High Rates of New Delhi Metallo-β-Lactamase Carbapenemase Genes in Multi-Drug Resistant Gram-Negative Bacteria in Nicaragua

Antimicrobial resistance (AMR) is a global public health crisis. Much of the burden of AMR in resource-limited settings remains unknown. This pilot study characterized clinical isolates of multidrug-resistant Gram-negative rods (MDR-GNRs) from Nicaragua. New Delhi metallo-β-lactamase (NDM) carbapene...

Full description

Saved in:

Bibliographic Details
Published in:	The American journal of tropical medicine and hygiene Vol. 102; no. 2; pp. 384 - 387
Main Authors:	Dretler, Alexandra W, Avila, Julissa, Sandoval Lira, Lissette, García Rener, Marcia, Burger-Calderon, Raquel, Hargita, Michelle N, Page, Alexander M, Waggoner, Jesse J, Satola, Sarah W
Format:	Journal Article
Language:	English
Published:	United States Institute of Tropical Medicine 01-02-2020 The American Society of Tropical Medicine and Hygiene
Subjects:	Anti-Bacterial Agents - pharmacology Antibiotics beta-Lactamases - genetics Drug resistance Drug Resistance, Multiple, Bacterial - genetics Enzymes Epidemiology Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Genes Gram-negative bacteria Gram-Negative Bacteria - drug effects Gram-Negative Bacteria - genetics Humans Nicaragua Public health Nicaragua
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Antimicrobial resistance (AMR) is a global public health crisis. Much of the burden of AMR in resource-limited settings remains unknown. This pilot study characterized clinical isolates of multidrug-resistant Gram-negative rods (MDR-GNRs) from Nicaragua. New Delhi metallo-β-lactamase (NDM) carbapenemase genes were detected in 60% of isolates. had the highest rates of NDM detection, with 92% (50/54 isolates) positive by polymerase chain reaction (PCR). Pulsed-field gel electrophoresis (PFGE) analysis revealed patterns of clustering among isolates by two factors: plasmid profiles and year of culture. These findings of very high rates of NDM-carbapenemase genes in MDR-GNRs from hospitals throughout Nicaragua are alarming. Further research is needed to determine clinical and epidemiologic factors associated with multidrug-resistant isolates and to guide interventions to limit further spread.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Authors’ addresses: Alexandra W. Dretler, Michelle N. Hargita, Jesse J. Waggoner, and Sarah W. Satola, Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, E-mails: adretle@emory.edu, mhargit@emory.edu, jesse.waggoner@emoryhealthcare.org, and ssatola@emory.edu. Julissa Avila and Lissette Sandoval Lira, Departamento de Bacteriología, Centro Nacional de Diagnóstico y Referencia, Ministerio de Salud, Managua, Nicaragua, E-mails: avilajulissa@hotmail.com and caroli295@yahoo.es. Marcia García Rener, Sustainable Sciences Institute, Managua, Nicaragua, E-mail: maryrener@gmail.com. Raquel Burger-Calderon, Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, CA, E-mail: rqburger@gmail.com. Alexander M. Page, Foundation for Atlanta Veterans Education and Research, Veterans Affairs Medical Center, Atlanta, GA, E-mail: ampage@emory.edu.
ISSN:	0002-9637 1476-1645
DOI:	10.4269/ajtmh.19-0561