resulted in DNA damage, although did not increase the apoptosis rate. According to the analysis of adipogenic differentiation, 1 μM of BPA induced the significant formation of oil droplets, suggesting an increased adipocyte differentiation, while both 10 μM of BPA and 10 nM of TCDD decreased adipocyte differentiation. Osteogenic differentiation did not differ among the treatments. As such, BPA and TCDD in the concentrations tested can modify important processes in rASCs such as cell viability, adipogenic differentiation, and DNA damage. Together, these findings prove that EDCs play an important role as contaminants, putatively interfering in cell differentiation and thus impairing the therapeutic use of ASCs.
Bibliography:Funding information
Conselho Nacional de Desenvolvimento Científico e Tecnológico
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1520-4081
1522-7278
DOI:10.1002/tox.23598